VCAM-1/CD106 Antibody (BBIG-V1) [Unconjugated]

VCAM-1/CD106 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Human VCAM-1/CD106 Monoclonal Antibody (Catalog # BBA5) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.This antibody is not suitable for labeling formalin-fixed, paraffin-embedded tissue.

VCAM‑1/CD106 was detected in immersion fixed HUVEC cells treated with TNF alpha (positive) and untreated HUVEC cells (negative) using Mouse Anti-Human VCAM‑1/CD106 Monoclonal Antibody (Catalog # BBA5) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

CD151 mediates lymphocyte binding to human liver tissue and colocalizes with VCAM-1 in primary human liver endothelium. A: representative low- and high-power images of static Stamper–Woodruff adhesion assays of cell tracker green-labeled peripheral blood lymphocytes binding to human liver tissue demonstrating adhesion to the sinusoidal regions within the liver parenchyma; cell nuclei were stained with DAPI. Dashed lines outline a fibrous septum, and arrows highlight lymphocytes within the parenchyma. B: sections were treated with anti-CD151 antibody or appropriate class-matched control antibody, and the results of 4 independent experiments are shown as the number of adherent cells per low-power image. C: dual-color immunofluorescent staining of TNF-alpha -treated HSECs with CD151 (green), VCAM-1 (red), and DAPI (nucleus, blue). Insets: background fluorescence for each class-matched control antibody. Bar = 200 μm (A), 50 μm (B), 10 μm (C). *P < 0.05. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28473332), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, IP, B/N, ICC/IF
• Clone: BBIG-V1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

VCAM-1/CD106 Antibody (BBIG-V1) [Unconjugated] Summary

• Immunogen: Activated HUVEC human umbilical vein endothelial cells
• Specificity: This antibody stains COS cells transfected with human VCAM-1. It does not stain COS cells transfected with E-Selectin, ICAM-1, or PECAM‑1.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: VCAM1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Adhesion Blockade
  • Immunocytochemistry 8-25 ug/mL
  • Immunoprecipitation 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VCAM-1/CD106 Antibody (BBIG-V1) [Unconjugated]

• CD106 antigen
• CD106
• DKFZp779G2333
• INCAM-100
• L1CAM
• MGC99561
• vascular cell adhesion molecule 1
• vascular cell adhesion protein 1
• V-CAM 1
• VCAM1
• VCAM-1

Background

Vascular cell adhesion molecule 1 (VCAM-1) is expressed on the surface of activated endothelial cells and macrophages. It binds to leukocyte integrins VLA-4 and alpha 4 beta 7 to promote leukocyte adhesion at sites of inflamed vasculature.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VCAM-1/CD106 Antibody (BBA5)(49)

Publications using BBA5

• Liu, Y;Ye, SY;He, S;Chi, DM;Wang, XZ;Wen, YF;Ma, D;Nie, RC;Xiang, P;Zhou, Y;Ruan, ZH;Peng, RJ;Luo, CL;Wei, PP;Lin, GW;Zheng, J;Cui, Q;Cai, MY;Yun, JP;Dong, J;Mai, HQ;Xia, X;Bei, JX; Single-cell and spatial transcriptome analyses reveal tertiary lymphoid structures linked to tumour progression and immunotherapy response in nasopharyngeal carcinoma Nature communications 2024-09-04 [PMID: 39231979] (Neutralization, Human)
• Suehiro, JI;Kimura, T;Fukutomi, T;Naito, H;Kanki, Y;Wada, Y;Kubota, Y;Takakura, N;Sakurai, H; Endothelial specific LAT1 ablation normalizes tumor vasculature JCI insight 2024-08-20 [PMID: 39163136] (Western Blot, Human)
• Park, CS;Yoshihara, H;Gao, Q;Qu, C;Iacobucci, I;Ghate, PS;Connelly, JP;Pruett-Miller, SM;Wagner, B;Robinson, CG;Mishra, A;Peng, J;Yang, L;Rankovic, Z;Finkelstein, D;Luger, S;Litzow, M;Paietta, EM;Hebbar, N;Velasquez, MP;Mullighan, CG; Stromal-induced epithelial-mesenchymal transition induces targetable drug resistance in acute lymphoblastic leukemia Cell reports 7/14/2023 [PMID: 37453060]
• Orianne Constant, Ghizlane Maarifi, Jonathan Barthelemy, Marie-France Martin, Bachirou Tinto, Giovanni Savini et al. Differential effects of Usutu and West Nile viruses on neuroinflammation, immune cell recruitment and blood–brain barrier integrity Emerging Microbes & Infections 12/31/2023 [PMID: 36495563]
• Orianne Constant, Ghizlane Maarifi, Jonathan Barthelemy, Marie-France Martin, Bachirou Tinto, Giovanni Savini et al. Differential effects of Usutu and West Nile viruses on neuroinflammation, immune cell recruitment and blood–brain barrier integrity Emerging Microbes & Infections 2023-12-31 [PMID: 36495563] (Flow Cytometry, Insect - Aedes albopictus (Asian tiger mosquito))
• Michael R. Blatchley, Franklyn Hall, Dimitris Ntekoumes, Hyunwoo Cho, Vidur Kailash, Rafael Vazquez‐Duhalt et al. Discretizing Three‐Dimensional Oxygen Gradients to Modulate and Investigate Cellular Processes Advanced Science 7/1/2021 [PMID: 34151527]
• Masahiro Takeuchi, Paula T Vidigal, Mateus T Guerra, Melanie A Hundt, Marie E Robert, Maria Olave-Martinez et al. Neutrophils interact with cholangiocytes to cause cholestatic changes in alcoholic hepatitis Gut 11/19/2020 [PMID: 33214166]
• M Clé, C Desmetz, J Barthelemy, MF Martin, O Constant, G Maarifi, V Foulongne, K Bolloré, Y Glasson, F De Bock, M Blaquiere, L Dehouck, N Pirot, E Tuaillon, S Nisole, F Najioullah, P Van de Per, A Cabié, N Marchi, F Gosselet, Y Simonin, S Salinas Zika Virus Infection Promotes Local Inflammation, Cell Adhesion Molecule Upregulation, and Leukocyte Recruitment at the Blood-Brain Barrier MBio, 2020-08-04;11(4):. 2020-08-04 [PMID: 32753493] (Neutralization, Human)
• Nora M. Schacher, Dorette Raaz‑Schrauder, Francesca Pasutto, Florian M. Stumpfe, Miyuki Tauchi, Barbara Dietel et al. Impact of single nucleotide polymorphisms in the VEGFR2 gene on endothelial cell activation under non‑uniform shear stress International Journal of Molecular Medicine 8/5/2019 [PMID: 31432097]
• Nora M. Schacher, Dorette Raaz‑Schrauder, Francesca Pasutto, Florian M. Stumpfe, Miyuki Tauchi, Barbara Dietel et al. Impact of single nucleotide polymorphisms in the VEGFR2 gene on endothelial cell activation under non‑uniform shear stress International Journal of Molecular Medicine 2019-08-05 [PMID: 31432097] (Immunocytochemistry, Human)
• Yousef H, Czupalla CJ, Lee D et al. Aged blood impairs hippocampal neural precursor activity and activates microglia via brain endothelial cell VCAM1. Nat Med. 2019 May 13 [PMID: 31086348] (ICC/IF, IP, Mouse)
• Yousef H, Czupalla CJ, Lee D et al. Aged blood impairs hippocampal neural precursor activity and activates microglia via brain endothelial cell VCAM1. Nat Med 2019 May 13 [PMID: 31086348] (ICC/IF, IP, Mouse)
• Gareth W. Fearnley, Izma Abdul-Zani, Antony M. Latham, Monica C. Hollstein, John E. Ladbury, Stephen B. Wheatcroft et al. Tpl2 is required for VEGF-A-stimulated signal transduction and endothelial cell function Biology Open 5/17/2019 [PMID: 31072823]
• SM Koura, M Salama, M El-Hussiny, MEA Khalil, A Lotfy, SA Hassan, SA Gad Elhak, MA Sobh Fluoxetine induces direct inhibitory effects on mesenchymal stem cell?derived osteoprogenitor cells independent of serotonin concentration Mol Med Rep, 2019-02-01;0(0):. 2019-02-01 [PMID: 30720108] (Flow Cytometry, Rat)
• A Li, JH Cho, B Reid, CC Tseng, L He, P Tan, CY Yeh, P Wu, Y Li, RB Widelitz, Y Zhou, M Zhao, RH Chow, CM Chuong Calcium oscillations coordinate feather mesenchymal cell movement by SHH dependent modulation of gap junction networks Nat Commun, 2018-12-18;9(1):5377. 2018-12-18 [PMID: 30560870]
• NF Luz, R Khouri, J Van Weyenb, DL Zanette, PP Fiuza, A Noronha, A Barral, VS Boaventura, DB Prates, FK Chan, BB Andrade, VM Borges Leishmania braziliensis Subverts Necroptosis by Modulating RIPK3 Expression Front Microbiol, 2018-09-28;9(0):2283. 2018-09-28 [PMID: 30323793]
• J Meng, C Zhou, B Hu, M Luo, Y Yang, Y Wang, W Wang, G Jiang, J Hong, S Li, H Wu, S Yan, W Yan Stevioside Prevents Wear Particle-Induced Osteolysis by Inhibiting Osteoclastogenesis and Inflammatory Response via the Suppression of TAK1 Activation Front Pharmacol, 2018-09-26;9(0):1053. 2018-09-26 [PMID: 30319406]
• LE Byrnes, DM Wong, M Subramania, NP Meyer, CL Gilchrist, SM Knox, AD Tward, CJ Ye, JB Sneddon Lineage dynamics of murine pancreatic development at single-cell resolution Nat Commun, 2018-09-25;9(1):3922. 2018-09-25 [PMID: 30254276]
• AM Lager, OG Corradin, JM Cregg, MS Elitt, HE Shick, BLL Clayton, KC Allan, HE Olsen, M Madhavan, PJ Tesar Rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells Nat Commun, 2018-09-13;9(1):3708. 2018-09-13 [PMID: 30213958]
• Patrick Hofmann, Janina Sommer, Kosta Theodorou, Luisa Kirchhof, Ariane Fischer, Yuhuang Li et al. Long non-coding RNA H19 regulates endothelial cell aging via inhibition of STAT3 signalling Cardiovascular Research 1/1/2019 [PMID: 30107531]
• SS Kotha, BJ Hayes, KT Phong, MA Redd, K Bomsztyk, A Ramakrishn, B Torok-Stor, Y Zheng Engineering a multicellular vascular niche to model hematopoietic cell trafficking Stem Cell Res Ther, 2018-03-23;9(1):77. 2018-03-23 [PMID: 29566751] (Neutralization)
• FW Lam, Q Da, B Guillory, MA Cruz Recombinant Human Vimentin Binds to P-Selectin and Blocks Neutrophil Capture and Rolling on Platelets and Endothelium J. Immunol., 2018-01-15;0(0):. 2018-01-15 [PMID: 29335256] (Blocking, Human)
• PJ Stewart-Hu, TP Szasz, ER Jaeger, MD Onken, JA Cooper, SC Morley Technical Advance: New in vitro method for assaying the migration of primary B cells using an endothelial monolayer as substrate J. Leukoc. Biol., 2017-06-21;0(0):. 2017-06-21 [PMID: 28637896] (Flow Cytometry, Human)
• MJ Aldape, A Tao, DD Heeney, ER McIndoo, JM French, D Xu Experimental identification and computational characterization of a novel extracellular metalloproteinase produced by Clostridium sordellii RSC Adv, 2017-03-02;7(23):13928-13938. 2017-03-02 [PMID: 28515901] (Western Blot)
• James C. R. Wadkin, Daniel A. Patten, Sivesh K. Kamarajah, Emma L. Shepherd, Vera Novitskaya, Fedor Berditchevski et al. CD151 supports VCAM-1-mediated lymphocyte adhesion to liver endothelium and is upregulated in chronic liver disease and hepatocellular carcinoma American Journal of Physiology-Gastrointestinal and Liver Physiology 8/1/2017 [PMID: 28473332]
• Hegde M, Mukherjee M, Grada Z, Pignata A, Landi D, Navai S, Wakefield A, Fousek K, Bielamowicz K, Chow K, Brawley V, Byrd T, Krebs S, Gottschalk S, Wels W, Baker M, Dotti G, Mamonkin M, Brenner M, Orange J, Ahmed N Tandem CAR T cells targeting HER2 and IL13Ralpha2 mitigate tumor antigen escape. J Clin Invest, 2016-07-18;126(8):3036-52. 2016-07-18 [PMID: 27427982]
• Inoue-Mochita M, Inoue T, Fujimoto T, Kameda T, Awai-Kasaoka N, Ohtsu N, Kimoto K, Tanihara H p38 MAP kinase inhibitor suppresses transforming growth factor-beta2-induced type 1 collagen production in trabecular meshwork cells. PLoS ONE, 2015-03-23;10(3):e0120774. 2015-03-23 [PMID: 25799097] (ICC, Human)
• Onken , Michael, Li , Jinmei, Cooper , John A Uveal melanoma cells utilize a novel route for transendothelial migration. PLoS ONE, 2014-12-15;9(12):e115472. 2014-12-15 [PMID: 25506912] (ICC, Neutralization, Human)
• Roe , Kelsey, Orillo , Beverly, Verma , Saguna West Nile virus-induced cell adhesion molecules on human brain microvascular endothelial cells regulate leukocyte adhesion and modulate permeability of the in vitro blood-brain barrier model. PLoS ONE, 2014-07-18;9(7):e102598. 2014-07-18 [PMID: 25036379] (Neutralization, Human)
• Spilioti E, Jaakkola M, Tolonen T, Lipponen M, Virtanen V, Chinou I, Kassi E, Karabournioti S, Moutsatsou P Phenolic acid composition, antiatherogenic and anticancer potential of honeys derived from various regions in Greece. PLoS ONE, 2014-04-21;9(4):e94860. 2014-04-21 [PMID: 24752205] (ELISA Development (Capture), Human)
• Park , Su-Jung, Kim , Ki-Jo, Kim , Wan-Uk, Cho , Chul-Soo Interaction of mesenchymal stem cells with fibroblast-like synoviocytes via cadherin-11 promotes angiogenesis by enhanced secretion of placental growth factor. J Immunol, 2014-02-26;192(7):3003-10. 2014-02-26 [PMID: 24574497] (Neutralization, Human)
• Lin , Yi-Wen, Huang , Chun-Yao, Chen , Yung-Hsi, Shih , Chun-Min, Tsao , Nai-Wen, Lin , Cheng-Ye, Chang , Nen-Chun, Tsai , Chien-Su, Tsai , Hsiao-Ya, Tsai , Jui-Chi, Huang , Po-Hsun, Li , Chi-Yuan, Lin , Feng-Yen GroEL1, a heat shock protein 60 of Chlamydia pneumoniae, impairs neovascularization by decreasing endothelial progenitor cell function. PLoS ONE, 2013-12-23;8(12):e84731. 2013-12-23 [PMID: 24376840] (Western Blot, Human)
• Lauridsen H, Pober J, Gonzalez A A composite model of the human postcapillary venule for investigation of microvascular leukocyte recruitment. FASEB J, 2013-12-02;28(3):1166-80. 2013-12-02 [PMID: 24297702] (ICC, Human)
• Macrophages induce differentiation of plasma cells through CXCL10/IP-10. J. Exp. Med., 2012-09-17;209(10):1813-23. 2012-09-17 [PMID: 22987802] (Neutralization, Human)
• Benito-Miguel M, Garcia-Carmona Y, Balsa A, Bautista-Caro MB, Arroyo-Villa I, Cobo-Ibanez T, Bonilla-Hernan MG, De Ayala CP, Sanchez-Mateos P, Martin-Mola E, Miranda-Carus ME IL-15 Expression on RA Synovial Fibroblasts Promotes B Cell Survival. PLoS ONE, 2012-07-09;7(7):e40620. 2012-07-09 [PMID: 22792388] (Neutralization, Human)
• Lon D. Ridgway, Michael D. Wetzel, Jason A. Ngo, Anat Erdreich-Epstein, Dario Marchetti Heparanase – induced GEF-H1 signaling regulates the cytoskeletal dynamics of brain metastatic breast cancer cells Molecular Cancer Research 6/1/2012 [PMID: 22513363]
• Yue Zhao, Deborah A. Howatt, Florence Gizard, Takashi Nomiyama, Hannes M. Findeisen, Elizabeth B. Heywood et al. Deficiency of the NR4A Orphan Nuclear Receptor NOR1 Decreases Monocyte Adhesion and Atherosclerosis Circulation Research 8/20/2010 [PMID: 20558821]
• Crosby HA, Lalor PF, Ross E, Newsome PN, Adams DH Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4. J. Hepatol., 2009-07-30;51(4):734-49. 2009-07-30 [PMID: 19703720] (IHC, Neutralization, Human)
• Smedlund K, Vazquez G Involvement of native TRPC3 proteins in ATP-dependent expression of VCAM-1 and monocyte adherence in coronary artery endothelial cells. Arterioscler. Thromb. Vasc. Biol., 2008-09-11;28(11):2049-55. 2008-09-11 [PMID: 18787184] (ELISA Development, Human)
• Ylostalo J, Bazhanov N, Prockop DJ Reversible commitment to differentiation by human multipotent stromal cells in single-cell-derived colonies. Exp. Hematol., 2008-07-10;36(10):1390-402. 2008-07-10 [PMID: 18619725] (ICC, Human)
• Kang YM, Kim SY, Kang JH LIGHT up-regulated on B lymphocytes and monocytes in rheumatoid arthritis mediates cellular adhesion and metalloproteinase production by synoviocytes. Arthritis Rheum., 2007-04-01;56(4):1106-17. 2007-04-01 [PMID: 17393389] (Western Blot, Human)
• Rajesh M, Mukhopadhyay P, Batkai S, Hasko G, Liaudet L, Drel VR, Obrosova IG, Pacher P Cannabidiol attenuates high glucose-induced endothelial cell inflammatory response and barrier disruption. Am. J. Physiol. Heart Circ. Physiol., 2007-03-23;293(1):H610-9. 2007-03-23 [PMID: 17384130] (Western Blot, Human)
• Coupel S, Moreau A, Hamidou M, Horejsi V, Soulillou JP, Charreau B Expression and release of soluble HLA-E is an immunoregulatory feature of endothelial cell activation. Blood, 2007-04-01;109(7):2806-14. 2007-04-01 [PMID: 17179229] (Western Blot, Human)
• Kelly KA, Allport JR, Yu AM, Sinh S, Sage EH, Gerszten RE, Weissleder R SPARC is a VCAM-1 counter-ligand that mediates leukocyte transmigration. J. Leukoc. Biol., 2006-12-18;81(3):748-56. 2006-12-18 [PMID: 17178915] (Functional Assay, Human)
• Fitau J, Boulday G, Coulon F, Quillard T, Charreau B The adaptor molecule Lnk negatively regulates tumor necrosis factor-alpha-dependent VCAM-1 expression in endothelial cells through inhibition of the ERK1 and -2 pathways. J. Biol. Chem., 2006-04-27;281(29):20148-59. 2006-04-27 [PMID: 16644735] (Western Blot, Human)
• Ramachandran R, Morice AH, Compton SJ Proteinase-activated receptor2 agonists upregulate granulocyte colony-stimulating factor, IL-8, and VCAM-1 expression in human bronchial fibroblasts. Am. J. Respir. Cell Mol. Biol., 2006-02-23;35(1):133-41. 2006-02-23 [PMID: 16498082] (Flow Cytometry, Human)
• Yamamoto H, Nagata M, Sakamoto Y CC chemokines and transmigration of eosinophils in the presence of vascular cell adhesion molecule 1. Ann. Allergy Asthma Immunol., 2005-02-01;94(2):292-300. 2005-02-01 [PMID: 15765748] (ELISA Development, ICC, Human)
• Lawson C, Ainsworth M, Yacoub M, Rose M Ligation of ICAM-1 on endothelial cells leads to expression of VCAM-1 via a nuclear factor-kappaB-independent mechanism. J. Immunol., 1999-03-01;162(5):2990-6. 1999-03-01 [PMID: 10072550] (Western Blot, Human)
• Abe Y, Ballantyne CM, Smith CW Functions of domain 1 and 4 of vascular cell adhesion molecule-1 in alpha4 integrin-dependent adhesion under static and flow conditions are differentially regulated. J. Immunol., 1996-12-01;157(11):5061-9. 1996-12-01 [PMID: 8943415] (Immunoprecipitation, Human)

Review for VCAM-1/CD106 Antibody (BBA5) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IF Human 01/20/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID: 22740338
• Species: Human

Bioinformatics

• Gene Symbol: VCAM1