USP20 Antibody

Western Blot: USP20 Antibody [NB100-1260] - staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: USP20 Antibody [NB100-1260] - (3ug/ml) IHC analysis of USP20 in paraffin embedded Human Lymph Node using this antibody at 3 ug/ml. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

USP20 Antibody Summary

• Immunogen: Peptide with sequence C-HGEQKIEAETRAV, from the C Terminus of the protein sequence according to NP_006667.3.
• Specificity: Reported variants represent identical protein (NP_006667.3; NP_001103773.2; NP_001008563.2).
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: USP20
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Peptide ELISA detection limit 1:32000
  • Western Blot 1:100-1:2000
• Application Notes: WB: Approx. 100 kDa band observed in human tonsil lysates (calculated MW of 102 kDa band according to NP_006667.3).
  IHC-P: n paraffin embedded Human Lymph Node shows strongest staining in smooth muscle cells surrounding a vessel. Data obtained from a previous batch (different goat).
• Control:

  USP20 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for USP20 Antibody

• Deubiquitinating enzyme 20
• EC 3.4.19.12
• KIAA1003hVDU2
• LSFR3A
• pVHL-interacting deubiquitinating enzyme 2
• ubiquitin carboxyl-terminal hydrolase 20
• ubiquitin specific peptidase 20
• ubiquitin specific protease 20
• ubiquitin thioesterase 20
• Ubiquitin Thiolesterase 20
• Ubiquitin-specific-processing protease 20
• USP20
• VDU2
• VHL-interacting deubiquitinating enzyme 2

Background

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for USP20 Antibody (NB100-1260)(1)

Publication using NB100-1260

• Curcio-Morelli C, Zavacki AM, Christofollete M et al. Deubiquitination of type 2 iodothyronine deiodinase by von Hippel-Lindau protein-interacting deubiquitinating enzymes regulates thyroid hormone activation. J Clin Invest 2003-07-01 [PMID: 12865408]

Bioinformatics

• Gene Symbol: USP20
• Entrez:
  • 10868(Human)
  • 74270(Mouse)
• Uniprot:
  • NP_006667.(Human)