Lck [p Tyr394] Antibody (755103)

Images

 
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Ramos human Burkitt's lymphoma cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes. PVDF membrane was probed ...read more
Lck phosphorylated at Y394 was detected in immersion fixed Jurkat human acute T cell leukemia cells treated with (upper panel) or without (lower panel) Pervanadate using Mouse Anti-Human Phospho-Lck (Y394) Monoclonal ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
755103
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Lck [p Tyr394] Antibody (755103) Summary

Immunogen
Phosphopeptide containing the human Lck Y394 site
Accession # P06239
Modification
p Tyr394
Specificity
Detects human Lck when phosphorylated at Y394. Due to high sequence homology, this antibody may cross-react with phosphorylated members of the Src family.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
LCK
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
MAB7500 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lck [p Tyr394] Antibody (755103)

  • EC 2.7.10
  • EC 2.7.10.2
  • Hck-3
  • Lck
  • Lcktkr
  • Leukocyte C-terminal Src kinase
  • LSK
  • LSTRA
  • Lymphocyte cell-specific protein-tyrosine kinase
  • lymphocyte-specific protein tyrosine kinase
  • p56(LSTRA) protein-tyrosine kinase
  • p56lck
  • p56-LCK
  • pp58lck
  • Protein YT16
  • Proto-oncogene Lck
  • proto-oncogene tyrosine-protein kinase LCK
  • T cell-specific protein-tyrosine kinase
  • T-lymphocyte specific protein tyrosine kinase p56lck
  • tyrosine-protein kinase Lck
  • YT16

Background

Lck (p56lck) is a 56 kDa cytosolic phosphoprotein within the Src family of non-receptor tyrosine kinases. The 509 amino acid (aa) human Lck contains an SH3 domain (aa 61-121), an SH2 domain (aa 127-224) and a protein kinase domain (aa 251-259). A short (363 aa) isoform contains alternate sequence starting at aa 348, while a 539 aa isoform contains inserted sequence after aa 321. Lck interacts with T cell CD4 and CD8 molecules and plays a pivotal role in regulating T cell activation. It can be overexpressed in cancers and functions as an oncogene. Y394 is the major site for autophosphorylation that enhances kinase activity, while Y505 is the major phosphorylation site for negative regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lck Antibody (MAB7500)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC, Western Blot.


Filter By Application
ICC
(1)
Western Blot
(6)
All Applications
Filter By Species
Human
(6)
Mouse
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using MAB7500 Applications Species
W Colon-Mora, A Baer, G Lamture, JT Stapleton, JW Fischer, N Bhattarai A short hepatitis C virus NS5A peptide expression by AAV vector modulates human T cell activation and reduces vector immunogenicity Gene Therapy, 2021;0(0):. 2021 [PMID: 34759330] (Western Blot, Human) Western Blot Human
KK Crean-Tate, C Braley, G Dey, E Esakov, C Saygin, A Trestan, DJ Silver, SM Turaga, EV Connor, R DeBernardo, CM Michener, PG Rose, J Lathia, O Reizes Pretreatment with LCK inhibitors chemosensitizes cisplatin-resistant endometrioid ovarian tumors Journal of ovarian research, 2021;14(1):55. 2021 [PMID: 33888137] (Western Blot, Human) Western Blot Human
JVS Ormonde, Z Li, C Stegen, J Madrenas TAOK3 Regulates Canonical TCR Signaling by Preventing Early SHP-1-Mediated Inactivation of LCK J. Immunol., 2018;0(0):. 2018 [PMID: 30373850] (Western Blot, Human) Western Blot Human
A Baer, W Colon-Mora, N Bhattarai Characterization of the effects of immunomodulatory drug fingolimod (FTY720) on human T cell receptor signaling pathways Sci Rep, 2018;8(1):10910. 2018 [PMID: 30026610] (Western Blot, Human) Western Blot Human
J Borlido, S Sakuma, M Raices, F Carrette, R Tinoco, LM Bradley, MA D'Angelo Nuclear pore complex-mediated modulation of TCR signaling is required for na�ve CD4+ T cell homeostasis Nat. Immunol., 2018;0(0):. 2018 [PMID: 29736031] (Western Blot, Mouse) Western Blot Mouse
N Bhattarai, JH McLinden, J Xiang, MM Mathahs, WN Schmidt, TM Kaufman, JT Stapleton Hepatitis C virus infection inhibits a Src-kinase regulatory phosphatase and reduces T cell activation in vivo PLoS Pathog, 2017;13(2):e1006232. 2017 [PMID: 28235043] (Western Blot, Human) Western Blot Human
Clayton K, Haaland M, Douglas-Vail M, Mujib S, Chew G, Ndhlovu L, Ostrowski M T cell Ig and mucin domain-containing protein 3 is recruited to the immune synapse, disrupts stable synapse formation, and associates with receptor phosphatases. J Immunol, 2014;192(2):782-91. 2014 [PMID: 24337741] (ICC, Human) ICC Human

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Bioinformatics

Gene Symbol LCK
Uniprot