Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Ramos human Burkitt's lymphoma cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes. PVDF membrane was probed ...read more
Lck phosphorylated at Y394 was detected in immersion fixed Jurkat human acute T cell leukemia cells treated with (upper panel) or without (lower panel) Pervanadate using Mouse Anti-Human Phospho-Lck (Y394) Monoclonal ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Protein A or G purified from hybridoma culture supernatant
Sterile PBS to a final concentration of 0.5 mg/mL.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Lck [p Tyr394] Antibody (755103)
Leukocyte C-terminal Src kinase
Lymphocyte cell-specific protein-tyrosine kinase
lymphocyte-specific protein tyrosine kinase
p56(LSTRA) protein-tyrosine kinase
proto-oncogene tyrosine-protein kinase LCK
T cell-specific protein-tyrosine kinase
T-lymphocyte specific protein tyrosine kinase p56lck
tyrosine-protein kinase Lck
Lck (p56lck) is a 56 kDa
cytosolic phosphoprotein within the Src family of non-receptor tyrosine
kinases. The 509 amino acid (aa) human Lck contains an SH3 domain (aa 61-121),
an SH2 domain (aa 127-224) and a protein kinase domain (aa 251-259). A short
(363 aa) isoform contains alternate sequence starting at aa 348, while a 539 aa
isoform contains inserted sequence after aa 321. Lck interacts with T cell CD4
and CD8 molecules and plays a pivotal role in regulating T cell activation. It
can be overexpressed in cancers and functions as an oncogene. Y394 is the major
site for autophosphorylation that enhances kinase activity, while Y505 is the
major phosphorylation site for negative regulation.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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