USP14 Antibody (6D6)

Images

 
Western Blot: USP14 Antibody (6D6) [H00009097-M04] - Analysis of USP14 expression in NIH/3T3 (Cat # L018V1).
Immunocytochemistry/ Immunofluorescence: USP14 Antibody (6D6) [H00009097-M04] - Analysis of monoclonal antibody to USP14 on HeLa cell. Antibody concentration 20 ug/ml
Western Blot: USP14 Antibody (6D6) [H00009097-M04] - Analysis of USP14 expression in HeLa (Cat # L013V1).
Western Blot: USP14 Antibody (6D6) [H00009097-M04] - Analysis of USP14 expression in PC-12 (Cat # L012V1).
Western Blot: USP14 Antibody (6D6) [H00009097-M04] - Analysis of USP14 expression in Raw 264.7 (Cat # L024V1).
Western Blot: USP14 Antibody (6D6) [H00009097-M04] - Analysis of USP14 expression in transfected 293T cell line by USP14 monoclonal antibody (M04), clone 6D6. Lane 1: USP14 transfected lysatE (56.1 KDa). Lane 2: ...read more
ELISA: USP14 Antibody (6D6) [H00009097-M04] - Detection limit for recombinant GST tagged USP14 is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
6D6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

USP14 Antibody (6D6) Summary

Immunogen
USP14 (NP_005142, 395 a.a. ~ 495 a.a) partial recombinant protein with GST tag.PQKEVKYEPFSFADDIGSNNCGYYDLQAVLTHQGRSSSSGHYVSWVKRKQDEWIKFDDDKVSIVTPEDILRLSGGGDWHIAYVLLYGPRRVEIMEEESEQ
Specificity
USP14 - ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
USP14
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Immunocytochemistry/Immunofluorescence
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Publications
Read Publications using H00009097-M04.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for USP14 Antibody (6D6)

  • Deubiquitinating enzyme 14
  • EC 3.1.2.15
  • EC 3.4.19.12
  • TGT
  • TGTubiquitin carboxyl-terminal hydrolase 14
  • tRNA-guanine transglycosylase, 60-kD subunit
  • ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
  • ubiquitin specific protease 14 (tRNA-guanine transglycosylase)
  • Ubiquitin Thioesterase 14
  • Ubiquitin thiolesterase 14
  • ubiquitin-specific processing protease 14
  • Ubiquitin-specific-processing protease 14
  • Ubp6
  • USP14

Background

This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for USP14 Antibody (H00009097-M04)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol USP14
Entrez
OMIM
Uniprot