Vav-1 Antibody (591529)

Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and CH-1 mouse B cell lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Vav-1 Monoclonal Antibody (Catalog # MAB3786) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Vav-1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 10.

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 591529
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Vav-1 Antibody (591529) Summary

• Immunogen: E. coli-derived recombinant human Vav‑1
  Trp25-Leu228
  Accession # P15498
• Specificity: Detects human and mouse Vav‑1 in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: VAV1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Vav-1 Antibody (591529)

• oncogene vav
• proto-oncogene vav
• vav 1 guanine nucleotide exchange factor
• vav proto-oncogene
• Vav1
• Vav-1
• vav-T
• VAVvav 1 oncogene

Background

The Vav-1 proto-oncogene is a guanine nucleotide exchange factor (GEF) for the low molecular weight GTPases of the Rho/Rac family. Vav-1 promotes intracellular signaling by its multiple protein binding motifs. It is a cytosolic protein primarily expressed in hematopoietic cells and plays an essential role in the proliferation and activation of T and B cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VAV1
• Uniprot:
  • P15498()