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Ubiquitin B Antibody (UBB/1748)

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Western Blot: Ubiquitin B Antibody (UBB/1748) [NBP3-07163] - Western Blot Analysis of human Raji cell lysate using Ubiquitin B-Monospecific Mouse Monoclonal Antibody (UBB/1748).
Western Blot: Ubiquitin B Antibody (UBB/1748) [NBP3-07163] - Western Blot Analysis of human Raji cell lysate using Ubiquitin B-Monospecific Mouse Monoclonal Antibody (UBB/1748).
Immunohistochemistry-Paraffin: Ubiquitin B Antibody (UBB/1748) [NBP3-07163] - Formalin-fixed, paraffin-embedded human Brain stained with Ubiquitin B-Monospecific Mouse Monoclonal Antibody (UBB/1748).
Flow Cytometry: Ubiquitin B Antibody (UBB/1748) [NBP3-07163] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using Ubiquitin B-Monospecific Mouse Monoclonal Antibody (UBB/1748) followed by goat anti- Mouse IgG-CF488 ...read more
Immunohistochemistry-Paraffin: Ubiquitin B Antibody (UBB/1748) [NBP3-07163] - Formalin-fixed, paraffin-embedded human Brain stained with Ubiquitin B-Monospecific Mouse Monoclonal Antibody (UBB/1748).
Protein Array: Ubiquitin B Antibody (UBB/1748) [NBP3-07163] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Ubiquitin B antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, PA
Clone
UBB/1748
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-08905AF350
Alexa Fluor 405
NBP3-08905AF405
Alexa Fluor 488
NBP3-08905AF488
Alexa Fluor 532
NBP3-08905AF532
Alexa Fluor 594
NBP3-08905AF594
Alexa Fluor 647
NBP3-08905AF647
Alexa Fluor 700
NBP3-08905AF700
Alexa Fluor 750
NBP3-08905AF750
Allophycocyanin
NBP3-08905APC
Biotin
NBP3-08905B
DyLight 350
NBP3-08905UV
DyLight 405
NBP3-08905V
DyLight 488
NBP3-08905G
DyLight 550
NBP3-08905R
DyLight 594
NBP3-08905DL594
DyLight 650
NBP3-08905C
DyLight 680
NBP3-08905FR
DyLight 755
NBP3-08905IR
FITC
NBP3-08905F
HRP
NBP3-08905H
Janelia Fluor 549
NBP3-08905JF549
Janelia Fluor 646
NBP3-08905JF646
PE
NBP3-08905PE
PerCP
NBP3-08905PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP3-08905
100% Guarantee on every product

Ubiquitin B Antibody (UBB/1748) Summary

Immunogen
Recombinant fragment of human Ubiquitin B protein (around aa 1-119) (exact sequence is proprietary)
Localization
Cell Surface, Cytoplasmic and Nuclear
Marker
Autophagy Marker
Isotype
IgG2c Kappa
Clonality
Monoclonal
Host
Mouse
Gene
UBB
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08905)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for Ubiquitin B Antibody (UBB/1748)

  • FLJ25987
  • MGC8385
  • polyubiquitin B
  • polyubiquitin-B
  • RPS27A
  • UBA52
  • UBC
  • ubiquitin B
  • Ubiquitin+1

Background

ubiquitin B encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1D4) [NB300-221] - Analysis of of HeLa cells stained with GAPDH antibody, dilution 1:100 (Green). DAPI staining of nuclear DNA (Blue). The GAPDHantibody produces strong cytoplasmic staining of healthy cells.
Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1D4) [NB300-221] - SH-SY5Y cells stained with GAPDH antibody NB300-221 (green). Nuclear DNA is stained with Hoechst dye (blue).
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Species: Av, Bv, Ca, Ch, ChHa, Dr, Eq, Fe, Ha, Hu, Mu, Ma-Op, Po, Pm, Rb, Rt, Sh, Ze
Applications: ChIP, ICC/IF, IHC, IHC-Fr, IP, KD, Simple Western, Single-Cell Western, WB
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Western Blot: Actin Gamma 1 Antibody [NB600-533] - Detection of human and mouse Cytoskeletal Actin by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cytoskeletal Actin antibody NB600-533 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Western Blot: Actin Gamma 1 Antibody [NB600-533] - Whole cell lysate from HeLa (1, 2.5, 5, 15 and 50 ug) and mouse NIH3T3 cells (50 ug), probed with diluted at 0.04 ug/ml.
Western Blot: Actin Gamma 1 Antibody [NB600-533] - Knockdown of DLK in differentiated Neuro-2a cells. Neuro-2a cells were infected with an empty lentiviral vector (pLKO.1) or with lentivirus expressing mouse DLK shRNAs (sh73 and sh69). After infection and selection with puromycin, cells were subjected to differentiation for 24 h before being processed for total RNA extraction and whole-cell extracts. Representative Western blots showing levels of DLK, phospho-JNK (p-JNK), total JNK, phospho-c-Jun (p-c-Jun) and actin in infected Neuro-2a cells. Image collected and cropped by CiteAb from the following publication (http://neuraldevelopment.biomedcentral.com/articles/10.1186/s13064-016-0068-8), licensed under a CC-BY licence.
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UBE2K/E2-25K
Antibody (701316)
MAB6609
Western blot shows lysates of human brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human UBE2K/E2‑25K Monoclonal Antibody (Catalog # MAB6609) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for UBE2K/E2‑25K at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
UBE2K/E2‑25K was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Mouse Anti-Human UBE2K/E2‑25K Monoclonal Antibody (Catalog # MAB6609) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter­stained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Species: Hu, Mu
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beta
2-Microglobulin Antib ...
NB500-317
Western Blot: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Analysis using the FITC conjugate of NB500-317. Detection (non-reducing conditions) of whole cell lysate of various cell lines Lane 1: RAJI human Burkitt lymphoma cell line Lane 2: EL4 mouse lymphoblastic lymphoma cell line Lane 3: U937 human histiocytic lymphoma cell line.
Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Staining of beta-2-microglobulin in human peripheral blood with anti-beta-2-microglobulin (B2M-01) biotin.
ELISA: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Analysis of HLA-G and beta2-microglobulin in supernatant of HLA-G transfectants with anti-beta2-microglobulin (B2M-01) HRP as the detection antibody. Image using the HRP format of this antibody.
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Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, IP, RIA, WB
DBT Antibody
NBP1-89522
Immunocytochemistry/Immunofluorescence: DBT Antibody [NBP1-89522] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining of human pancreas shows weak to moderate cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining of human testis shows weak to moderate cytoplasmic positivity in Leydig cells.
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HPRT Antibody
NBP1-33527
Western Blot: HPRT Antibody [NBP1-33527] - Wild-type (WT) and HPRT knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: HPRT Antibody [NBP1-33527] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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NB600-501
Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - HEK293T cells transfected with Niaph virus Matrix Protein, expression 24h later. This image was submitted via customer review.
Simple Western: beta-Actin Antibody (AC-15) [NB600-501] - Analysis using the HRP conjugate of NB600-501. Electropherogram image(s) of corresponding Simple Western lane view. Beta-Actin antibody was used at 1:500 dilution on Hela, MCF-7, SH-SY5Y, & Jurkat lysate(s).
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NBP2-33600
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Immunohistochemistry-Paraffin: HMBS Antibody [NBP2-33600] - Staining in human bone marrow and cerebral cortex tissues using anti-HMBS antibody. Corresponding HMBS RNA-seq data are presented for the same tissues.
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Antibody
NBP1-76593
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Western Blot: UBE2N/Ubc13 Antibody [NBP1-76593] - Human small intestine cell lysates with Ube2N antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.
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Butyrylcholinesterase/ ...
AF9024
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Butyrylcholinesterase/BCHE was detected in perfusion fixed frozen sections of mouse brain using Goat Anti-Mouse/Rat Butyrylcholinesterase/BCHE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9024) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit  (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to glial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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MASA Antibody
NBP2-13961
Immunohistochemistry-Paraffin: MASA Antibody [NBP2-13961] - Staining of human cerebral cortex shows high expression.
Immunohistochemistry-Paraffin: MASA Antibody [NBP2-13961] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: MASA Antibody [NBP2-13961] - Staining in human cerebral cortex and pancreas tissues using anti-ENOPH1 antibody. Corresponding ENOPH1 RNA-seq data are presented for the same tissues.
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14-3-3 zeta
Antibody (818515) [Un ...
MAB2669
Simple Western lane view shows lysates of SH‑SY5Y human neuroblastoma cell line, loaded at 0.5 mg/mL. A specific band was detected for 14‑3‑3 zeta at approximately 34 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human 14‑3‑3 zeta Monoclonal Antibody (Catalog # MAB2669). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line,SK‑Mel‑28 human malignant melanoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and C6 rat glioma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human14‑3‑3 zeta  Monoclonal Antibody (Catalog # MAB2669) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for14‑3‑3 zeta at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
14‑3‑3 zeta  was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human 14‑3‑3 zeta Monoclonal Antibody (Catalog # MAB2669) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter­stained with hematoxylin (blue). Specific staining was localized to nuclei and plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Elastase Antibody
(CELA3B/1257) - Azide ...
NBP2-54301
Immunohistochemistry-Paraffin: Elastase Antibody (CELA3B/1257) - Azide and BSA Free [NBP2-54301] - Formalin-fixed, paraffin-embedded Human Pancreas stained with CELA3B Monoclonal Antibody (CELA3B/1257)
Immunohistochemistry-Paraffin: Elastase Antibody (CELA3B/1257) - Azide and BSA Free [NBP2-54301] - Formalin-fixed, paraffin-embedded Rat Pancreas stained with CELA3B Monoclonal Antibody (CELA3B/1257).
Immunohistochemistry-Paraffin: Elastase Antibody (CELA3B/1257) - Azide and BSA Free [NBP2-54301] - Formalin-fixed, paraffin-embedded Mouse Pancreas stained with CELA3B Monoclonal Antibody (CELA3B/1257).
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Applications: CyTOF-ready, ELISA, IHC, IHC-P, WB
SDHA Antibody
(GT20710)
NBP3-13522
Western Blot: SDHA Antibody (GT20710) [NBP3-13522] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (NBP3-13522) diluted at 1:3000.
Western Blot: SDHA Antibody (GT20710) [NBP3-13522] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (NBP3-13522) diluted at 1:3000.
Western Blot: SDHA Antibody (GT20710) [NBP3-13522] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (NBP3-13522) diluted at 1:3000.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
TATA binding
protein TBP Antibody
NBP3-13174
Western Blot: TATA binding protein TBP Antibody [NBP3-13174] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TATA binding protein TBP antibody (NBP3-13174) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: TATA binding protein TBP Antibody [NBP3-13174] - TATA binding protein TBP antibody detects TATA binding protein TBP protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. TATA binding protein TBP stained by TATA binding protein TBP antibody (NBP3-13174) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: TATA binding protein TBP Antibody [NBP3-13174] - TATA binding protein TBP antibody detects TATA binding protein TBP protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. TATA binding protein TBP stained by TATA binding protein TBP antibody (NBP3-13174) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
UbcH5b/UBE2D2
E2-622
UbcH5b/UBE2D2
(37 Publications)
Species: Hu
Applications: EnzAct
EIF3B Antibody
NB100-93302
Immunohistochemistry-Paraffin: EIF3B Antibody [NB100-93302] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit antiEIF3S9/eIF3B used at a dilution of 1:200 (1ug/ml). Detection: DAB
Western Blot: EIF3B Antibody [NB100-93302] - Whole cell lysate (15 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eIF3B antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunoprecipitation: EIF3B Antibody [NB100-93302] - Detection of human eIF3B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eIF3B antibody NB100-93302 used for IP at 3 ug per reaction. eIF3B was also immunoprecipitated by another rabbit anti-eIF3B antibody. For blotting immunoprecipitated eIF3B, NB100-93302 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
(2 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
beta Amyloid
Antibody (MOAB-2) - B ...
NBP2-13075
Immunohistochemistry-Frozen: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Mouse brain (cerebral cortex). Red: MOAB-2 antibody staining, Blue: DAPI. Zeiss LSM800, 40x. Image from verified customer review.
Immunohistochemistry: beta Amyloid Antibody (MOAB-2) - BSA Free [NBP2-13075] - A plaque load in neocortex and hippocampus in APP/PS1 mice exposed to differential housing. Representative 10x MOAB-2 staining in hippocampus of APP/PS1 mice for each housing group. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28584278/) licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Immunofluorescent detection of beta Amyloid with MOAB-2 in the subiculim of 1-, 2- and 4- month old 5xFAD mice. Scale bar 20 um (from PMID: 22423893).
(2 Reviews)
(50 Publications)
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Ubiquitin B
Antibody (UBB/1748)
NBP3-07163
Ubiquitin B Antibody (UBB/1748)
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, PA

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Bioinformatics

Gene Symbol UBB
Uniprot