USP33 Antibody - BSA Free

Western Blot: USP33 Antibody [NBP2-20832] - Sample (30 ug of whole cell lysate) A: HepG2 7. 5% SDS PAGE gel, diluted at 1:500.

Immunocytochemistry/Immunofluorescence: USP33 Antibody [NBP2-20832] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

USP33 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit USP33 Antibody - BSA Free (NBP2-20832) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human USP33. The exact sequence is proprietary.
• Predicted Species: Mouse (98%), Rat (98%), Bovine (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: USP33
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 107 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (83%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for USP33 Antibody - BSA Free

• Deubiquitinating enzyme 33
• EC 3.1.2.15
• EC 3.4.19.12
• hVDU1
• KIAA1097
• KIAA1097MGC16868
• pVHL-interacting deubiquitinating enzyme 1
• ubiquitin carboxyl-terminal hydrolase 33
• ubiquitin specific peptidase 33
• ubiquitin thioesterase 33
• Ubiquitin thiolesterase 33
• Ubiquitin-specific-processing protease 33
• USP33
• VDU1
• VDU1ubiquitin specific protease 33
• VHL-interacting deubiquitinating enzyme 1

Background

Deubiquitinating enzyme involved in various processes such as cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in cell migration via its interaction with intracellular domain of ROBO1, leading to regulate the Slit signaling. Plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of ROBO1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (ARRB1 and ARRB2) and beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Mediates deubiquitination of both 'Lys-48'-and 'Lys-63'-linked polyubiquitin chains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: USP33
• Uniprot:
  • Q8TEY7(Human)