Isopeptidase T/USP5 Antibody (OTI2E3)

Western Blot: USP5 Antibody (2E3) [NBP2-02648] Analysis of extracts (35ug) from 9 different cell lines by using anti-USP5 monoclonal antibody.

Immunohistochemistry-Paraffin: USP5 Antibody (2E3) [NBP2-02648] - Staining of paraffin-embedded Human thyroid tissue using anti-USP5 mouse monoclonal antibody.

Western Blot: USP5 Antibody (2E3) [NBP2-02648] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP5.

Immunohistochemistry-Paraffin: USP5 Antibody (2E3) [NBP2-02648] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-USP5 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: USP5 Antibody (2E3) [NBP2-02648] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-USP5 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: USP5 Antibody (2E3) [NBP2-02648] - Staining of paraffin-embedded Human colon tissue using anti-USP5 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: USP5 Antibody (2E3) [NBP2-02648] - Staining of paraffin-embedded Human Kidney tissue using anti-USP5 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: USP5 Antibody (2E3) [NBP2-02648] - Staining of paraffin-embedded Human lung tissue using anti-USP5 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: USP5 Antibody (2E3) [NBP2-02648] - Staining of paraffin-embedded Human pancreas tissue using anti-USP5 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, IHC
• Clone: OTI2E3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.45 mg/ml

Isopeptidase T/USP5 Antibody (OTI2E3) Summary

• Immunogen: Full length human recombinant protein of human USP5 (NP_003472) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: USP5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:1000-2000
• Theoretical MW: 93.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.45 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Isopeptidase T/USP5 Antibody (OTI2E3)

• Deubiquitinating enzyme 5
• EC 3.1.2.15
• EC 3.4.19.12
• Isopeptidase T
• IsoT
• ISOTubiquitin carboxyl-terminal hydrolase 5
• ubiquitin isopeptidase T
• ubiquitin specific peptidase 5 (isopeptidase T)
• ubiquitin specific protease 5 (isopeptidase T)
• ubiquitin thioesterase 5
• Ubiquitin thiolesterase 5
• ubiquitin-specific protease-5 (ubiquitin isopeptidase T)
• Ubiquitin-specific-processing protease 5
• USP5

Background

Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: USP5
• Entrez:
  • 8078(Human)