NEDP1/SENP8 Antibody

Western blot shows lysates of JAR human choriocarcinoma cell line, beta TC-6 mouse beta cell insulinoma cell line, and NRK-49F rat fibroblasts. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat NEDP1/SENP8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7760) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NEDP1/SENP8 at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

NEDP1/SENP8 Antibody Summary

• Immunogen: E. coli-derived recombinant human NEDP1/SENP8
  Met1-Lys212 (Thr207Ala)
  Accession # Q96LD8
• Specificity: Detects human, mouse, rat NEDP1/SENP8 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: SENP8
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NEDP1/SENP8 Antibody

• DEN1
• DEN1deneddylase-1
• deneddylase 1
• Deneddylase-1
• EC 3.4.22.-
• HsT17512
• NEDD8-specific protease 1
• NEDP1
• NEDP1NEDD8 specific-protease cysteine 2
• Protease, cysteine 2
• protease, cysteine, 2 (NEDD8 specific)
• PRSC2
• SENP8
• Sentrin/SUMO-specific protease SENP8
• sentrin-specific protease 8
• SUMO sentrin specific protease family member 8
• SUMO/sentrin specific peptidase family member 8
• SUMO/sentrin specific protease family member 8

Background

NEDP1 (NEDD8-specific protease 1; also DEN1/Deneddylase-1 Protease, cysteine 2 and SENP8/Sentrin-specific protease 8) is a 24-26 kDa cytoplasmic cysteine protease that belongs to the Ulp/peptidase C48 family of enzymes. It is widely expressed, and is involved in NEDD8-associated ubiquitination. NEDD8 is a 9 kDa polypeptide that covalently binds to, and activates cullin-1, a molecule associated with a ubiquitin ligase complex. NEDD8-activated cullin promotes the transfer of ubiquitin to a limited number of target substrates involved in the cell cycle. This has the effect of controlling the levels of cell cycle regulators within the cell. NEDP1 influences these activities in two ways; first, it converts preNEDD8 into its mature form, and second, it deconjugates NEDD8 from its bound substrates. Human NEDP1 is 212 amino acids (aa) in length. It possesses an acetylated initiating methionine plus a protease domain that spans aa 11-174. Over an equivalent sequence, full-length human NEDP1 shares 92% aa sequence identity with mouse NEDP1. The mouse NEDP1 molecule contains a nine aa extension at the C-terminus not shared by the human molecule.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SENP8
• Uniprot:
  • Q96LD8(Human)