USP3 Antibody - BSA Free

Immunohistochemistry-Paraffin: USP3 Antibody [NB100-77284] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- USP3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Immunoprecipitation: USP3 Antibody [NB100-77284] - Detection of Human USP3 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-USP3 antibody NB100-77284used for IP at 3 mcg/mg lysate. USP3 was also immunoprecipitated by rabbit anti-USP3 antibody NB100-77283, which recognizes an upstream epitope. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu
• Applications: IHC, IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

USP3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit USP3 Antibody - BSA Free (NB100-77284) is a polyclonal antibody validated for use in IHC and IP. Anti-USP3 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 471 and 521 of human ubiquitin specific protease 3 using the numbering given in entry AAD42992.1 (GeneID 9960).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: USP3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
  • Immunoprecipitation 2-5 ug/mg lysate
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Control:

  USP3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for USP3 Antibody - BSA Free

• Deubiquitinating enzyme 3
• EC 3.1.2.15
• EC 3.4.19.12
• MGC129878
• MGC129879
• SIH003
• ubiquitin carboxyl-terminal hydrolase 3
• ubiquitin specific peptidase 3
• ubiquitin specific protease 3
• ubiquitin thioesterase 3
• Ubiquitin thiolesterase 3
• Ubiquitin-specific-processing protease 3
• UBP
• USP3

Background

Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 3 (USP3) is a member of this family and is involved in the deubiquitination of histones H2A and H2B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for USP3 Antibody (NB100-77284)(2)

Publications using NB100-77284

• Karapurkar JK, Kim MS, Colaco JC et al. CRISPR/Cas9-based genome-wide screening of the deubiquitinase subfamily identifies USP3 as a protein stabilizer of REST blocking neuronal differentiation and promotes neuroblastoma tumorigenesis Journal of experimental & clinical cancer research : CR 2023-05-12 [PMID: 37170124] (Immunohistochemistry-Paraffin, Mouse)
  
  Details:
  IHC-P 1:100
• Das S, Chandrasekaran AP, Suresh B et al. Genome-scale screening of deubiquitinase subfamily identifies USP3 as a stabilizer of Cdc25A regulating cell cycle in cancer Cell Death Differ. 2020-05-15 [PMID: 32415280] (IHC-P, Human)

Bioinformatics

• Gene Symbol: USP3
• Entrez:
  • 9960(Human)
• Uniprot:
  • AAD42992.1(Human)