Cullin 1 Antibody

Western Blot: Cullin 1 Antibody [NBP3-16092] - Western blot analysis of extracts of various cell lines, using Cullin 1 antibody (NBP3-16092) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunocytochemistry/Immunofluorescence: Cullin 1 Antibody [NBP3-16092] - Immunofluorescence analysis of HeLa cells using Cullin 1 antibody (NBP3-16092). Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Cullin 1 Antibody Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 435-593 of human Cullin 1 (NP_003583.2).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CUL1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:100
  • Immunohistochemistry 1:50 - 1:200
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% glycerol, pH7.3
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for Cullin 1 Antibody

• CUL1
• CUL-1
• Cullin 1
• cullin-1
• MGC149834
• MGC149835

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CUL1