USP25 Antibody [Alexa Fluor® 750]

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 750

USP25 Antibody [Alexa Fluor® 750] Summary

• Immunogen: USP25 antibody was raised against a 17 amino acid peptide near the center of human USP25. The immunogen is located within amino acids 470 - 520 of USP25. Amino Acid Squence: IPSQTLPSTTEQQGALS
• Specificity: USP25 antibody is human and mouse reactive. Multiple isoforms of USP25 are known to exist.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: USP25
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

0

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Peptide affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for USP25 Antibody [Alexa Fluor® 750]

• Deubiquitinating enzyme 25
• EC 3.1.2.15
• EC 3.4.19.12
• ubiquitin carboxyl-terminal hydrolase 25
• ubiquitin specific peptidase 25
• ubiquitin specific protease USP2510USP21ubiquitin specific protease 25
• ubiquitin thioesterase 25
• Ubiquitin Thiolesterase 25
• ubiquitin-specific processing protease 25
• Ubiquitin-specific-processing protease 25
• USP on chromosome 21
• USP25

Background

USP25 is a ubiquitin-specific protease. Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25 (Valero et al., 1999 [PubMed 10644437]).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: USP25