USP21 Antibody (3D10)

ELISA: USP21 Antibody (3D10) [H00027005-M03] - Detection limit for recombinant GST tagged USP21 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 3D10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

USP21 Antibody (3D10) Summary

• Immunogen: USP21 (NP_036607, 466 a.a. ~ 565 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FSASRGSIKKSSVGVDFPLQRLSLGDFASDKAGSPVYQLYALCNHSGSVHYGHYTALCRCQTGWHVYNDSRVSPVSENQVASSEGYVLFYQLMQEPPRCL
• Specificity: USP21 (3D10)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: USP21
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for USP21 Antibody (3D10)

• Deubiquitinating enzyme 21
• EC 3.1.2.15
• EC 3.4.19.12
• ESTM28
• MGC3394
• ubiquitin carboxyl-terminal hydrolase 21
• ubiquitin specific peptidase 21
• ubiquitin specific protease 21
• ubiquitin thioesterase 21
• Ubiquitin thiolesterase 21
• ubiquitin-specific processing protease 21
• ubiquitin-specific protease 16
• Ubiquitin-specific-processing protease 21
• USP16
• USP21
• USP23
• USP23NEDD8-specific protease
• W53272

Background

This gene encodes a member of the C19 peptidase family, also known as family 2 of ubiquitin carboxy-terminal hydrolases. The encoded protein cleaves ubiquitin from ubiquitinated proteins for recycling in intracellular protein degradation. The encoded protein is also able to release NEDD8, a ubiquitin-like protein, from NEDD8-conjugated proteins. This gene has been referred to as USP16 and USP23 but is now known as USP21. Alternatively spliced transcript variants have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for USP21 Antibody (H00027005-M03). (Showing 1 - 2 of 2 FAQ).

1. Can this product be used for ChIP (or IP)?
   • We have not yet tested this product in IP or ChIP. It has been validated in western blot and ELISA applications so far.
2. Has this product been tried on mouse?
   • We have not yet tested this product in mouse. The immunogen for this product does share 98% homology with the mouse USP21 protein, is it is likely to cross react.

Bioinformatics

• Gene Symbol: USP21
• Entrez:
  • 27005(Human)
• OMIM:
  • 604729(Human)
• Uniprot:
  • NP_036607()