MDMX Antibody

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Western Blot: MDMX Antibody [NB100-556] - Detection of Human HdmX/MDM4 by Western Blot. Samples: Whole cell lysate from Jurkat cells (50, 15, 5 ug) prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit ...read more
Western Blot: MDMX Antibody [NB100-556] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-HdmX/MDM4 antibody NB100-556 ...read more
Western Blot: MDMX Antibody [NB100-556] - Analysis using the Biotin conjugate of NB100-556. Detection of Jurkat (50, 15, and 5 ug). Antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

       

Order Details

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Catalog# & Conjugate Size Price

MDMX Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 125 and 175 of human ortholog of mouse double minute 4 using the numbering given in Swiss-Prot entry O15151 (GeneID 4194).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MDM4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000-1:5000
  • Immunoprecipitation 2 -10 ug/mg lysate
Positive Control
CC2D1A Lysate (NBL1-08754)
Publications
Read Publications using NB100-556.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MDMX Antibody

  • DKFZp781B1423
  • double minute 4 homolog
  • Double minute 4 protein
  • double minute 4, human homolog of; p53-binding protein
  • HDMX
  • Mdm2-like p53-binding protein
  • Mdm4 p53 binding protein homolog (mouse)
  • Mdm4, transformed 3T3 cell double minute 4, p53 binding protein (mouse)
  • Mdm4, transformed 3T3 cell double minute 4, p53 binding protein
  • MDM4-related protein 1
  • MDMXMGC132766
  • mouse double minute 4, human homolog of; p53-binding protein
  • MRP1
  • p53-binding protein Mdm4
  • protein Mdm4
  • Protein Mdmx

Background

HdmX/MDM-4 is the human homolog of the murine double minute 2 (Mdm2) protein. It is a critical negative regulator of the p53 tumor suppressor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-556
Species: Hu
Applications: WB, IP

Publications for MDMX Antibody (NB100-556)(32)


Showing Publications 1 - 10 of 32. Show All 32 Publications.
Publications using NB100-556 Applications Species
McEvoy J, Ulyanov A, Brennan R et al. Analysis of MDM2 and MDM4 single nucleotide polymorphisms, mRNA splicing and protein expression in retinoblastoma. PLoS One 2012 [PMID: 22916154]
Mandke P, Wyatt N, Fraser J et al. MicroRNA-34a modulates MDM4 expression via a target site in the open reading frame. PLoS One 2012 [PMID: 22870278]
Leventaki V, Rodic V, Tripp SR et al. TP53 pathway analysis in paediatric Burkitt lymphoma reveals increased MDM4 expression as the only TP53 pathway abnormality detected in a subset of cases. Br J Haematol 2012 Sep [PMID: 22845047]
Henze J, Muhlenberg T, Simon S et al. p53 modulation as a therapeutic strategy in gastrointestinal stromal tumors. PLoS One 2012 [PMID: 22662219]
Lee JH, Jin Y, He G et al. Hypoxia activates tumor suppressor p53 by inducing ATR-Chk1 kinase cascade-mediated phosphorylation and consequent 14-3-3gammainactivation of MDMX protein. J Biol Chem 2012 Jun [PMID: 22556425]
Biderman L, Poyurovsky MV, Assia Y et al. MdmX is required for p53 interaction with and full induction of the Mdm2 promoter after cellular stress. Mol Cell Biol 2012 Apr [PMID: 22290440]
Wang X, Wang J, Jiang X et al. MdmX protein is essential for Mdm2 protein-mediated p53 polyubiquitination. J Biol Chem 2011 Jul [PMID: 21572037]
Li D, Marchenko ND, Schulz R et al. Functional inactivation of endogenous MDM2 and CHIP by HSP90 causes aberrant stabilization of mutant p53 in human cancer cells. Mol Cancer Res 2011 May [PMID: 21478269]
Anderson VE, Walton MI, Eve PD et al. CCT241533 is a potent and selective inhibitor of CHK2 that potentiates the cytotoxicity of PARP inhibitors. Cancer Res 2011 Jan [PMID: 21239475]
Lee JH, Lu H. 14-3-3Gamma inhibition of MDMX-mediated p21 turnover independent of p53. J Biol Chem 2011 Feb [PMID: 21148311]
Show All 32 Publications.

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol MDM4
Entrez
Uniprot