ULK3 Antibody (5Z7Z6)

Immunocytochemistry/Immunofluorescence: ULK3 Antibody (5Z7Z6) [NBP3-16622] - Immunofluorescence analysis of C6 cells using ULK3 Rabbit mAb (NBP3-16622) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunocytochemistry/Immunofluorescence: ULK3 Antibody (5Z7Z6) [NBP3-16622] - Immunofluorescence analysis of NIH-3T3 cells using ULK3 Rabbit mAb (NBP3-16622) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: ULK3 Antibody (5Z7Z6) [NBP3-16622] - Confocal imaging of C2C12 cells using ULK3 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Western Blot: ULK3 Antibody (5Z7Z6) [NBP3-16622] - Western blot analysis of lysates from 293T cells using ULK3 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 10s.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: 5Z7Z6
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

ULK3 Antibody (5Z7Z6) Summary

• Description: Novus Biologicals Rabbit ULK3 Antibody (5Z7Z6) (NBP3-16622) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 373-472 of human ULK3 (Q6PHR2). KPRLLAALEVASAAMAKEEAAGGEQDALDLYQHSLGELLLLLAAEPPGRRRELLHTEVQNLMARAEYLKEQVKMRESRWEADTLDKEGLSESVRSSCTLQ
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ULK3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for ULK3 Antibody (5Z7Z6)

• DKFZP434C131
• EC 2.7.11.1
• FLJ90566
• serine/threonine-protein kinase ULK3
• unc-51-like kinase 3 (C. elegans)
• Unc-51-like kinase 3

Background

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ULK3