USP4 Antibody

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Immunoprecipitation: USP4 Antibody [NB100-2868] - Detection of Human USP4 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

       

Order Details

USP4 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 225 and 275 of human Ubiquitin Specific Protease 4 using the numbering given in entry AAB72237.1 (GeneID 7375).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
USP4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
Not Recommended for Western Blot. Use rabbit anti-USP4 Antibody NB 100-2868.
Positive Control
USP4 Lysate (NBL1-17663)
Publications
Read Publication using NB100-2868.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for USP4 Antibody

  • Deubiquitinating enzyme 4
  • EC 3.1.2.15
  • EC 3.4.19.12
  • MGC149848
  • MGC149849
  • ubiquitin carboxyl-terminal esterase 4
  • ubiquitin carboxyl-terminal hydrolase 4
  • ubiquitin specific peptidase 4 (proto-oncogene)
  • ubiquitin specific protease 4 (proto-oncogene)
  • ubiquitin thioesterase 4
  • Ubiquitin thiolesterase 4
  • ubiquitin-specific processing protease 4
  • Ubiquitin-specific-processing protease 4
  • Ubiquitous nuclear protein homolog
  • UNP
  • Unph
  • UNPUnph
  • USP4

Background

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for USP4 Antibody (NB100-2868)(1)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol USP4
Entrez
Uniprot