USP6 Antibody

Immunocytochemistry/Immunofluorescence: USP6 Antibody [NBP2-76350] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Flow Cytometry: USP6 Antibody [NBP2-76350] - Analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunocytochemistry/Immunofluorescence: USP6 Antibody [NBP2-76350] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic/vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

• Reactivity: Hu
• Applications: Flow, ICC/IF, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

USP6 Antibody Summary

• Immunogen: Peptide with sequence C-HHIDLDVRTTLRNH corresponding to internal region (near N-Terminus) according to NP_004496.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: USP6
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/Immunofluorescence 10 ug/ml
  • Peptide ELISA Detection limit 1:4000
• Application Notes: WB: Preliminary experiments in human testis, prostate and placenta lysates gave no specific signal but low background.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Antigen Affinity-purified

Alternate Names for USP6 Antibody

• Deubiquitinating enzyme 6
• EC 3.1.2.15
• EC 3.4.19.12
• HRP1
• hyperpolymorphic gene 1
• Proto-oncogene TRE-2
• TRE17
• tre-2 oncogene
• TRE2
• Tre-2
• TRE2USP6-short
• ubiquitin carboxyl-terminal hydrolase 6
• ubiquitin specific peptidase 6 (Tre-2 oncogene)
• ubiquitin specific peptidase 6-
• ubiquitin specific protease 6 (Tre-2 oncogene)
• ubiquitin thioesterase 6
• Ubiquitin thiolesterase 6
• ubiquitin-specific protease USP6
• Ubiquitin-specific-processing protease 6
• USP6

Background

GenBank Accession Number -P_004496. Gene Ontology terms - lysosomal cysteine-type endopeptidase; nucleic acid binding; deubiquitination; oncogenesis; protein modification.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: USP6