PEA-15 Antibody

Images

 
Western blot shows lysates of A172 human glioblastoma cell line and human cortex tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse PEA‑15 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
PEA‑15 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Sheep Anti-Human/Mouse PEA‑15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5588) at 1 µg/mL overnight at ...read more
PED is inversely correlated to HNF4 alpha expression. (a) SNU-449 cells were co-transfected with 100 ng of pPED477 PED promoter-luciferase or pGL3 basic construct and treated with siRNA against HNF4 alpha or siRNA ...read more
PED confers resistance to sorafenib therapy. (a) HuH-7 and SNU-449 cells were transfected with siRNA against PED or siRNA control. Afterwards, HuH-7 and SNU-449 cells were treated with 10 μM and 20 μm respectively ...read more
PED is inversely correlated to HNF4 alpha expression. (a) SNU-449 cells were co-transfected with 100 ng of pPED477 PED promoter-luciferase or pGL3 basic construct and treated with siRNA against HNF4 alpha or siRNA ...read more
PED is inversely correlated to HNF4 alpha expression. (a) SNU-449 cells were co-transfected with 100 ng of pPED477 PED promoter-luciferase or pGL3 basic construct and treated with siRNA against HNF4 alpha or siRNA ...read more
PED is overexpressed in HCC samples. (a) PED expression levels in HCC samples and their matched non-tumoral (NT) counterpart measured by an mRNA gene expression microarray. Data are reported as probe intensity. (b) PED ...read more
PED modulates cell migration. (a) Western blot analysis of PED protein expression in 10 different HCC cell lines. beta -Actin was used as loading control. (b) HuH-7 and SNU-449 cells were transfected with PED-MYC or an ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

PEA-15 Antibody Summary

Immunogen
E. coli-derived recombinant human PEA-15
Ala2-Ala130
Accession # Q15121
Specificity
Detects human and mouse PEA-15 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
PEA15
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using AF5588.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PEA-15 Antibody

  • HMAT1
  • HMAT115kD
  • HUMMAT1H
  • MAT1H
  • PEA15
  • PEA-15
  • PED
  • PED15 kDa phosphoprotein enriched in astrocytes
  • phosphoprotein enriched in astrocytes 15
  • Phosphoprotein enriched in diabetes

Background

PEA-15 (Phosphoprotein Enriched in Astrocytes of 15 kDa) is a 15 kDa cytoplasmic protein that regulates cell proliferation and apoptosis. It is expressed in multiple cell types such as astrocytes; skeletal muscle and adipocytes. Human PEA-15 is 130 amino acids (aa) in length. It contains a death effector domain (aa 3‑81), plus two regulatory Ser phosphorylation sites. In a quiescent cell, it is either non-phosphorylated or constitutively phosphorylated at Ser116. Non-phosphorylated PEA-15 binds ERK, inhibiting cell proliferation. Phosphorylation at Ser116 promotes PEA-15 binding to FADD, blocking apoptosis. And activation-induced phosphorylation at Ser104 blocks PEA-15 binding to ERK, promoting cell proliferation. There is one potential alternate start site 21 aa upstream of the standard start site, and a second isoform that shows an Asn substitution for aa 36‑58. Full-length human and mouse PEA-15 show 99% aa identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PEA-15 Antibody (AF5588)(1)

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Bioinformatics

Gene Symbol PEA15
Uniprot