SPOP Antibody

Images

 
Western Blot: SPOP Antibody [H00008405-B01P] - SPOP inhibits gastric cancer cell proliferation and migration. The expression of SPOP protein in human GC cell lines - MKN45, MKN28, AGS, SGC7901, and human gastric mucosal ...read more
Immunocytochemistry/ Immunofluorescence: SPOP Antibody [H00008405-B01P] - Analysis of purified antibody to SPOP on HeLa cell. (antibody concentration 10 ug/ml)
Western Blot: SPOP Antibody [H00008405-B01P] - Analysis of SPOP expression in transfected 293T cell line by SPOP polyclonal antibody. Lane 1: SPOP transfected lysate(41.14 KDa). Lane 2: Non-transfected lysate.
Western Blot: SPOP Antibody [H00008405-B01P] - SPOP expression in gastric cancer. Representative immunoblotting of SPOP protein in gastric cancer samples. Image collected and cropped by CiteAb from the following ...read more
Genetic Strategies: Knockdown Validated: SPOP Antibody [H00008405-B01P] - SPOP inhibits Hh/Gli signaling activity. SPOP promotes Gli2 degradation through proteasome pathway. MKN45 cells were treated with vehicle ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

SPOP Antibody Summary

Immunogen
SPOP (NP_001007227.1, 1 a.a. - 374 a.a.) full-length human protein. MSRVPSPPPPAEMSSGPVAESWCYTQIKVVKFSYMWTINNFSFCREEMGEVIKSSTFSSGANDKLKWCLRVNPKGLDEESKDYLSLYLLLVSCPKSEVRAKFKFSILNAKGEETKAMESQRAYRFVQGKDWGFKKFIRRDFLLDEANGLLPDDKLTLFCEVSVVQDSVNISGQNTMNMVKVPECRLADELGGLWENSRFTDCCLCVAGQEFQAHKAILAARSPVFSAMFEHEMEESKKNRVEINDVEPEVFKEMMCFIYTGKAPNLDKMADDLLAAADKYALERLKVMCEDALCSNLSVENAAEILILADLHSADQLKTQAVDFINYHASDVLETSGWKSMVVSHPHLVAEAYRSLASAQCPFLGPPRKRLKQS
Specificity
SPOP - speckle-type POZ protein,
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
SPOP
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Western Blot 1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It has been used for IF. Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 24912477).
Publications
Read Publications using
H00008405-B01P in the following applications:

  • 1 publication
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SPOP Antibody

  • HIB homolog 1
  • Roadkill homolog 1
  • speckle-type POZ protein
  • TEF2

Background

This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SPOP Antibody (H00008405-B01P)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SPOP Antibody (H00008405-B01P) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on SPOP

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Bioinformatics

Gene Symbol SPOP
Entrez
Uniprot