![Western Blot: Cullin 1 Antibody [NBP3-16092] - Western blot analysis of extracts of various cell lines, using Cullin 1 antibody (NBP3-16092) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Cullin-1-Antibody-Western-Blot-NBP3-16092-img0002.jpg "Western Blot: Cullin 1 Antibody [NBP3-16092] - Western blot analysis of extracts of various cell lines, using Cullin 1 antibody (NBP3-16092) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 435-593 of human Cullin 1 (NP_003583.2). KNPEEAELEDTLNQVMVVFKYIEDKDVFQKFYAKMLAKRLVHQNSASDDAEASMISKLKQACGFEYTSKLQRMFQDIGVSKDLNEQFKKHLTNSEPLDLDFSIQVLSSGSWPFQQSCTFALPSELERSYQRFTAFYASRHSGRKLTWLYQLSKGELVTN |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CUL1 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 50% glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Cullin 1 Antibody (NBP3-16092)Find related products by research area.
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| Gene Symbol | CUL1 |
![Immunocytochemistry/Immunofluorescence: Cullin 1 Antibody [NBP3-16092] - Immunofluorescence analysis of HeLa cells using Cullin 1 antibody (NBP3-16092). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cullin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-16092-img0001.jpg "Immunocytochemistry/Immunofluorescence: Cullin 1 Antibody [NBP3-16092] - Immunofluorescence analysis of HeLa cells using Cullin 1 antibody (NBP3-16092). Blue: DAPI for nuclear staining.")
![IHC-P- Cullin 1 Antibody [NBP3-16092] - Human colon using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/antibody/nbp3-16092_rabbit-cullin-1-pab-immunohistochemistry-paraffin-10820237323..jpg "IHC-P- Cullin 1 Antibody [NBP3-16092] - Human colon using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.")
![IHC-P- Cullin 1 Antibody [NBP3-16092] - Human lung cancer using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/antibody/nbp3-16092_rabbit-cullin-1-pab-immunohistochemistry-paraffin-10820237454..jpg "IHC-P- Cullin 1 Antibody [NBP3-16092] - Human lung cancer using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.")
![IHC-P- Cullin 1 Antibody [NBP3-16092] -Rat lung using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/antibody/nbp3-16092_rabbit-cullin-1-pab-immunohistochemistry-paraffin-10820237622..jpg "IHC-P- Cullin 1 Antibody [NBP3-16092] -Rat lung using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.")
![Immunohistochemistry: Cullin 1 Antibody - Azide and BSA Free [NBP3-16092] - Immunohistochemistry analysis of paraffin-embedded Human colon using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16092_rabbit-cullin-1-pab-522025126535.jpg "Immunohistochemistry: Cullin 1 Antibody - Azide and BSA Free [NBP3-16092] - Immunohistochemistry analysis of paraffin-embedded Human colon using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunocytochemistry/ Immunofluorescence: Cullin 1 Antibody - Azide and BSA Free [NBP3-16092] - Immunofluorescence analysis of HeLa cells using Cullin 1 Rabbit pAb . Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16092_rabbit-cullin-1-pab-5220251136126.jpg "Immunocytochemistry/ Immunofluorescence: Cullin 1 Antibody - Azide and BSA Free [NBP3-16092] - Immunofluorescence analysis of HeLa cells using Cullin 1 Rabbit pAb . Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunohistochemistry: Cullin 1 Antibody - Azide and BSA Free [NBP3-16092] - Immunohistochemistry analysis of paraffin-embedded Rat lung using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16092_rabbit-cullin-1-pab-52202511411473.jpg "Immunohistochemistry: Cullin 1 Antibody - Azide and BSA Free [NBP3-16092] - Immunohistochemistry analysis of paraffin-embedded Rat lung using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: Cullin 1 Antibody - Azide and BSA Free [NBP3-16092] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16092_rabbit-cullin-1-pab-52202511411496.jpg "Immunohistochemistry: Cullin 1 Antibody - Azide and BSA Free [NBP3-16092] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: Cullin 1 Antibody - Azide and BSA Free [Cullin 1] - Immunohistochemistry analysis of paraffin-embedded Human colon using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16092_rabbit-cullin-1-pab-52202517251944.jpg "Immunohistochemistry: Cullin 1 Antibody - Azide and BSA Free [Cullin 1] - Immunohistochemistry analysis of paraffin-embedded Human colon using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: Cullin 1 Antibody - Azide and BSA Free [Cullin 1] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16092_rabbit-cullin-1-pab-52202517162326.jpg "Immunohistochemistry: Cullin 1 Antibody - Azide and BSA Free [Cullin 1] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: Cullin 1 Antibody - Azide and BSA Free [Cullin 1] - Immunohistochemistry analysis of paraffin-embedded Rat lung using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16092_rabbit-cullin-1-pab-5220251717288.jpg "Immunohistochemistry: Cullin 1 Antibody - Azide and BSA Free [Cullin 1] - Immunohistochemistry analysis of paraffin-embedded Rat lung using Cullin 1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")