VCAM-1/CD106 Antibody (HAE-2Z) [Unconjugated]

809-VR) was serially diluted and captured by Mouse Anti-Human VCAM‑1/CD106 Monoclonal Antibody (Catalog # MAB809) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human VCAM‑1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF809) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" > 809-VR) was serially diluted and captured by Mouse Anti-Human VCAM‑1/CD106 Monoclonal Antibody (Catalog # MAB809) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human VCAM‑1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF809) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" title="Recombinant Human VCAM‑1/CD106 (Catalog # 809-VR) was serially diluted and captured by Mouse Anti-Human VCAM‑1/CD106 Monoclonal Antibody (Catalog # MAB809) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human VCAM‑1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF809) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" />

Recombinant Human VCAM‑1/CD106 (Catalog # <a class=

• Reactivity: Hu
• Applications: ELISA(Cap)
• Clone: HAE-2Z
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

VCAM-1/CD106 Antibody (HAE-2Z) [Unconjugated] Summary

• Immunogen: Human VCAM-1
• Specificity: Detects recombinant human VCAM-1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: VCAM1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Detection: Human VCAM-1/CD106 Biotinylated Antibody (Catalog number BAF809)
  Standard: Recombinant Human VCAM-1/CD106 (Catalog number 809-VR)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VCAM-1/CD106 Antibody (HAE-2Z) [Unconjugated]

• CD106 antigen
• CD106
• DKFZp779G2333
• INCAM-100
• L1CAM
• MGC99561
• vascular cell adhesion molecule 1
• vascular cell adhesion protein 1
• V-CAM 1
• VCAM1
• VCAM-1

Background

Vascular cell adhesion molecule (VCAM-1) is expressed on the surface of activated endothelial cells and macrophages. It binds to leukocyte integrins VLA-4 and  alpha 4 beta 7 to promote leukocyte adhesion at sites of inflamed vasculature.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VCAM-1/CD106 Antibody (MAB809)(8)

Publications using MAB809

• Morten Hjuler Nielsen, Rikke Bæk, Malene Moller Jorgensen, Maiken Mellergaard, Aase Handberg Increased extracellular vesicles (EVs) related to T cell-mediated inflammation and vascular function in familial hypercholesterolemia Atherosclerosis Plus 9/1/2023 [PMID: 37637934]
• Prenylated Polyphenols from Clusiaceae and Calophyllaceae with Immunomodulatory Activity on Endothelial Cells PLoS ONE, 2016-12-01;11(12):e0167361. 2016-12-01 [PMID: 27907087] (ELISA Development, Human)
• Baek R, Sondergaard E, Varming K, Jorgensen M The impact of various preanalytical treatments on the phenotype of small extracellular vesicles in blood analyzed by protein microarray. J Immunol Methods, 2016-08-24;438(0):11-20. 2016-08-24 [PMID: 27568281] (Flow Cytometry, Human)
• Jorgensen MM, Baek R, Varming K. Potentials and capabilities of the Extracellular Vesicle (EV) Array. J Extracell Vesicles 1/1/2015 [PMID: 25862471]
• Stephanie Traub, Alexandra Nikonova, Alan Carruthers, Rebecca Dunmore, Katherine A. Vousden, Leila Gogsadze et al. An Anti-Human ICAM-1 Antibody Inhibits Rhinovirus-Induced Exacerbations of Lung Inflammation PLoS Pathogens 8/1/2013 [PMID: 23935498]
• Stephanie Traub, Alexandra Nikonova, Alan Carruthers, Rebecca Dunmore, Katherine A. Vousden, Leila Gogsadze et al. An Anti-Human ICAM-1 Antibody Inhibits Rhinovirus-Induced Exacerbations of Lung Inflammation PLoS Pathogens 2013-08-01 [PMID: 23935498] (ELISA Detection, N/A)
• Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis. J. Immunol. Methods, 2012-02-17;378(1):72-80. 2012-02-17 [PMID: 22366959] (ELISA Development, Human)
• Otterdal K, Smith C, Oie E, Pedersen TM, Yndestad A, Stang E, Endresen K, Solum NO, Aukrust P, Damas JK Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes. Blood, 2006-08-01;108(3):928-35. 2006-08-01 [PMID: 16861346] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: VCAM1