Ubiquitin B Antibody (UBB/1748)

Images

 
Western Blot: Ubiquitin B Antibody (UBB/1748) [NBP3-07163] - Western Blot Analysis of human Raji cell lysate using Ubiquitin B-Monospecific Mouse Monoclonal Antibody (UBB/1748).
Immunohistochemistry-Paraffin: Ubiquitin B Antibody (UBB/1748) [NBP3-07163] - Formalin-fixed, paraffin-embedded human Brain stained with Ubiquitin B-Monospecific Mouse Monoclonal Antibody (UBB/1748).
Flow Cytometry: Ubiquitin B Antibody (UBB/1748) [NBP3-07163] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using Ubiquitin B-Monospecific Mouse Monoclonal Antibody (UBB/1748) followed by goat anti- Mouse IgG-CF488 ...read more
Immunohistochemistry-Paraffin: Ubiquitin B Antibody (UBB/1748) [NBP3-07163] - Formalin-fixed, paraffin-embedded human Brain stained with Ubiquitin B-Monospecific Mouse Monoclonal Antibody (UBB/1748).
Protein Array: Ubiquitin B Antibody (UBB/1748) [NBP3-07163] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Ubiquitin B antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, PA
Clone
UBB/1748
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Ubiquitin B Antibody (UBB/1748) Summary

Immunogen
Recombinant fragment of human Ubiquitin B protein (around aa 1-119) (exact sequence is proprietary)
Localization
Cell Surface, Cytoplasmic and Nuclear
Marker
Autophagy Marker
Isotype
IgG2c Kappa
Clonality
Monoclonal
Host
Mouse
Gene
UBB
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08905)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for Ubiquitin B Antibody (UBB/1748)

  • FLJ25987
  • MGC8385
  • polyubiquitin B
  • polyubiquitin-B
  • RPS27A
  • UBA52
  • UBC
  • ubiquitin B
  • Ubiquitin+1

Background

ubiquitin B encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB300-221
Species: Av, Bv, Ca, Ch, ChHa, Dr, Eq, Fe, Ha, Hu, Mu, Ma-Op, Po, Pm, Rb, Rt, Sh, Ze
Applications: ChIP, ICC/IF, IHC, IHC-Fr, IP, KD, Simple Western, Single-Cell Western, WB
NB600-533
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IP, Simple Western, WB
MAB6609
Species: Hu, Mu
Applications: IHC, WB
NB500-317
Species: Bv(-), Ca(-), Ch(-), Hu, Mu(-), Rb(-)
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, IP, RIA, WB
NBP1-89522
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-33527
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB600-501
Species: Bv, Ca, Ch, Dr(-), Fe, Fi, Gp, Ha, Hu, Le, Ma, Mu, Po, Pm, Rb, Rt, Sh, Sq, Tr, Ze
Applications: ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO, Simple Western, WB
NBP2-33600
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NB100-56360
Species: Hu
Applications: WB
NBP1-76593
Species: Hu, Mu, Rt
Applications: ELISA, IF, IHC, IHC-P, WB
AF9024
Species: Mu, Rt
Applications: IHC, WB
NBP2-13961
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
MAB2669
Species: Hu
Applications: IHC, Simple Western, WB
NBP2-54301
Species: Hu, Mu, Rt
Applications: CyTOF-ready, ELISA, IHC, IHC-P, WB
NBP3-13522
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP3-13174
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
E2-622
Species: Hu
Applications: EnzAct
NB100-93302
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP2-13075
Species: Ba, Hu, Pm, Mu, Rt
Applications: DB, ELISA, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, WB
NBP3-07163
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, PA

Publications for Ubiquitin B Antibody (NBP3-07163) (0)

There are no publications for Ubiquitin B Antibody (NBP3-07163).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Ubiquitin B Antibody (NBP3-07163) (0)

There are no reviews for Ubiquitin B Antibody (NBP3-07163). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Ubiquitin B Antibody (NBP3-07163) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Ubiquitin B Products

Bioinformatics Tool for Ubiquitin B Antibody (NBP3-07163)

Discover related pathways, diseases and genes to Ubiquitin B Antibody (NBP3-07163). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Ubiquitin B Antibody (NBP3-07163)

Discover more about diseases related to Ubiquitin B Antibody (NBP3-07163).
 

Pathways for Ubiquitin B Antibody (NBP3-07163)

View related products by pathway.

PTMs for Ubiquitin B Antibody (NBP3-07163)

Learn more about PTMs related to Ubiquitin B Antibody (NBP3-07163).
 

Research Areas for Ubiquitin B Antibody (NBP3-07163)

Find related products by research area.

Blogs on Ubiquitin B

There are no specific blogs for Ubiquitin B, but you can read our latest blog posts.
Download our IHC Handbook

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Ubiquitin B Antibody (UBB/1748) and receive a gift card or discount.

Bioinformatics

Gene Symbol UBB
Uniprot