UBE2N/Ubc13 Antibody - BSA Free

Western Blot: UBE2N/Ubc13 Antibody [NBP1-76593] - Human small intestine cell lysates with Ube2N antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.

Immunohistochemistry: UBE2N/Ubc13 Antibody [NBP1-76593] - Analysis of UBC13 in mouse thymus tissue with UBC13 antibody at 2.5 ug/ml.

Immunohistochemistry-Paraffin: UBE2N/Ubc13 Antibody [NBP1-76593] - Mouse thymus tissue with Ube2N antibody at 2 ug/ml.

Immunocytochemistry/Immunofluorescence: UBE2N/Ubc13 Antibody [NBP1-76593] - Mouse Thymus cells with UBC13 antibody at 10 ug/mL.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

UBE2N/Ubc13 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human UBC13. The immunogen is located within the last 50 amino acids of UBC13. Amino Acid Squence: NPDDPLANDVAEQWK
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBE2N
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 10 ug/mL
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 0.5-2 ug/ml
• Control Peptide:

  UBE2N/Ubc13 Peptide (NBP1-76593PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Ion exchange chromatography

Alternate Names for UBE2N/Ubc13 Antibody - BSA Free

• bendless-like ubiquitin conjugating enzyme
• Bendless-like ubiquitin-conjugating enzyme
• BLU
• EC 6.3.2.19
• MGC131857
• MGC8489
• UBC13
• UbcH13
• UbcH-ben
• UBE2N
• Ubiquitin carrier protein N
• ubiquitin-conjugating enzyme E2 N
• ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13)
• ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast)
• Ubiquitin-protein ligase N
• yeast UBC13 homolog

Background

Ubiquitin-conjugating enzyme 13 (Ubc13) was initially discovered in S. cerevisiae as a DNA-damage inducible protein involved in the error-free DNA postreplication repair pathway. It has recently been shown to be an important component of the Toll-like receptor and IL-1R signaling pathway. Signals from these pathways are relayed by a number of downstream molecules such as MyD88 and tumor necrosis factor receptor associated factor (TRAF6), ultimately activating various kinases and transcription factors. Ubc13 is part of a dimeric ubiquitin-conjugating enzyme complex also containing UEV1A (ubiquitin-conjugating enzyme E2 variant 1) that together with TRAF6 activates TAK1, a member of the mitogen-activated protein kinase kinase kinase family. The Ubc13-UEV1A complex also mediates the Lys-63 ubiquitination of TRAF-6, and this ubiquitination is essential for TAK1 activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UBE2N/Ubc13 Antibody (NBP1-76593)(1)

Publication using NBP1-76593

• Masuda Y, Suzuki M, Kawai H et al. En bloc transfer of polyubiquitin chains to PCNA in vitro is mediated by two different human E2-E3 pairs. Nucleic Acids Res. 2012-11-01 [PMID: 22904075]

Bioinformatics

• Gene Symbol: UBE2N
• Uniprot:
  • AAP35519(Human)