UCP3 Antibody - BSA Free

Immunohistochemistry-Paraffin: UCP3 Antibody [NBP2-58840] - Staining of human skin shows low expression as expected.

Immunohistochemistry-Paraffin: UCP3 Antibody [NBP2-58840] - Staining of human skeletal muscle shows high expression.

Immunohistochemistry-Paraffin: UCP3 Antibody [NBP2-58840] - Staining in human skeletal muscle and skin tissues using anti-UCP3 antibody. Corresponding UCP3 RNA-seq data are presented for the same tissues.

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

UCP3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit UCP3 Antibody - BSA Free (NBP2-58840) is a polyclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: AEVVTYDILKEKLLDYHLLTDNFPCHFV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UCP3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  UCP3 Recombinant Protein Antigen (NBP2-58840PEP)

Reactivity Notes

Mouse 82%, Rat 86%

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for UCP3 Antibody - BSA Free

• mitochondrial uncoupling protein 3
• SLC25A9Solute carrier family 25 member 9
• UCP 3
• uncoupling protein 3 (mitochondrial, proton carrier)
• Uncoupling protein-3

Background

The uncoupling proteins (UCPs) are found in the inner mitochondrial membranes and belong to a family of mitochondrial anion transport proteins that include the phosphate and oxologutarate carriers and the ADP/ATP translocator. Three UCP subtypes have been identified and cloned. UCP 1 is abundantly expressed in brown adipose tissue of mammals. UCP 1 allows proton re-entry into the mitochondrial matrix without involving the F0-F1 ATPase which produces heat instead of ATP. It is thought that UCP 2 and 3 also dissipate the mitochondrial proton gradient produced by the respiratory chain in cells and tissues within which they are expressed. Studies have shown UCP 2 to be expressed by a variety of tissues. UCP 3 is expressed predominantly in brown adipose tissue, skeletal muscle, at lower levels in heart and smooth muscle, and is absent in liver and kidney.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UCP3