Elastase Antibody (CELA3B/1257) - Azide and BSA Free

Images

 
Immunohistochemistry-Paraffin: Elastase Antibody (CELA3B/1257) [NBP2-54301] - Formalin-fixed, paraffin-embedded Mouse Pancreas stained with CELA3B Monoclonal Antibody (CELA3B/1257).
Immunohistochemistry-Paraffin: Elastase Antibody (CELA3B/1257) [NBP2-54301] - Formalin-fixed, paraffin-embedded Human Pancreas stained with CELA3B Monoclonal Antibody (CELA3B/1257)
Immunohistochemistry-Paraffin: Elastase Antibody (CELA3B/1257) [NBP2-54301] - Formalin-fixed, paraffin-embedded Rat Pancreas stained with CELA3B Monoclonal Antibody (CELA3B/1257).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, CyTOF-ready
Clone
CELA3B/1257
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Elastase Antibody (CELA3B/1257) - Azide and BSA Free Summary

Immunogen
Recombinant human Elastase protein fragment (around aa 82-238) (exact sequence is proprietary)
Localization
Cytoplasmic
Marker
Pancreatic Function Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CELA3B
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • ELISA 2 - 4 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • CyTOF-ready
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Notes

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A or G prepared in 10 mM PBS WITHOUT BSA & azide.

Alternate Names for Elastase Antibody (CELA3B/1257) - Azide and BSA Free

  • CBPP
  • cholesterol-binding pancreatic protease
  • chymotrypsin-like elastase family member 3B
  • chymotrypsin-like elastase family, member 3B
  • E1
  • EC 3.4.21
  • EC 3.4.21.70
  • EL-1
  • ELA3B
  • elastase 1
  • elastase 3B, pancreatic
  • Elastase IIIB
  • elastase-3B
  • fecal elastase 1
  • pancreatic elastase 1
  • pancreatic endopeptidase E
  • Protease E
  • proteinase E

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CELA3B