TRAF-5 Antibody - BSA Free

Western Blot: TRAF-5 Antibody [NB100-56178] - TRAF5 in normal human tissues using this antibody. TRAF5 is observed at ~64 kDa. Additional bands of lower molecular weight were seen in some cases, and may represent TRAF5 degradation fragments.

Immunohistochemistry-Paraffin: TRAF-5 Antibody [NB100-56178] - Formalin-fixed, paraffin-embedded mouse tissue stained for TRAF5 expression using NB100-56178 at 1:2000. Hematoxylin-eosin counterstain. A, thymus. B, bone marrow.

Immunohistochemistry: TRAF-5 Antibody [NB100-56178] - Formalin-fixed, paraffin-embedded mouse spleen stained for TRAF5 expression using NB100-56178 at 1:2000. Hematoxylin-eosin counterstain. A1 is a higher magnification of A.

• Reactivity: Hu, Mu, Rt, Ma
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TRAF-5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TRAF-5 Antibody - BSA Free (NB100-56178) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-TRAF-5 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide corresponding to amino acids 1-20 ([BLAST]MAHSEEQAAVPCAFIRQNSG[/BLAST]) of mouse TRAF5 was used as immunogen, GenBank no. AAH12702.1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRAF5
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:50 - 1:200
  • Western Blot 1:1000 - 1:2000
• Control:

  TRAF-5 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for TRAF-5 Antibody - BSA Free

• MGC:39780
• RNF84
• RNF84RING finger protein 84
• TNF receptor-associated factor 5
• TRAF5
• TRAF-5

Background

The TRAF (TNF receptor-associated factor) family is a group of adapter proteins (TRAFs 1-6) that link a wide variety of cell surface receptors to diverse signaling cascades leading to the activation of NF-kB and mitogen-activated protein kinases (reviewed in Chung et al, 2002). TRAFs are major signal transducers for both the TNF and IL- 1/TLR receptor superfamilies and collectively play important functions in both adaptive and innate immunity. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. TRAFs interact with a variety of proteins that regulate receptor-induced cell death or survival, and TRAF-mediated signaling can promote cell survival or interfere with death receptor-induced apoptosis. NB100-56178 recognizes TRAF5. Mouse TRAF5 is a 558 amino acid protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRAF-5 Antibody (NB100-56178)(1)

Publication using NB100-56178

• Zapata JM, Krajewska M, Krajewski S et al. TNFR-associated factor family protein expression in normal tissues and lymphoid malignancies. J Immunol. 2000-11-01 [PMID: 11046039] (WB, IHC-P)
  
  Details:
  WB: Figs 1, 2, 4, 5; IHC-paraffin: Fig 3.

Bioinformatics

• Gene Symbol: TRAF5
• Entrez:
  • 7188(Human)
  • 22033(Mouse)