UCH-L1/PGP9.5 Antibody (CL3210) - BSA Free

Western Blot: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue

Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Staining of U-251 cells using the Anti-UCHL1 monoclonal antibody, showing specific staining in the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Analysis of human fallopian tube shows absence of immunoreactivity (negative control).

Western Blot: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human cell line U-251 AAb

Western Blot: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue

Immunohistochemistry: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Analysis of rat cerebral cortex shows moderate immunoreactivity in neurons.

Immunohistochemistry: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Analysis of mouse trigeminal ganglion shows strong immunoreactivity in primary sensory neurons.

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Analysis of human cerebellum shows moderate positivity in Purkinje cells.

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Analysis of human prostate shows staining in peripheral nerve fibers.

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Analysis of human duodenum shows staining in peripheral nerve fibers.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: CL3210
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

UCH-L1/PGP9.5 Antibody (CL3210) - BSA Free Summary

• Immunogen: This antibody was developed using a recombinant protein derived from P09936, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: UCHL1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:5000 - 1:10000
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, Fixation/Permeabilization: PFA/Triton X-100
• Control Peptide:

  UCH-L1/PGP9.5 Recombinant Protein Antigen (NBP2-46621PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for UCH-L1/PGP9.5 Antibody (CL3210) - BSA Free

• EC 3.4.19.12
• EC 6.-
• Neuron cytoplasmic protein 9.5
• PARK5
• PGP 9.5
• PGP9.5
• PGP9.5Uch-L1
• PGP95
• ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
• ubiquitin carboxyl-terminal hydrolase isozyme L1
• ubiquitin C-terminal hydrolase
• Ubiquitin thioesterase L1
• UCHL1
• UCH-L1

Background

Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) is a soluble cytoplasmic protein found in neurons and in cells of the diffuse neuroendocrine system, and their respective tumors. Therefore, UCH-L1 has been widely used as a peripheral nerve fiber marker. UCHL-1 functions as a tissue-specific ubiquitin carboxyl terminal hydrolase isoenzyme involved in the processing of both ubiquitin precursors and ubiquitinated proteins.

UCH L1 is down-regulated in brains from Parkinson disease and Alzheimer disease patients, and certian site specific mutations in the UCHL1 gene can either increase or decrese the risk of Parkinson's and/or Alzheimer's neurodegenerative diseases.

Human UCHL 1 and the closely related UCHL3 protein have one of the most complicated knot structures ever discovered, with five knot crossings. This knot structure is expected to help the protein resist degradation in the proteasome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UCHL1
• Entrez:
  • 7345(Human)
• Uniprot:
  • P09936(Human)