Western Blot: UCP1 Antibody [NBP2-20796] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with UCP1 antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: UCP1 Antibody [NBP2-20796] - UCP1 antibody detects UCP1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brown adipocyte. UCP1 stained by UCP1 antibody ...read more
Immunohistochemistry-Paraffin: UCP1 Antibody [NBP2-20796] - Immunohistochemical analysis of paraffin-embedded Colon carcinoma, using antibody at 1:250 dilution.
Use in KO reported in scientific publication (PMID: 32713230).
33 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NBP2-20796 in the following applications:
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%), Rabbit (86%), Sheep (83%), Bovine (83%).
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS (pH 7), 20% Glycerol
0.025% Proclin 300
Immunogen affinity purified
Alternate Names for UCP1 Antibody
SLC25A7mitochondrial brown fat uncoupling protein 1
Solute carrier family 25 member 7
uncoupling protein 1 (mitochondrial, proton carrier)
Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat. [provided by RefSeq]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for UCP1 Antibody (NBP2-20796). (Showing 1 - 1 of 1 FAQs).
How specific are the UCP1 antibodies to UCP1 than to UCP2 and 3?
None of our UCP1 antibodies have been tested for cross-reactivity with UCP2 or UCP3, so we cannot guarantee that these antibodies will not bind to the other UCP proteins. However, we can determine the likelihood of this cross-reactivity by running sequence alignments using the immunogen sequences. NB100-2828 is the most likely to be specific for UCP1: its short immunogen sequence is shown on its datasheet, and when I run a UniProt BLAST sequence comparison for human proteins it detects a match with UCP1 (100%), but not UCP2 or UCP3. When I use MultAlin to align the immunogen sequence with the UCP2 and UCP3 protein sequences, it shows only low similarity. For the antibodies with product codes beginning H00007350, again the immunogen sequence is shown on the datasheets for the products. In this case, the immunogen sequence (shared between them) is a little longer. When I run the UniProt Blast, the results are 100% UCP1, 66% UCP3 and 58% UCP2. For NBP2-20796, the exact immunogen sequence is not available, but I have run the UniProt Blast on the amino acid range given, and the results are 100% UCP1, 59% UCP2 and 58% UCP3. Percentages in the 50s-60s suggest that the antibody probably won't cross-react, but may.
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