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TRAF-5 Antibody

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Western Blot: TRAF-5 Antibody [NB100-56178] - TRAF5 in normal human tissues using this antibody. TRAF5 is observed at ~64 kDa. Additional bands of lower molecular weight were seen in some cases, and may represent TRAF5 degradation fragments.
Western Blot: TRAF-5 Antibody [NB100-56178] - TRAF5 in normal human tissues using this antibody. TRAF5 is observed at ~64 kDa. Additional bands of lower molecular weight were seen in some cases, and may represent TRAF5 ...read more
Immunohistochemistry-Paraffin: TRAF-5 Antibody [NB100-56178] - Formalin-fixed, paraffin-embedded mouse tissue stained for TRAF5 expression using NB100-56178 at 1:2000. Hematoxylin-eosin counterstain. A, thymus. B, bone ...read more
Immunohistochemistry: TRAF-5 Antibody [NB100-56178] - Formalin-fixed, paraffin-embedded mouse spleen stained for TRAF5 expression using NB100-56178 at 1:2000. Hematoxylin-eosin counterstain. A1 is a higher magnification ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, MaSpecies Glossary
Applications WB, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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TRAF-5 Antibody Summary

Immunogen
A synthetic peptide corresponding to amino acids 1-20 ([BLAST]MAHSEEQAAVPCAFIRQNSG[/BLAST]) of mouse TRAF5 was used as immunogen, GenBank no. AAH12702.1
Specificity
TRAF5
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRAF5
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:50 - 1:200
Control
TRAF-5 Overexpression Lysate
TRAF-5 Knockout HeLa Cell Lysate
Publications
Read Publication using NB100-56178.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for TRAF-5 Antibody

  • MGC:39780
  • RNF84
  • RNF84RING finger protein 84
  • TNF receptor-associated factor 5
  • TRAF5
  • TRAF-5

Background

The TRAF (TNF receptor-associated factor) family is a group of adapter proteins (TRAFs 1-6) that link a wide variety of cell surface receptors to diverse signaling cascades leading to the activation of NF-kB and mitogen-activated protein kinases (reviewed in Chung et al, 2002). TRAFs are major signal transducers for both the TNF and IL- 1/TLR receptor superfamilies and collectively play important functions in both adaptive and innate immunity. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. TRAFs interact with a variety of proteins that regulate receptor-induced cell death or survival, and TRAF-mediated signaling can promote cell survival or interfere with death receptor-induced apoptosis. NB100-56178 recognizes TRAF5. Mouse TRAF5 is a 558 amino acid protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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210-TA
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
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(649 Publications)
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TRAF-2 Antibody
NB100-56173
Western Blot: TRAF-2 Antibody [NB100-56173] - Analysis of TRAF-2 in normal human tissues using this antibody. TRAF-2 is observed at ~60-64 kDa. Additional bands of lower molecular weight were seen in some cases, and may represent TRAF-2 degradation fragments.
Immunocytochemistry/Immunofluorescence: TRAF-2 Antibody [NB100-56173] - Immunostaining of HN30 and MCF-12a cells for TRAF2 3 days post-transfectionMCF-12a NT (DAPI): non-treated MCF-12a DAPI stained, MCF-12a NT (TRAF2-FITC): non-treated MCF-12a FITC stained for TRAF2, MCF-12a pNSP 5a3a (DAPI): MCF-12a transfected with pcDNA 3.0 NSP 5a3a DAPI stained, MCF-12a pNSP 5a3a (TRAF2-FITC): MCF-12a transfected with pcDNA 3.0 NSP 5a3a FITC stained for Traf-2, HN30 NT (DAPI): non-treated HN30 DAPI stained, HN30 NT (TRAF2-FITC): non-treated HN30 FITC stained for TRAF2, HN30 pNSP 5a3a (DAPI): HN30 transfected with pcDNA 3.0 NSP 5a3a DAPI stained, HN30 pNSP 5a3a (TRAF2-FITC): HN30 transfected with pcDNA 3.0 NSP 5a3a FITC stained for TRAF2. White arrows indicate apoptotic bodies. Image collected and cropped by Cite Ab from the following publication (A novel dual signaling axis for NSP 5a3a induced apoptosis in head and neck carcinoma. Oncotarget (2011) licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: TRAF-2 Antibody [NB100-56173] - Formalin-fixed, paraffin-embedded human colon carcinoma tissue sections stained for TRAF2 using this antibody at 1:2000. Hematoxylin-eosin counterstain.
(3 Publications)
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
TRAF-6 Inhibitor
Peptide Set
NBP2-26506
TRAF6 Inhibitor Peptide Set [NBP2-26506] - The TRAF6 inhibitory peptide inhibits osteoclast ifferentiation: RAW264.7 cells were plated on 24-well plates and stimulated with RANKL and M-CSF and incubated in the presence of different concentrations of control peptide (PTD) or the TRAF6. The TRAF6 at the concentration above 30 uM suppressed osteoclast differentiation.
(15 Publications)
Species: Hu, Mu, Rt
TANK Antibody
NB100-56144
Immunohistochemistry: TANK Antibody - Unpurified [NB100-56144] - TANK Antibody [NB100-56144] - Immunohistochemical analysis of TANK/I-TRAF1 expression in formalin-fixed, paraffin-embedded normal human colon using this antibody at 1:2000. A-A3, low and high magnification images. Hematoxylin-eosin counterstain.
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP
TRAF-3 Antibody
NB100-56176
Immunohistochemistry-Paraffin: TRAF-3 Antibody - Unpurified [NB100-56176] - Analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using this at 1:4000 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP
NFkB p105/p50
Antibody (2J10D7)
NB100-56583
Western Blot: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - Analysis of p50 in HeLa lysate in the A) absence and B) presence of immunizing peptide using p50 antibody at 5 ug/ml.
Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - IHC-P of rabbit aorta using NB100-56583. Secondary antibody: anti-mouse histofine ( Nisherei Bioscience Inc. ref: 414131F). Development: DAB (Dako ref: K346811-2) and counterstained with Hematoxylin. Submitted via verified customer review
Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on U937 cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.
(1 Review)
(4 Publications)
Species: Hu, Rt, Rb
Applications: WB, Flow, IHC, IHC-P, KO
TRAF-1 Antibody
NB100-56170
Western Blot: TRAF-1 Antibody [NB100-56170] - Analysis of TRAF-1 in normal human tissues using this antibody. TRAF-1 is observed at 50-50 kDa. Additional bands of lower molecular weight were seen in some cases, and may represent TRAF-1 degradation fragments.
Immunohistochemistry-Paraffin: TRAF-1 Antibody [NB100-56170] - Formalin-fixed, paraffin-embedded human tissue sections stained for TRAF-1 expression using NB100-56170 at 1:2000. Hematoxylin-eosin counterstain. A, A1: Formalin-fixed, paraffin-embedded human lymphoma tissue section stained for TRAF-1 expression using NB100-56170 at 1:2000. Hematoxylin-eosin counterstain. A, Hodgkin'sDisease, the Reed-Sternberg cells are positive for TRAF-1 expression. B, lymph node, dendritic-like cells are positive for TRAF-1 expression. A1 and B1 are higher magnifications from A and B, respectively.
Immunohistochemistry-Paraffin: TRAF-1 Antibody [NB100-56170] - Formalin-fixed, paraffin-embedded human lymphoma tissue section stained for TRAF-1 expression using NB100-56170 at 1:2000. Hematoxylin-eosin counterstain. A1 is a higher magnification of A.
(1 Publication)
Species: Hu, Bv, Ca
Applications: WB, IHC, IHC-P, IP
CD40/TNFRSF5
Antibody (mAb89)
DDX0131P-100
Immunocytochemistry/Immunofluorescence: CD40/TNFRSF5 Antibody (mAb89) [DDX0131P-100] - CD40-transfected L cells stained.
Immunohistochemistry: CD40/TNFRSF5 Antibody (mAb89) [DDX0131P-100] - IHC staining on Bouin paraffinlymph node section. HumanCD40 stained with mAb 89.
(11 Publications)
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Applications: Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P
JNK1 Antibody
(228601) [Unconjugate
MAB17761
Western blot shows lysates of CHP-100 human neuroblastoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for JNK1 was detected at approximately 46 kDa and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Western blot shows recombinant human JNK1, JNK2, and JNK3 (1 ng/lane). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for JNK1 was detected at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
JNK1 was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(2 Reviews)
(3 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC
RANK/TNFRSF11A
Antibody (9A725)
NB100-56508
Western Blot: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Analysis of RANK in transfected 293 cells using RANK antibody at 2 ug/ml.
Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - An intracellular stain was performed on Raw264.7 cells with RANK/TNFRSF11A [9A725] Antibody NB100-56508AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Intracellular flow cytometry analysis of RANK in 10^6 RAW cells using 2 ug of RANK antibody. Shaded histogram represents RAW cells without antibody; green represents isotype control; antibody red represents RANK antibody.
(17 Publications)
Species: Hu, Mu, Rt, Av
Applications: WB, Flow, IHC, IHC-P, CyTOF-ready, Flow-CS
TRAF-4 Antibody
NB100-56177
Immunohistochemistry-Paraffin: TRAF-4 Antibody [NB100-56177] - Formalin-fixed, paraffin embedded, normal human mammary epithelial tissue using th antibody. A-C: Strong TRAF4 staining is seen in the luminal epithelial cells of the interlobular terminal ducts (acini). However, there is absence of TRAF4 immunostaining in the myoepithelial cells along the basement membrane. B and C: higher magnifications of A. Data courtesy of Krajewska et al.
Immunoprecipitation: TRAF-4 Antibody [NB100-56177] - The NB100-56177 TRAF4 antibody specifically immunoprecipitates TRAF4. Top: TRAFs 2,3,4,5, and 6 were in vitro translated in the presence of 35S-methionine, and immunoprecipiated using the TRAF4 antibody. The TRAF4 antibody immunoprecipitated only TRAF4 and did not recognize TRAFs 2, 3, 5, or 6. A reticulocyte primed with an empty pcDNA plasmid was used as a negative control. Bottom: SDS-PAGE visualization of the in vitro translated TRAFs. Data courtesy of Krajewska et al.
(1 Publication)
Species: Hu, Mu, Rt, Bv, Ze
Applications: WB, IHC, IHC-P, IP
c-jun Antibody
(5B1)
NBP2-37576
Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Analysis using c-Jun mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.
Immunocytochemistry/Immunofluorescence: c-jun Antibody (5B1) [NBP2-37576] - Analysis of PC-2 cells using c-Jun mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: c-jun Antibody (5B1) [NBP2-37576] - Analysis of human rectum cancer tissues using c-Jun mouse mAb with DAB staining.
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P

TRANCE/TNFSF11/RANK L
462-TR
1 μg/lane of Recombinant Mouse TRANCE/TNFSF11/RANK L was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 36 kDa.
Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TR) induces osteoclast differentiation of the RAW 264.7 mouse monocyte/macrophage cell line. The ED50 for this effect is 5‑15 ng/mL in the presence of 2.5 μg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050)
(77 Publications)
Species: Mu
CD40 Ligand/TNFSF5
[HRP]
DCDL40
(39 Publications)
Species: Hu
Human, Mouse, Rat
RelA/NFkB p65 ELISA K ...
NBP2-29661
ELISA: Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric) [NBP2-29661] - Standard curve using the internal RelA standard.
ELISA: Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric) [NBP2-29661] - Nuclear translocation of NF-kB p65 with LPS in Jurkat cells. Jurkat cells, grown to a density of 2X10(6) cells/ml, were treated with LPS (15 ug/ml). The cells were harvested at various indicated time points, and cell lysates were tested in Western analysis using NB100-56712 and also in this ELISA Kit.
ELISA: Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric) [NBP2-29661] - Immunofluorescence indicates NF-kB p65 subunit was expressed exclusively in AA-G groups and AST-G cytoplasm. High glucose stimulation impact on TSMCs in NF-kB pathway. (A) NF-kB p65 protein subunit expression in TSMCs in each group. (B) NF-kB p65 subunit immunofluorescence. Each number denotes different subunits. Alexa 488 green served as secondary antibodies.Citation: Hara K, Hamada C, Wakabayashi K, Kanda R, Kaneko K, Horikoshi S, et al. (2017) Scavenging of reactive oxygen species by astaxanthin inhibits epithelial-mesenchymal transition in high glucose-stimulated mesothelial cells. PLoS ONE 12(9): e0184332. https://doi.org/10.1371/journal.pone.0184332
(85 Publications)
Species: Hu, Mu, Rt
CD30/TNFRSF8
Antibody (OTI1C6)
NBP2-46157
Western Blot: CD30/TNFRSF8 Antibody (1C6) [NBP2-46157] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF8.
Immunohistochemistry: CD30/TNFRSF8 Antibody (1C6) [NBP2-46157] - Analysis of Human endometrium tissue.
Flow Cytometry: CD30/TNFRSF8 Antibody (1C6) [NBP2-46157] - (Blue).(1:100)
(1 Review)
Species: Hu
Applications: WB, Flow, IHC, IHC-P
NIK/MAP3K14
Antibody
NBP2-23603
Western Blot: NIK/MAP3K14 Antibody [NBP2-23603] - Detection of MAP3K14 in U2OS cell lysate.
Immunocytochemistry/Immunofluorescence: NIK/MAP3K14 Antibody [NBP2-23603] - MAP3K14 antibody was tested in U2OS cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: NIK/MAP3K14 Antibody [NBP2-23603] - Analysis of MAP3K14 in mouse small intestine.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
HGK/MAP4K4
Antibody (4A5)
H00009448-M07
Western Blot: HGK/MAP4K4 Antibody (4A5) [H00009448-M07] - Analysis of MAP4K4 expression in K-562 (Cat # L009V1).
Immunohistochemistry-Paraffin: HGK/MAP4K4 Antibody (4A5) [H00009448-M07] - Analysis of monoclonal antibody to MAP4K4 on formalin-fixed paraffin-embedded human breast cancer. Antibody concentration 3 ug/ml
ELISA: HGK/MAP4K4 Antibody (4A5) [H00009448-M07] - Detection limit for recombinant GST tagged MAP4K4 is approximately 0.03ng/ml as a capture antibody.
(2 Publications)
Species: Hu, Bv, Pa
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
IKK alpha Antibody
(14A231) - BSA Free
NB100-56704
Simple Western: IKK alpha Antibody (14A231) - BSA Free [NB100-56704] - IKK alpha Antibody (14A231) [NB100-56704] - Image shows a specific band for IKK alpha in 0.5 mg/mL of Daudi (left) and U937 (right) lysate. This exeriment was performed under reducing conditions using the 12-230 kDa separation system.
Immunohistochemistry-Paraffin: IKK alpha Antibody (14A231) - BSA Free [NB100-56704] - Analysis of human IKK alpha in tumors of the three groups B (C-IKK alpha), E (Control), H (N-IKK alpha). Specific staining of human IKK alpha-using the NB100-56704 antibody. Scale bar: 70um. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/article/8792/text/) licensed under a CC-BY licence.
Western Blot: IKK alpha Antibody (14A231) - BSA Free [NB100-56704] - Western blot showing the increased expression of IKK-alpha in transgenic mice. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/8792), licensed under a CC-BY licence.
(79 Publications)
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
ASK1 Antibody
NB100-81788
Western Blot: ASK1 Antibody [NB100-81788] - Analysis of extracts from 293 and Hela cells using ASK1(Ab-966) antibody
Immunocytochemistry/Immunofluorescence: ASK1 Antibody [NB100-81788] - Staining of methanol-fixed Hela cells using ASK1(Ab-966) antibody
Immunohistochemistry-Paraffin: ASK1 Antibody [NB100-81788] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using ASK1 (Ab-966) antibody.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
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Publications for TRAF-5 Antibody (NB100-56178)(1)

We have publications tested in 2 applications: IHC-P, WB.


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Control Lysate(s)

TRAF-5 Overexpression Lysate
TRAF-5 Knockout HeLa Cell Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TRAF5
Entrez