VCAM-1/CD106 Antibody (BBIG-V3 (IE10)) [Fluorescein]

Anti-human VCAM-1/CD106 staining of HUT-78 cells using either isotype control (Catalog # IC003F; open histogram) or with anti-VCAM 1-Fluorescein (Catalog # BBA22; filled histogram).

• Reactivity: Hu
• Applications: Flow
• Clone: BBIG-V3 (IE10)
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Fluorescein
• Concentration: LYOPH

VCAM-1/CD106 Antibody (BBIG-V3 (IE10)) [Fluorescein] Summary

• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: VCAM1

Applications/Dilutions

• Dilutions:
  • Flow Cytometry

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide
• Concentration: LYOPH

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VCAM-1/CD106 Antibody (BBIG-V3 (IE10)) [Fluorescein]

• CD106 antigen
• CD106
• DKFZp779G2333
• INCAM-100
• L1CAM
• MGC99561
• vascular cell adhesion molecule 1
• vascular cell adhesion protein 1
• V-CAM 1
• VCAM1
• VCAM-1

Background

Human Vascular Cell Adhesion Molecule-1 (VCAM-1/CD106) is a 100 - 110 kDa, 715 amino acid, type I transmembrane glycoprotein (1 - 3). A number of variants of human VCAM-1 occur as a result of alternate gene splicing (1). Moreover, a soluble form of VCAM-1 has been identified in culture supernatants (4), blood (5 - 7) and cerebrospinal fluid (7, 8). Various proteases, including MMPs, neutrophil elastase, and cathepsin B have been implicated in the shedding of transmembrane VCAM-1 (9, 10). VCAM-1 is expressed constitutively on non-vascular cells including: dendritic cells in lymphoid tissues and skin (11), macrophages (11), fibroblasts (12), melanoma cells (11), smooth muscle cells (13), adult satellite muscle cells (14), bone marrow stromal cells (15), chondrocytes (16), mesothelium (17), renal tubular epithelium and mesangium (18), embryonic myoblasts and myotubules (14), neurons (19) and choroid plexus epithelium (20). VCAM-1 expression on vascular endothelium can be induced by a number of inflammatory stimuli (11).

Functionally, VCAM-1 mediates cell adhesion and signal transduction by binding to its ligands/counter-receptors. Ligands for VCAM-1 are the integrins alpha ₄₁ (CD49d/CD29 or VLA4) and alpha ₄₇ (11, 21 - 24), alpha _D₂ (25, 26), and alpha ₉₁ (27). These VCAM-1 ligands are expressed on a variety of lymphoid cells. Accordingly, VCAM-1/VCAM-1 ligand interactions are key events in the rate and timing of leukocyte extravasation (11). Similarly, VCAM-1 mediates the adhesion of melanoma cells to endothelial cells and may play a role in metastasis (11). VCAM-1 expression on bone marrow stromal cells regulates T and B cell development as well as hematopoietic progenitor cell homing and trafficking (28 - 30). Other proposed roles for VCAM-1 include the regulation of osteoclastogenesis via a cell-to-cell contact mechanism (15), and the induction of sickle cell adherence to vascular endothelial cells during hypoxemia (31). Soluble VCAM-1 has been shown to mediate angiogenesis and is chemotactic for T lymphocytes and monocytes (11, 32).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VCAM-1/CD106 Antibody (BBA22)(7)

Publications using BBA22

• SH Chan, CH Hung, JY Shih, PM Chu, YH Cheng, HC Lin, KL Tsai SIRT1 inhibition causes oxidative stress and inflammation in patients with coronary artery disease Redox Biol, 2017-06-02;13(0):301-309. 2017-06-02 [PMID: 28601780] (Flow Cytometry, Human)
• Wang YI, Bettaieb A, Sun C et al. Triglyceride-rich lipoprotein modulates endothelial vascular cell adhesion molecule (VCAM)-1 expression via differential regulation of endoplasmic reticulum stress. PLoS One. [PMID: 24205197]
• Brady K, Dickinson S, Guillot P, Polak J, Blom A, Kafienah W, Hollander A Human fetal and adult bone marrow-derived mesenchymal stem cells use different signaling pathways for the initiation of chondrogenesis. Stem Cells Dev, 2013-12-04;23(5):541-54. 2013-12-04 [PMID: 24172175] (Flow Cytometry, Human)
• Ying I. Wang, John Schulze, Nadine Raymond, Tyler Tomita, Kayan Tam, Scott I. Simon et al. Endothelial inflammation correlates with subject triglycerides and waist size after a high-fat meal American Journal of Physiology-Heart and Circulatory Physiology 2011-03-01 [PMID: 21169396]
• Huang J, Lin Z, Luo M, Lu C, Kim MH, Yu B, Gu J Sinomenine suppresses TNF-alpha-induced VCAM-1 expression in human umbilical vein endothelial cells. J Ethnopharmacol, 2007-08-11;114(2):180-5. 2007-08-11 [PMID: 17869461] (Flow Cytometry, Human)
• Lucerna M, Zernecke A, de Nooijer R, de Jager SC, Bot I, van der Lans C, Kholova I, Liehn EA, van Berkel TJ, Yla-Herttuala S, Weber C, Biessen EA Vascular endothelial growth factor-A induces plaque expansion in ApoE knock-out mice by promoting de novo leukocyte recruitment. Blood, 2006-09-21;109(1):122-9. 2006-09-21 [PMID: 16990600] (ICC, Human)
• Gutierrez G, Mendoza C, Zapata E, Montiel A, Reyes E, Montano LF, Lopez-Marure R Dehydroepiandrosterone inhibits the TNF-alpha-induced inflammatory response in human umbilical vein endothelial cells. Atherosclerosis, 2006-03-30;190(1):90-9. 2006-03-30 [PMID: 16574124] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: VCAM1