Vav-1 Antibody (591529) [DyLight 488]

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 591529
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 488

Vav-1 Antibody (591529) [DyLight 488] Summary

• Immunogen: E. coli-derived recombinant human Vav‑1
  Trp25-Leu228
  Accession # P15498
• Specificity: Detects human and mouse Vav‑1 in Western blots.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: VAV1
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Vav-1 Antibody (591529) [DyLight 488]

• oncogene vav
• proto-oncogene vav
• vav 1 guanine nucleotide exchange factor
• vav proto-oncogene
• Vav1
• Vav-1
• vav-T
• VAVvav 1 oncogene

Background

The Vav family are Rho/Rac guanosine nucleotide exchange factors (GEFs), consisting of three members in mammalian cells (Vav, Vav2, Vav3) and one in nematodes (CelVav) (1). First discovered based on its transforming properties, Vav is expressed mainly in hematopoietic cells and a few non-hematopoietic tissues, such as the pancreas and tooth enamels (2). As a signaling transducer, Vav is involved in T-cell activated transduction of T-cell antigen receptor (TCR). T-cell stimulated and tyrosine phosphorylated Vav acts as a catalyst in the exchange of guanosine nucleotides on Rac-1, a GTP binding protein (3). Using a mouse model, Vav expression has been determined to play an essential role in the cyctosketetal, proliferative, and apoptotic pathways for developing lymphoid cells and its signal response (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VAV1