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USP20 Antibody

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Western Blot: USP20 Antibody [NB100-1260] - staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: USP20 Antibody [NB100-1260] - staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: USP20 Antibody [NB100-1260] - (3ug/ml) IHC analysis of USP20 in paraffin embedded Human Lymph Node using this antibody at 3 ug/ml. Microwaved antigen retrieval with Tris/EDTA buffer pH9, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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USP20 Antibody Summary

Immunogen
Peptide with sequence C-HGEQKIEAETRAV, from the C Terminus of the protein sequence according to NP_006667.3.
Specificity
Reported variants represent identical protein (NP_006667.3; NP_001103773.2; NP_001008563.2).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
USP20
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 3 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Peptide ELISA detection limit 1:32000
Application Notes
WB: Approx. 100 kDa band observed in human tonsil lysates (calculated MW of 102 kDa band according to NP_006667.3).
IHC-P: n paraffin embedded Human Lymph Node shows strongest staining in smooth muscle cells surrounding a vessel. Data obtained from a previous batch (different goat).
Positive Control
USP20 Lysate (NBL1-17651)
Publications
Read Publication using NB100-1260.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for USP20 Antibody

  • Deubiquitinating enzyme 20
  • EC 3.4.19.12
  • KIAA1003hVDU2
  • LSFR3A
  • pVHL-interacting deubiquitinating enzyme 2
  • ubiquitin carboxyl-terminal hydrolase 20
  • ubiquitin specific peptidase 20
  • ubiquitin specific protease 20
  • ubiquitin thioesterase 20
  • Ubiquitin Thiolesterase 20
  • Ubiquitin-specific-processing protease 20
  • USP20
  • VDU2
  • VHL-interacting deubiquitinating enzyme 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for USP20 Antibody (NB100-1260)(1)


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Positive Control Lysate(s)

USP20 Overexpression Lysate (Native)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol USP20
Entrez
Uniprot