VASA Antibody - BSA Free

Immunohistochemistry-Paraffin: VASA Antibody [NBP3-30963] - Immunohistochemical staining of FFPE human testis using DDX antibody at 5 ug/ml visualized using goat anti-rabbit-HRP secondary and DAB substrate at pH 6.2. Pathologist comment: Very good staining on germ cell testicular @ pH6.2, little background on tissue.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

VASA Antibody - BSA Free Summary

• Immunogen: Synthetic peptide derived from the human VASA protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry : 2-5 ug/ml at pH 6
  • Western Blot : 1-5 ug/ml
• Application Notes: .

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Phosphate buffered saline
• Preservative: 0.08% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for VASA Antibody - BSA Free

• DDX4
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
• DEAD box protein 4
• EC 3.6.1
• EC 3.6.4.13
• MGC111074
• probable ATP-dependent RNA helicase DDX4
• Vasa homolog
• VASA
• VASADEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4

Background

VASA is implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. VASA is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DDX4