USP4 Antibody - BSA Free

Western Blot: USP4 Antibody [NB100-2869] - Detection of Human and Mouse USP4 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP4 antibody NB100-2869 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation: USP4 Antibody [NB100-2869] - Detection of human USP4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP4 antibody NB100-2869 used for IP at 6 ug per reaction. USP4 was also immunoprecipitated by rabbit anti-USP4 antibody NB100-2868. For blotting immunoprecipitated USP4, NB100-2869 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

USP4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit USP4 Antibody - BSA Free (NB100-2869) is a polyclonal antibody validated for use in WB and IP. Anti-USP4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 925 and the C-terminus (residue 963) of human Ubiquitin Specific Protease 4 using the numbering given in entry AAB72237.1 (GeneID 7375).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: USP4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Control:

  USP4 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for USP4 Antibody - BSA Free

• Deubiquitinating enzyme 4
• EC 3.1.2.15
• EC 3.4.19.12
• MGC149848
• MGC149849
• ubiquitin carboxyl-terminal esterase 4
• ubiquitin carboxyl-terminal hydrolase 4
• ubiquitin specific peptidase 4 (proto-oncogene)
• ubiquitin specific protease 4 (proto-oncogene)
• ubiquitin thioesterase 4
• Ubiquitin thiolesterase 4
• ubiquitin-specific processing protease 4
• Ubiquitin-specific-processing protease 4
• Ubiquitous nuclear protein homolog
• UNP
• Unph
• UNPUnph
• USP4

Background

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for USP4 Antibody (NB100-2869)(1)

Publication using NB100-2869

• Song EJ, Werner SL, Neubauer J et al. The Prp19 complex and the Usp4Sart3 deubiquitinating enzyme control reversible ubiquitination at the spliceosome. Genes Dev 2010-07-01 [PMID: 20595234]

Bioinformatics

• Gene Symbol: USP4
• Entrez:
  • 7375(Human)
• Uniprot:
  • AAB72237.1(Human)