FGF-5 Antibody

Images

 
FGF‑5 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human FGF‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑237‑NA) at 10 µg/mL overnight at 4 °C. ...read more
Recombinant Human FGF‑5 (Catalog # 237-F5) stimulates proliferation in the the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

FGF-5 Antibody Summary

Immunogen
E. coli-derived recombinant human FGF-5
Glu23-Gly268 (Lys238Asn, Pro245Ser)
Accession # Q8NF90
Specificity
Detects human FGF‑5 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) FGF‑4, rhFGF-6, rhFGF-7, recombinant mouse FGF-8b, rhFGF-9, rhFGF acidic, and rhFGF basic is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FGF5
Purity
Immunogen affinity purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.2-0.8 ug/mL
Publications
Read Publications using
AF-237-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGF-5 Antibody

  • FGF5
  • FGF-5
  • fibroblast growth factor 5
  • HBGF-5
  • Heparin-binding growth factor 5
  • Smag-82

Background

The FGF family is comprised of at least seven polypeptides that are potent regulators of cell proliferation, differentiation and function. All FGFs have two conserved cysteine residues and share 30-50% sequence homology at the amino acid level. FGF-5 was originally identified as a transforming gene by the NIH-3T3 focus formation assay using DNA derived from human tumors. FGF-5 cDNA encodes a 267 amino acid residue protein with a putative 22 amino acid residue signal peptide. The murine homologue of FGF-5 was cloned and found to be 84% homologous to the human protein at the amino acid sequence level. Human and murine FGF-5 exhibit cross species activity.

In vitro, recombinant human FGF-5 is a mitogen for Balb/3T3 fibroblasts and bovine heart endothelial cells. FGF-5 was also reported to be a major muscle-derived survival factor for cultured spinal motoneurons. In vivo, FGF-5 is suggested to play important roles in both embryology and neurobiology. Developmentally, FGF-5 mRNA is initially found in the embryoblast followed by the lateral somatic mesoderm, where it may play a role in angiogenesis, plus the myotomes cranial to the tail region, where it may delay terminal myoblast differentiation during cell migration. FGF-5 continues to impact muscle post-natally where it is believed to function as a target-derived neurotrophic factor of skeletal muscle. In the nervous system, FGF-5 has been most often identified in neurons associated with the limbic system, notably in neurons of the olfactory bulb and pyramidal cells of the hippocampus. Hippocampal FGF-5 is suggested to serve as a neurotrophic and differentiative factor for cholinergic and serotonergic neurons projecting to this region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FGF-5 Antibody (AF-237-NA)(3)

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Bioinformatics

Gene Symbol FGF5
Uniprot