Tyrosine Hydroxylase Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-110] - Analysis of gelatin section of mouse brain (substantia nigra pars compacta) using Tyrosine Hydroxylase antibody. Image from verified ...read more
Immunocytochemistry/ Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-110] - Rabbit retina showing specific labeling of tyrosine hydroxylase in green.
Western Blot: Tyrosine Hydroxylase Antibody [NB300-110] - Analysis of rat caudate lysate showing specific immunolabeling of the ~60k tyrosine hydroxylase protein. 10 ug

Product Details

Summary
Reactivity Hu, Mu, Rt, Av, Ma, Rb, ReSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
0.15 mg/ml

Order Details

Tyrosine Hydroxylase Antibody Summary

Immunogen
Native rat tyrosine hydroxylase, purified from pheochromocytoma.
Localization
NB300-110 can be used as a marker for dopaminergic and noradrenergic neurons.
Marker
Neuronal Marker
Specificity
Specific for the ~60k tyrosine hydroxylase protein.
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
TH
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:1000
  • Immunohistochemistry-Paraffin
  • Immunohistochemistry Free-Floating
Application Notes
Immunocytochemistry use reported in literature (PMID: 21694758). Immunohistochemistry-Paraffin use reported in literature (PMID: 23690557). Use in Immunohistochemistry free-floating reported in scientific literature (PMID 26553597).
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Brain Postcentral Gyrus Lysate (NB820-59196)
Eye Lysate (NB820-59279)
Reviewed Applications
Read 3 Reviews rated 5
using
NB300-110 in the following applications:

Publications
Read Publications using
NB300-110 in the following applications:

Reactivity Notes

The antibody recognizes all mammalian and at least some nonmammalian forms of the enzyme in Western blots and in IHC/IF.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Concentration
0.15 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Tyrosine Hydroxylase Antibody

  • DYT14
  • DYT5b
  • EC 1.14.16
  • EC 1.14.16.2
  • TH
  • TYH dystonia 14
  • TYH
  • Tyrosine 3-hydroxylase
  • tyrosine 3-monooxygenase
  • Tyrosine Hydroxylase

Background

Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine, epinephrine and norepinephrine. Therefore the regulation of the TH enzyme represents the central means for controlling the synthesis of these important catecholamines. TH has a large molecular diversity, resulting from differential splicing of its mRNA, which is tissue specific and might result in long term changes in activity of the enzyme and the availability of neurotransmitter at various synapses. The presence of different DNA sequences at the TH locus confers susceptibility to various disorders of the brain including manic-depression and schizophrenia. Parkinson's disease is also considered a TH deficiency, as low dopamine levels are a consistent neurochemical abnormality.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Tyrosine Hydroxylase Antibody (NB300-110)(50)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Amphibian, Avian, Reptile.

We have publications tested in 6 applications: ICC/IF, IHC, IHC-Fr, IHC-FrFl, IHC-P, WB.


Filter By Application
ICC/IF
(20)
IHC
(7)
IHC-Fr
(9)
IHC-FrFl
(1)
IHC-P
(3)
WB
(2)
All Applications
Filter By Species
Human
(3)
Mouse
(20)
Rat
(6)
Amphibian
(1)
Avian
(6)
Reptile
(1)
All Species
Showing Publications 1 - 10 of 50. Show All 50 Publications.
Publications using NB300-110 Applications Species
Kim H, Kang H, Lee Y et al. Identification of transketolase as a target of PARIS in substantia nigra Biochem. Biophys. Res. Commun. 2017 Sep 20 [PMID: 28939041] (Human) Human
Dulcis D, Lippi G, Stark CJ, Do LH et al. Neurotransmitter Switching Regulated by miRNAs Controls Changes in Social Preference Neuron 2017 Sep 13 [PMID: 28867550] (Amphibian) Amphibian
Chen Y, Clark O, Woolley SC. Courtship song preferences in female zebra finches are shaped by developmental auditory experience. Proc. Biol. Sci. May 31 2017 [PMID: 28539523]
Skrzypecki J, Gawlak M, Huc T et al. Renal denervation decreases blood pressure and renal tyrosine hydroxylase but does not augment the effect of hypotensive drugs. Clin. Exp. Hypertens. Apr 27 2017 [PMID: 28448180] (Rat) Rat
Rivetti di Val Cervo P, Romanov RA, Spigolon G et al. Induction of functional dopamine neurons from human astrocytes in vitro and mouse astrocytes in a Parkinson's disease model. Nat. Biotechnol. Apr 10 2017 [PMID: 28398344] (ICC/IF, IHC, Human) ICC/IF, IHC Human
Jalewa J, Sharma MK, Gengler S, Holscher C. A novel GLP-1/GIP dual receptor agonist protects from 6-OHDA lesion in a rat model of Parkinson's disease. Neuropharmacology. 2017 Feb 20 [PMID: 28223210]
Villaescusa JC, Li B, Toledo EM et al. A PBX1 transcriptional network controls dopaminergic neuron development and is impaired in Parkinson's disease EMBO J. 2016 Jun 28 [PMID: 27354364]
van der Keylen P, Garreis F, Steigleder R et al. Involvement of catecholaminergic neurons in motor innervation of striated muscle in the mouse esophagus. Histochem Cell Biol. 2016 Jan 21 [PMID: 26794326]
Schubloom HE, Woolley SC. Variation in social relationships relates to song preferences and EGR1 expression in a female songbird. Dev Neurobiol. 2015 Dec 29 [PMID: 26713856]
Buckinx R, Van Remoortel S, Gijsbers R et al. Proof-of-concept: neonatal intravenous injection of adeno-associated virus vectors results in successful transduction of myenteric and submucosal neurons in the mouse small and large intestine. Neurogastroenterol. Motil. 2015 Nov 12 [PMID: 26564813] (IHC-Fr, Mouse) IHC-Fr Mouse
Show All 50 Publications.

Reviews for Tyrosine Hydroxylase Antibody (NB300-110) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Mouse.
We have 3 reviews tested in 1 application: IF.

Reviews using NB300-110:
Filter by Applications
IF
(2)
All Applications
Filter by Species
Mouse
(3)
All Species
Images Ratings Applications Species Date Details
 Tyrosine Hydroxylase NB300-110
Enlarge
reviewed by:
Bianca Kerschbaumer
Mouse 05/19/2017
View

Summary

Sample TestedBrain (substantia nigra)
SpeciesMouse
Lotajo0516o
 
reviewed by:
Anonymous
IF Mouse 08/05/2016
View

Summary

ApplicationImmunofluorescence
Sample Testedembryonic mouse stomach
SpeciesMouse

Blocking

Blocking Details2% normal donkey serum in 1x PBS with 0.1% Triton X-100, 1h at RT

Primary Anitbody

Dilution Ratiodilution 1:300 in blocking solution o/n 4°C

Secondary Antibody

Secondary DescriptionAlexa-fluor donkey anti-sheep IgG 488
Secondary Concentration1:400

Details

Detection NotesZeiss LSM700 microscope
Fixation Detailsfixation: 4% PFA 2h at 4°C, 30% sucrose o/n 4°C, frozen in OCT medium
Wash Description1x PBS, 3 times 10min
Immunofluorescence Tyrosine Hydroxylase NB300-110
Enlarge
reviewed by:
Anonymous
IF Mouse 10/23/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedGelatin Section of Mouse Brain (substantia nigra pars compacta)
SpeciesMouse
LotAJ0214B

Blocking

Blocking Details1h 4° C in 10% NHS + 0.5% Triton + TBS

Primary Anitbody

Dilution RatioOVER NIGTH 4°C in 2%NHS + 0.4% Triton + TBS + Ab (1:4000)

Secondary Antibody

Secondary DescriptionDonkey ant-sheep
Secondary Manufacturer Cat#Code: 713-165-147
Secondary Concentrationat RT in 2%NHS + 0.4% Triton + TBS + Ab (1:500)

Details

Detection Notes40X objective of confocal microscope
Wash DescriptionUsing gelatin sections we need two washing buffer: 0.1M PB and TBS

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Tyrosine Hydroxylase Antibody (NB300-110). (Showing 1 - 1 of 1 FAQs).

  1. On the NOVUS website, it showed that NB300-110 could be used to detect Tyrosine Hydroxylase in mammal by IHC-P experiment. Our customer would like to know whether it can be used on dog or not. We are looking forward to your nice help.
    • The immunogen which was used to generate our Tyrosine Hydroxylase antibody with catalogue number NB300-110 was native rat Tyrosine Hydroxylase that was purified from pheochromocytoma. The antibody recognizes all mammalian forms of the enzyme in Western blots and in IHC/IF, however we do not have any data from dog that I can share with you at the present time.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Tyrosine Hydroxylase Antibody (NB300-110)

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Blogs on Tyrosine Hydroxylase.

The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

Tyrosine hydroxylase - a marker for dopaminergic neurons in the central nervous system
Tyrosine hydroxylase is a member of the aromatic amino acid hydroxylase (AAAH) family.  It is expressed throughout the central nervous system (CNS) and catalyzes the conversion of tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), which can be, thr...  Read full blog post.

Tyrosine Hydroxylase - rate-limiting enzyme in catecholamine synthesis
Catecholamines are tyrosine-derived hormones that are produced in the adrenal gland. They include epinephrine, norepinephrine, and dopamine and are used as neurotransmitters by the central and peripheral nervous system. The rate limiting enzyme in...  Read full blog post.

A Big Guy for the Catecholamine Synthesis - Tyrosine hydroxylase (TH)
In the synthesis pathway for the catecholamines - dopamine, epinephrine, and norepinephrine, tyrosine hydroxylase is the rate-limiting enzyme. Through alternative mRNA splicing, a wide molecular diversity of TH isoforms are generated that are tissue-s...  Read full blog post.

Tyrosine Hydroxylase Deficiencies and Neurodegeneration
Tyrosine hydroxylase is the rate-limiting enzyme in the synthesis pathway of the catecholamines dopamine, epinephrine, and norepinephrine. Alternative mRNA splicing generates a wide molecular diversity of TH isoforms that are tissue specific and produ...  Read full blog post.

Tyrosine Hydroxylase Deficiency and Brain Disorders
 Tyrosine hydroxylase catalyzes the rate-limiting step in the biosynthesis of the catecholamines dopamine, norepinephrine, and epinephrine. A hallmark of Parkinson's disease is the loss of dopaminergic neurons in the substantia nigra. Mutations in cas...  Read full blog post.

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Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

Bianca Kerschbaumer
05/19/2017
Application:
Species: Mouse

 
Anonymous
08/05/2016
Application: IF
Species: Mouse

Anonymous
10/23/2014
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol TH
Entrez
Uniprot