TYRP1 Antibody (CL4906) - BSA Free

Western Blot: TYRP1 Antibody (CL4906) [NBP2-61142] - Western blot analysis in human cell line SK-MEL-30.

Immunocytochemistry/Immunofluorescence: TYRP1 Antibody (CL4906) [NBP2-61142] - Staining of SK-MEL-30 cells using the Anti-TYRP1 monoclonal antibody, showing specific staining in the vesicles in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry: TYRP1 Antibody (CL4906) [NBP2-61142] - Staining of human tonsil shows no positivity in lymphoid cells as expected (negative control).

Immunohistochemistry: TYRP1 Antibody (CL4906) [NBP2-61142] - Staining of human skin shows strong cytoplasmic immunoreactivity in melanocytes.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: CL4906
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

TYRP1 Antibody (CL4906) - BSA Free Summary

• Description: Novus Biologicals Mouse TYRP1 Antibody (CL4906) - BSA Free (NBP2-61142) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from P17643, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (91%), Rat (91%). Backed by our 100% Guarantee.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TYRP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
• Control Peptide:

  TYRP1 Recombinant Protein Antigen (NBP2-61142PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for TYRP1 Antibody (CL4906) - BSA Free

• b-PROTEIN
• CAS25,6-dihydroxyindole-2-carboxylic acid oxidase
• Catalase B
• CATB
• EC 1.14.18
• EC 1.14.18.-
• EC 1.14.18.1
• Glycoprotein 75
• GP75DHICA oxidase
• Melanoma antigen gp75
• TRP-1
• TRPTYRP
• tyrosinase-related protein 1TRP1
• TYRRP

Background

TYRP1 encodes a melanosomal enzyme that belongs to the tyrosinase family and plays an important role in the melanin biosynthetic pathway. Defects in this gene are the cause of rufous oculocutaneous albinism and oculocutaneous albinism type III. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TYRP1