Recombinant Human/Mouse/Rat NEDD8 Protein, CF

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Recombinant Human/Mouse/Rat NEDD8 Protein, CF Summary

Details of Functionality
Recombinant Human NEDD8 can be conjugated to substrate proteins via the subsequent actions of a NEDD8-activating (E1) enzyme, a NEDD8-conjugating (E2) enzyme, and a NEDD8 ligase (E3). Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human NEDD8 concentration of 10-50 μM.
Source
E. coli-derived NEDD8 protein
Met1 - Gly76
Accession #
Protein/Peptide Type
Recombinant Proteins
Gene
NEDD8
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

Dilutions
  • Enzyme Activity
Theoretical MW
8.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
UL-812 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer
Supplied as a solution in HEPES, NaCl and DTT.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Mouse/Rat NEDD8 Protein, CF

  • FLJ43224
  • MGC104393
  • MGC125896
  • MGC125897
  • NEDD8
  • NEDD-8
  • Neddylin
  • Neural precursor cell expressed developmentally down-regulated protein 8
  • neural precursor cell expressed, developmentally down-regulated 8
  • Rub1
  • Ubiquitin-like protein Nedd8

Background

Neural Precursor Cell Expressed Developmentally Downregulated Gene 8 (NEDD8), also known as Related to Ubiquitin 1 (Rub1), is a 6-8 kDa member of the Ubiquitin family of proteins. Human pro-NEDD8 is 81 amino acids (aa) in length. Pro-NEDD8 contains one Ubiquitin-like domain (aa 1-65) and a 4-5 aa C-terminal propeptide (1,2). Cleavage of the propeptide exposes a C-terminal glycine residue that is used to generate a glycine-lysine intermolecular bond. Mature human NEDD8 is highly conserved and shows 100% aa identity to NEDD8 from several species including mouse, rat, and canine. Human NEDD8 is activated by a distinct NEDD8-activating (E1) enzyme, a heterodimeric complex composed of APPBP1 and UBA3 subunits (3). Activated NEDD8 is subsequently transferred to the UBE2M/Ubc12 or UBE2F NEDD8-conjugating (E2) enzymes (4). Through a process termed neddylation, the ROC1/Rbx1 RING Finger E3 ligase transfers NEDD8 to specific substrates (5). For example, transfer of NEDD8 to the Cullin scaffold activates members of the Cullin-RING Ligase (CRL) family of Ubiquitin ligases (E3) (6-8). CRL-induced ubiquitination is known to regulate proteins that are important for cell cycle progression and cell survival (9,10). Physiologically, NEDD8 plays a critical regulatory role in cell proliferation and dysregulation of the NEDD8 pathway has been associated with several cancer pathologies (11,12).

  1. Kumar, S. et al. (1993) Biochem. Biophys. Res. Commun. 195:393. 
  2. Kamitani, T. et al. (1997) J. Biol. Chem. 272:28557.
  3. Huang, D.T. & B.A. Schulman (2005) Methods Enzymol. 398:9.
  4. Gong, L. & E.T. Yeh (1999) J. Biol. Chem. 274:12036.
  5. Ohki, Y. et al. (2009) Biochem. Biophys. Res. Commun. 381:443.
  6. Petroski, M.D. & R.T. Deshares (2005) Nat. Rev. Mol. Cell Biol. 6:9.
  7. Hjerpe, R. et al. (2012) Biochem. J. 441:927.
  8. Calabrese, M.F. et al. (2011) Nat. Struct. Mol. Biol. 18:947.
  9. Tateishi, K. et al. (2001) J. Cell. Biol. 155:571.
  10. Ohh, M. et al. (2002) EMBO Rep. 3:177.
  11. Luo, Z. et al. (2012) Cancer Res. 72:3360.
  12. Soucy, T.A. et al. (2010) Genes Cancer 1:708.

Publications for NEDD8 (UL-812)(7)

We have publications tested in 2 confirmed species: Human, N/A.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(6)
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Human
(6)
N/A
(1)
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Showing Publications 1 - 7 of 7.
Publications using UL-812 Applications Species
S Banchenko, F Krupp, C Gotthold, J Bürger, A Graziadei, FJ O'Reilly, L Sinn, O Ruda, J Rappsilber, CMT Spahn, T Mielke, IA Taylor, D Schwefel Structural insights into Cullin4-RING ubiquitin ligase remodelling by Vpr from simian immunodeficiency viruses PloS Pathogens, 2021-08-02;17(8):e1009775. 2021-08-02 [PMID: 34339457] (Bioassay, Human) Bioassay Human
AM Cathcart, GH Bird, TE Wales, HD Herce, EP Harvey, ZJ Hauseman, CE Newman, U Adhikary, MS Prew, T Oo, S Lee, JR Engen, LD Walensky Targeting a helix-in-groove interaction between E1 and E2 blocks ubiquitin transfer Nat. Chem. Biol., 2020-08-17;0(0):. 2020-08-17 [PMID: 32807965] (Bioassay, Human, N/A) Bioassay Human, N/A
X Liu, JM Reitsma, JL Mamrosh, Y Zhang, R Straube, RJ Deshaies Cand1-Mediated Adaptive Exchange Mechanism Enables Variation in F-Box Protein Expression Mol. Cell, 2018-03-01;69(5):773-786.e6. 2018-03-01 [PMID: 29499133] (Bioassay, Human) Bioassay Human
H Zhou, J Lu, L Liu, D Bernard, CY Yang, E Fernandez-, K Chinnaswam, S Layton, J Stuckey, Q Yu, W Zhou, Z Pan, Y Sun, S Wang A potent small-molecule inhibitor of the DCN1-UBC12 interaction that selectively blocks cullin 3 neddylation Nat Commun, 2017-10-27;8(1):1150. 2017-10-27 [PMID: 29074978] (Bioassay, Human) Bioassay Human
Huang G, Towe C, Choi L, Yonekawa Y, Bommelje C, Bains S, Rechler W, Hao B, Ramanathan Y, Singh B The ubiquitin-associated (UBA) domain of SCCRO/DCUN1D1 protein serves as a feedback regulator of biochemical and oncogenic activity. J Biol Chem, 2014-11-19;290(1):296-309. 2014-11-19 [PMID: 25411243] (Bioassay, Human) Bioassay Human
Huang G, Stock C, Bommelje C, Weeda V, Shah K, Bains S, Buss E, Shaha M, Rechler W, Ramanathan S, Singh B SCCRO3 (DCUN1D3) antagonizes the neddylation and oncogenic activity of SCCRO (DCUN1D1). 2014-10-27;0(0):. 2014-10-27 [PMID: 25349211] (Human) Human
Stanley D, Bartholomeeusen K, Crosby D, Kim D, Kwon E, Yen L, Cartozo N, Li M, Jager S, Mason-Herr J, Hayashi F, Yokoyama S, Krogan N, Harris R, Peterlin B, Gross J Inhibition of a NEDD8 Cascade Restores Restriction of HIV by APOBEC3G. PLoS Pathog, 2012-12-27;8(12):e1003085. 2012-12-27 [PMID: 23300442] (Bioassay) Bioassay

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Bioinformatics

Gene Symbol NEDD8
Uniprot