Tyrosine Hydroxylase Antibody

Western Blot: Tyrosine Hydroxylase Antibody [NB300-110] - Analysis of rat caudate lysate showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase protein.

Western Blot: Tyrosine Hydroxylase Antibody [NB300-110] - Mice were injected with PBS (control group) or the dopaminergic neurotoxin MPTP. There is low concentration of TH in the striatum of mouse injected with MPTP. 30 ug total protein per lane. Antibody at 1:3000. Western blot image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-110] - Alpha-Synuclein overexpression or intracerebral seeding impacts the dopaminergic system. Increased levels of ser129-phosphosynuclein in the substantia nigra (arrows) indicate alpha-syn stress in PFF injected animals. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30050065/) licensed under a CC-BY licence.

Immunohistochemistry: Tyrosine Hydroxylase Antibody [NB300-110] - Mouse cryo-sections. Antibody at 1:500. Secondary antibody: dk-anti-sp Alexa Fluor488 at 1:500; 1 h. IHC image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-110] - Nmnat by itself does not protect dopaminergic neurons from MPP+ toxicity. Similar transduction efficiencies of the different lentiviruses were confirmed by quantifying the number of TH+ and GFP+ cells following transduction of dopaminergic cultures. Quantification of TH+ cell bodies and (E) TH+ neurites show that only WldS-transduced cultures protected neurites against MPP+. Data are normalized to control cultures and denote the mean +/- SEM of representative determinations made in three separate cultures. *p < 0.001. Image collected and cropped by CiteAb from the following publication (//molecularneurodegeneration.biomedcentral.com/articles/10.1186/1750-1326-7-5), licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: Tyrosine Hydroxylase Antibody [NB300-110] - Sagittal section of Pleurodeles waltl (amphibian) brain. The cell bodies of dopaminergic neurons in ventral tegmental area and dopaminergic fibers in striatum are stained with the TH antibody. IHC image submitted by a verified customer review.

Immunohistochemistry: Tyrosine Hydroxylase Antibody [NB300-110] - Immunohistochemistry analysis of gelatin section of mouse brain (substantia nigra pars compacta) using Tyrosine Hydroxylase antibody. IHC image submitted by a verified customer review.

• Reactivity: Hu, Mu, Rt, Am, Av, Ma, Rb, Re
• Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl, Single-Cell Western
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: 0.15 mg/ml

Tyrosine Hydroxylase Antibody Summary

• Immunogen: SDS-denatured, native rat tyrosine hydroxylase purified from pheochromocytoma.
• Localization: NB300-110 can be used as a marker for dopaminergic and noradrenergic neurons.
• Marker: Neuronal Marker
• Specificity: Specific for the ~60 kDa tyrosine hydroxylase protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: TH
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:1000
  • Immunohistochemistry-Paraffin
  • Single Cell Western
  • Western Blot 1:1000
• Application Notes: Immunocytochemistry use reported in literature (PMID 21694758). Immunohistochemistry-Paraffin use reported in literature (PMID 23690557) and customer review. Use in Immunohistochemistry free-floating reported in scientific literature (PMID 26553597).
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Brain Postcentral Gyrus Whole Tissue Lysate (Adult Whole Normal)
  Human Eye Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

The antibody recognizes all mammalian and at least some non-mammalian forms of the enzyme in Western blot and in IHC/IF. Amphibian reactivity reported in scientific literature (PMID: 28867550).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol
• Preservative: No Preservative
• Concentration: 0.15 mg/ml
• Purity: Antigen Affinity-purified

Alternate Names for Tyrosine Hydroxylase Antibody

• DYT14
• DYT5b
• EC 1.14.16
• EC 1.14.16.2
• TH
• TYH dystonia 14
• TYH
• Tyrosine 3-hydroxylase
• tyrosine 3-monooxygenase
• Tyrosine Hydroxylase

Background

The tetrameric enzyme tyrosine hydroxylase (TH), also designated as tyrosine 3-monooxygenase (TY3H), is the rate-limiting enzyme for catecholamine biosynthesis and has a theoretical molecular weight of 60 kDa. Encoded by the TH gene, TH catalyzes the conversion of the amino acid L-tyrosine to L-3,4- dihydroxyphenylalanine (L-dopa) in the central nervous system and adrenal medulla. The precursor of catecholamines, L-dopa, is converted to dopamine for use in the biosynthesis of neurotransmitters norepinephrine (noradrenaline) and epinephrine (adrenaline) (1) . Dysfunction in the TH gene, and thus the enzymatic activity by TH, dysregulates catecholamine synthesis.

Two transcription factor binding sites in the proximal region of the TH gene, the TPA-responsive element (TRE) and the c-AMP responsive element (CRE), have been implicated in the complex regulation of the TH gene. Dysregulation of breakdown for the amino acid, tyrosine, by TH is a result of a genetic disorder that results in Tyrosinemia (high levels of tyrosine in the blood, tissue and organs).

Tyrosine hydroxylase deficiency is a disorder that primarily affects movement, where individuals display symptoms that include lack of coordination when walking, postural tremors and unusual body positioning. TH deficient dopamine-responsive dystonia (DRD), also known as Segawa syndrome, is a rare genetic disorder that is associated with low levels of TH and is diagnosed during childhood with characteristic symptoms including increased muscle tone (dystonia) and signs of Parkinsonism like bradykinesia, tremors, rigidity and postural instability (2). Correspondingly, TH is also linked to Parkinson's disease in older adults, where low dopamine levels are a consistent neurochemical abnormality. Functional polymorphisms of the TH gene may be involved in the pathogenesis of neuropsychiatric diseases such as schizophrenia and other affective disorders where dopamine is often dysregulated (3).

References

1. Hamanaka, Y., & Mizunami, M. (2019). Tyrosine hydroxylase-immunoreactive neurons in the mushroom body of the field cricket, Gryllus bimaculatus. Cell Tissue Res, 376(1), 97-111. doi:10.1007/s00441-018-2969-9

2. Li, L., & Zhou, F. M. (2013). Parallel dopamine D1 receptor activity dependence of l-Dopa-induced normal movement and dyskinesia in mice. Neuroscience, 236, 66-76. doi:10.1016/j.neuroscience.2012.12.065

3. Borkar, C. D., Bharne, A. P., Nagalakshmi, B., Sakharkar, A. J., Subhedar, N. K., & Kokare, D. M. (2018). Cocaine- and Amphetamine-Regulated Transcript Peptide (CART) Alleviates MK-801-Induced Schizophrenic Dementia-Like Symptoms. Neuroscience, 375, 94-107. doi:10.1016/j.neuroscience.2018.01.056

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tyrosine Hydroxylase Antibody (NB300-110)(84)

Publications using NB300-110

• Brandon A, Cui X, Luan W et al. Prenatal hypoxia alters the early ontogeny of dopamine neurons Translational psychiatry 2022-06-07 [PMID: 35672280] (IHC, Mouse)
• Pertile R, Kiltschewskij D, Geaghan M et al. Developmental Vitamin D-Deficiency Increases the Expression of MicroRNAs Involved in Dopamine Neuron Development SSRN Electronic Journal 2022-02-09 [PMID: 35642827]
• Kameneva P, Melnikova VI, Kastriti ME et al. Serotonin limits generation of chromaffin cells during adrenal organ development Nature communications 2022-05-25 [PMID: 35614045] (IHC-Fr, Mouse, Rat)
• Arotcarena ML, Soria FN, Cunha A et al. Acidic nanoparticles protect against alpha-synuclein-induced neurodegeneration through the restoration of lysosomal function Aging cell 2022-03-23 [PMID: 35318803] (IHC, Mouse)
• Kabelik D, Julien AR, Waddell BR et al. Aggressive but not reproductive boldness in male green anole lizards correlates with baseline vasopressin activity Hormones and behavior 2022-01-20 [PMID: 35066329] (IHC, Reptile)
• Barr HJ, Wall EM, Woolley SC Dopamine in the songbird auditory cortex shapes auditory preference Current biology : CB 2021-08-20 [PMID: 34450091]
• Xiang C, Chen P, Zhang Q et al. Intestinal microbiota modulates adrenomedullary response through Nod1 sensing in chromaffin cells iScience 2021-08-01 [PMID: 34381974] (IHC-P)
• Kameneva P, Artemov AV, Kastriti ME et al. Single-cell transcriptomics of human embryos identifies multiple sympathoblast lineages with potential implications for neuroblastoma origin Nature genetics 2021-04-08 [PMID: 33833454]
• Hong J, Adam RJ, Gao L et al. Macrophage activation in stellate ganglia contributes to lung injury-induced arrhythmogenesis in male rats Acta physiologica (Oxford, England) 2021-04-05 [PMID: 33817984]
• Evans RC, Twedell EL, Zhu M et al. Functional Dissection of Basal Ganglia Inhibitory Inputs onto Substantia Nigra Dopaminergic Neurons Cell Rep 2020-09-15 [PMID: 32937133] (ICC/IF, Mouse)
• Garcia-Dominguez I, Suarez-Pereira I, Santiago M et al. Selective deletion of Caspase-3 gene in the dopaminergic system exhibits autistic-like behaviour Prog. Neuropsychopharmacol. Biol. Psychiatry 2020-07-04 [PMID: 32634539] (IHC, Mouse)
• Romanov R, Tretiakov E, Kastriti M et al. Molecular design of hypothalamus development Nature 2020-05-06 [PMID: 32499648] (IHC, Human, Mouse)
• Shah S, Maric D, Denaro F et al. Nitrosative Stress Is Associated with Dopaminergic Dysfunction in the HIV-1 Transgenic Rat Am. J. Pathol. 2019-07-01 [PMID: 31230667]
• Kastriti ME, Kameneva P, Kamenev D et al. Schwann Cell Precursors Generate the Majority of Chromaffin Cells in Zuckerkandl Organ and Some Sympathetic Neurons in Paraganglia Front Mol Neurosci 2019-01-25 [PMID: 30740044] (ICC/IF, Mouse)
• Theofilopoulos S, Abreu de Oliveira WA, Yang S et al. 24(S),25-Epoxycholesterol and cholesterol 24S-hydroxylase (CYP46A1) overexpression promote midbrain dopaminergic neurogenesis in vivo J. Biol. Chem. 2019-01-17 [PMID: 30655290] (IHC, Mouse)
• Garcia-Dominguez I, Vesela K, Garcia-Revilla J et al. Peripheral Inflammation Enhances Microglia Response and Nigral Dopaminergic Cell Death in an in vivo MPTP Model of Parkinson's Disease. Front Cell Neurosci. 2018-11-06 [PMID: 30459561] (IHC, Mouse)
• Kabelik D, Weitekamp CA, Choudhury SC et al. Neural activity in the social decision-making network of the brown anole during reproductive and agonistic encounters Horm Behav. [PMID: 30342012] (IHC, Reptile)
• Yang Shanzheng, Toledo Enrique M, Rosmaninho Pedro et al. A Zeb2-miR-200c loop controls midbrain dopaminergic neuron neurogenesis and migration. Communications Biology 2018-06-25 [PMID: 30271956] (IHC-P, Mouse)
• Barr HJ, Woolley SC. Developmental auditory exposure shapes responses of catecholaminergic neurons to socially-modulated song Sci Rep 2018-08-06 [PMID: 30082796] (ICC/IF, Avian)
• Milanese C, Cerri S, UlusoY A et al. Activation of the DNA damage response in vivo in synucleinopathy models of Parkinson's disease. Cell Death Dis. 2018-07-26 [PMID: 30050065] (ICC/IF, Mouse)
• Okawa S, Salto C, Ravichandran S et al. Transcriptional synergy as an emergent property defining cell subpopulation identity enables population shift. Nat Commun. 2018-07-03 [PMID: 29968757] (ICC/IF, Human)
• Luan W, Hammond LA, Vuillermot S et al. Maternal Vitamin D Prevents Abnormal Dopaminergic Development and Function in a Mouse Model of Prenatal Immune Activation. Sci Rep 2018-06-27 [PMID: 29950608] (IHC, Mouse)
• Shaltouki A, Hsieh CH, Kim MJ, Wang X. Alpha-synuclein delays mitophagy and targeting Miro rescues neuron loss in Parkinson's models Acta Neuropathol. 2018-06-09 [PMID: 29923074] (Human)
• Dai JB, Chen Y, Sakata JT. EGR-1 expression in catecholamine-synthesizing neurons reflects auditory learning and correlates with responses in auditory processing areas. Neuroscience. 2018-03-27 [PMID: 29601855] (Avian)
• Van Ruijssevelt L, Chen Y et al. fMRI Reveals a Novel Region for Evaluating Acoustic Information for Mate Choice in a Female Songbird. Curr Biol 2018-05-03 [PMID: 29478859] (ICC/IF, Finch)
  
  Details:
  Taeniopygia guttata (Zebra finch)
• Abdellah N, Van Remoortel S, Mohey-Elsaeed O et al. Neuropeptide AF induces piecemeal degranulation in murine mucosal mast cells: A new mediator in neuro-immune communication in the intestinal lamina propria? Anat Rec (Hoboken) 2018-01-18 [PMID: 29346716] (Mouse)
• Friend L, Weed J, Sandoval P, Nufer T et al. CB1-Dependent Long-Term Depression in Ventral Tegmental Area GABA Neurons: A Novel Target for Marijuana J. Neurosci. 2017-10-16 [PMID: 29038246] (IHC, Mouse)
• Sinharay S, Lee D et al. Cross-sectional and longitudinal small animal PET shows pre and post-synaptic striatal dopaminergic deficits in an animal model of HIV. Nucl Med Biol 2017-01-12 [PMID: 29031113] (IHC, Rat)
• Kim H, Kang H, Lee Y et al. Identification of transketolase as a target of PARIS in substantia nigra Biochem. Biophys. Res. Commun. 2017-09-20 [PMID: 28939041] (Human)
• Dulcis D, Lippi G, Stark CJ, Do LH et al. Neurotransmitter Switching Regulated by miRNAs Controls Changes in Social Preference Neuron 2017-09-13 [PMID: 28867550] (Amphibian)
• Chen Y, Clark O, Woolley SC. Courtship song preferences in female zebra finches are shaped by developmental auditory experience. Proc. Biol. Sci. 2017-05-31 [PMID: 28539523]
• Skrzypecki J, Gawlak M, Huc T et al. Renal denervation decreases blood pressure and renal tyrosine hydroxylase but does not augment the effect of hypotensive drugs. Clin. Exp. Hypertens. 2017-04-27 [PMID: 28448180] (Rat)
• Rivetti di Val Cervo P, Romanov RA, Spigolon G et al. Induction of functional dopamine neurons from human astrocytes in vitro and mouse astrocytes in a Parkinson's disease model. Nat. Biotechnol. 2017-04-10 [PMID: 28398344] (ICC/IF, IHC, Human)
• Jalewa J, Sharma MK, Gengler S, Holscher C. A novel GLP-1/GIP dual receptor agonist protects from 6-OHDA lesion in a rat model of Parkinson's disease. Neuropharmacology. 2017-02-20 [PMID: 28223210]
• La Manno G, Gyllborg D et al. Molecular Diversity of Midbrain Development in Mouse, Human, and Stem Cells. Cell 2016-06-10 [PMID: 27716510] (ICC/IF, Human)
• Villaescusa JC, Li B, Toledo EM et al. A PBX1 transcriptional network controls dopaminergic neuron development and is impaired in Parkinson's disease EMBO J. 2016-06-28 [PMID: 27354364]
• van der Keylen P, Garreis F, Steigleder R et al. Involvement of catecholaminergic neurons in motor innervation of striated muscle in the mouse esophagus. Histochem. Cell Biol. 2016-01-21 [PMID: 26794326]
• Schubloom HE, Woolley SC. Variation in social relationships relates to song preferences and EGR1 expression in a female songbird. Dev Neurobiol. 2015-12-29 [PMID: 26713856]
• Buckinx R, Van Remoortel S, Gijsbers R et al. Proof-of-concept: neonatal intravenous injection of adeno-associated virus vectors results in successful transduction of myenteric and submucosal neurons in the mouse small and large intestine. Neurogastroenterol. Motil. 2015-11-12 [PMID: 26564813] (IHC-Fr, Mouse)
• Merrill CB, Friend LN, Newton ST et al. Ventral tegmental area dopamine and GABA neurons: Physiological properties and expression of mRNA for endocannabinoid biosynthetic elements. Sci Rep. 2015-11-10 [PMID: 26553597] (IHC-FrFl, Mouse)
• Hodson EJ, Nicholls LG, Turner PJ et al. Regulation of ventilatory sensitivity and carotid body proliferation in hypoxia by the PHD2/HIF-2 pathway. J. Physiol. (Lond.) 2015-09-04 [PMID: 26337139] (IHC-P, Mouse)
• Matheson LE, Sun H, Sakata JT. Forebrain circuits underlying the social modulation of vocal communication signals. Dev Neurobiol. 2015-05-11 [PMID: 25959605] (ICC/IF, Avian)
• Matheson LE, Sakata JT. Catecholaminergic contributions to vocal communication signals Eur. J. Neurosci. 2015-04-07 [PMID: 25847067] (IHC-Fr, Avian)
  
  Details:
  Tyrosine Hydroxylase antibody was used for IHC-FrFl application on paraformaldehyde - sucrose fixed coronal 40um brain sections / free floating sections from adult male Bengalese finches. See full text for the detailed protocol and the data is shown in Figure 2 & 3.
• Mansley MK, Neuhuber W, Korbmacher C, Bertog M. Norepinephrine stimulates the epithelial sodium channel (ENaC) in cortical collecting duct cells via alpha2-adrenoceptors. Am. J. Physiol. Renal Physiol. 2014-12-17 [PMID: 25520009] (IHC-Fr, Mouse)
• Kelly AM, Goodson JL. Functional interactions of dopamine cell groups reflect personality, sex, and social context in highly social finches. Behav. Brain Res. 2015-03-01 [PMID: 25496780]
• Dmitry Usoskin, Alessandro Furlan, Saiful Islam et al. Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing. Nature Neuroscience 2014-11-24 [PMID: 25420068]
• Buckinx R, Bagyanszki M, Avula Lr et al. Expression of corticotropin-releasing factor and urocortins in the normal and Schistosoma mansoni-infected mouse ileum Cell Tissue Res. 2014-10-31 [PMID: 25358399] (IHC-Fr, Mouse)
  
  Details:
  Tyrosine Hydroxylase antibody used at 1:500 dilution for IHC-Fr on normal and Schistosoma mansoni-infected mouse ileum (data not shown in article)
• Tsika E, Kannan M, Foo CS et al. Conditional expression of Parkinson's disease-related R1441C LRRK2 in midbrain dopaminergic neurons of mice causes nuclear abnormalities without neurodegeneration. Neurobiol Dis 2014-11-01 [PMID: 25174890] (WB)
• Knaryan VH, Samantaray S, Park S et al. SNJ-1945, a calpain inhibitor, protects SH-SY5Y cells against MPP+ and rotenone. J Neurochem 2013-11-29 [PMID: 24341912] (ICC/IF, Human)
• Schrodl F, Trost A, Strohmaier C et al. Rat choroidal pericytes as a target of the autonomic nervous system. Cell Tissue Res 2013-12-14 [PMID: 24337689] (ICC/IF, IHC-Fr, Rat)
• Bishop T, Talbot NP, Turner PJ et al. Carotid body hyperplasia and enhanced ventilatory responses to hypoxia in mice with heterozygous deficiency of PHD2. J Physiol 2013-05-20 [PMID: 23690557] (IHC-P, Mouse)
• Furlan A, Lubke M, Adameyko I et al. The transcription factor Hmx1 and growth factor receptor activities control sympathetic neurons diversification. EMBO J 2013-04-16 [PMID: 23591430] (ICC/IF, IHC, Mouse)
• Ditting T, Freisinger W, Siegel K et al. Tonic postganglionic sympathetic inhibition induced by afferent renal nerves? Hypertension 2012-02-01 [PMID: 22215714] (ICC/IF, Rat)
• Kostuk EW, Balbir A, Fujii K, Fujioka A, Pichard LE, Shirahata M. Divergent postnatal development of the carotid body in DBA/2J and A/J strains of mice. J Appl Physiol;112(3):490-500. 2012-02-01 [PMID: 22074716] (IHC-P, Mouse)
• Lai I, Romoli B, Keisler M et al. METHODS AND MATERIALS Animals bioRxiv 2021-01-01
• Bernstein AI, O'Malley KL. MPP+-induces PUMA- and p53-dependent, but ATF3-independent cell death Toxicol Lett 2013-03-14 [PMID: 23500530] (ICC/IF, Mouse)
• Kabelik D, Alix VC, Burford ER, Singh LJ. Aggression- and sex-induced neural activity across vasotocin populations in the brown anole Horm Behav 2012-11-30 [PMID: 23201179] (ICC/IF, IHC, Reptile)
• Hirasawa H, Betensky RA, Raviola E. Corelease of Dopamine and GABA by a Retinal Dopaminergic Neuron J Neurosci 2012-09-19 [PMID: 22993444] (ICC/IF, IHC-Fr, Mouse)
• Goodson JL, Wilson LC, Schrock SE. To flock or fight: Neurochemical signatures of divergent life histories in sparrows Proc Natl Acad Sci U S A 2012-06-26 [PMID: 22723363] (ICC/IF, IHC, Avian)
• Zhou B Novel skeletal stem and progenitor cells and their regulation Thesis 2022-01-01
• Hundahl CA, Fahrenkrug J, Luuk H et al. Restricted expression of Neuroglobin in the mouse retina and co-localization with Melanopsin and Tyrosine Hydroxylase Biochem Biophys Res Commun 2012-08-17 [PMID: 22820193] (ICC/IF, IHC, WB, Mouse)
• Chapman RJ, Lall VK, Maxeiner S, Willecke K, Deuchars J, King AE. Localization of neurones expressing the gap junction protein Connexin45 within the adult spinal dorsal horn: a study using Cx45-eGFP reporter mice. Brain Struct Funct. 2012-05-26 [PMID: 22638825] (IHC, Mouse)
• Antenor-Dorsey JA, O'Malley KL. WldS but not Nmnat1 protects dopaminergic neurites from MPP+ neurotoxicity Mol Neurodegener 2012-02-01 [PMID: 22315973] (ICC/IF, Mouse)
• Kingsbury MA, Kelly AM, Schrock SE, Goodson JL. Mammal-like organization of the avian midbrain central gray and a reappraisal of the intercollicular nucleus. PLoS One;6(6):e20720. 2011-01-01 [PMID: 21694758] (ICC/IF, Avian, Mouse)
• Halbig TD, Creighton J, Assuras S et al. Preserved emotional modulation of motor response time despite psychomotor slowing in young-old adults. Int J Neurosci. 2011-08-01 [PMID: 21574890]
• Contini M, Lin B, Kobayashi K et al. Synaptic input of ON-bipolar cells onto the dopaminergic neurons of the mouse retina. J Comp Neurol. 2010-01-01 [PMID: 20394057] (ICC/IF, Mouse)
• Ditting T, Tiegs G, Rodionova K et al. Do distinct populations of dorsal root ganglion neurons account for the sensory peptidergic innervation of the kidney? Am J Physiol Renal Physiol. 2009 Nov;297(5):F1427-34. [PMID: 19692481] (IHC, Rat)
• Goodson JL, Kabelik D, Kelly AM et al. Midbrain dopamine neurons reflect affiliation phenotypes in finches are tightly coupled to courtship. PNAS 2009;106(21):8737-8742. [PMID: 19439662] (ICC/IF, Avian)
• Hirasawa H, Puopolo M, Raviola E. Extrasynaptic Release of GABA by Retinal Dopaminergic Neurons. J Neurophysiol 2009;102(1):146-158. [PMID: 19403749] (ICC/IF, Mouse)
• Kwon OB, Paredes D, Gonzalez CM et al. Neuregulin-1 regulates LTP at CA1 hippocampal synapses through activation of dopamine D4 receptors. PNAS;105(40):15587-15592. 2008-01-01 [PMID: 18832154] (ICC/IF, Rat)
• Kestler C, Neuhuber WL, Raab M. Distribution of P2X(3) receptor immunoreactivity in myenteric ganglia of the mouse esophagus. Histochem Cell Biol. 2009-01-01 [PMID: 18810483] (IHC-Fr, Mouse)
• Veelken R, Vogel E-M, Hilgers K et al. Autonomic Renal Denervation Ameliorates Experimental Glomerulonephritis. J Am Soc Nephrol; 19(7):1371-1378. 2008-01-01 [PMID: 18400940] (ICC/IF, Rat)
• Kraus T, Neuhuber WL, Raab M. Distribution of vesicular glutamate transporter 1 (VGLUT1) in the mouse esophagus. Cell Tissue Res 2007-08-01 [PMID: 17508221]
• Puopolo, M, Raviola, E, Bean, B P. Roles of Subthreshold Calcium Current Sodium Current in Spontaneous Firing of Mouse Midbrain Dopamine Neurons. ; 27, 645-656. 2007-01-01 [PMID: 17234596] (ICC/IF, Mouse)
• Raab M, Neuhuber WL. Intraganglionic laminar endings and their relationships with neuronal and glial structures of myenteric ganglia in the esophagus of rat and mouse. Histochem Cell Biol. 2004-11-01 [PMID: 15378379] (IHC-Fr, Mouse)
• Goodson JL, Evans AK, Lindberg L. Chemoarchitectonic Subdivisions of the Songbird Septum and a Comparative Overview of Septum Chemical Anatomy in Jawed Vertebrates. J Comp Neurol 2004-05-31 [PMID: 15116393] (ICC/IF, Avian)
• Contini, M et al. GABAergic synapses made by a retinal dopaminergic neuron. PNAS; 100(3):1358-1363. 2003-01-01 [PMID: 12547914] (IHC-Fr, Mouse)
• Cortese JF, Caraballo A, Contreras CE, Plowe CV. Origin and dissemination of Plasmodium falciparum drug-resistance mutations in South America. J Infect Dis 2002-10-01 [PMID: 12232841]
• Guo, Z et al. Central pathways in the pons midbrain involved in cardiac sympathoexcitatory reflexes in cats. Neuroscience; 113(2):435-447. 2002-01-01 [PMID: 12127100]
• Guo ZL, Lai HC, Longhurst JC. Medullary pathways involved in cardiac sympathoexcitatory reflexes in the cat. Brain Res;925(1):55-66. 2002-01-18 [PMID: 11755900]
• Nocjar, C et al. Localization of 5-HT2A receptors on dopamine cells in subnuclei of the midbrain A10 cell group. Neuroscience; 113(1): 163-176. 2002-01-01 [PMID: 11955720]

Reviews for Tyrosine Hydroxylase Antibody (NB300-110) (5)

Average Rating: 5

(Based on 5 reviews)

We have 5 reviews tested in 2 species: Mouse, Pleurodeles waltl.

reviewed by: Verified Customer IHC-P Pleurodeles waltl 07/03/2019

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: brain section
• Species: Pleurodeles waltl
• Lot: ajo1217p

reviewed by: Verified Customer WB Mouse 10/17/2018

Summary

• Application: Western Blot
• Sample Tested: Brain (corpus striatum)
• Species: Mouse
• Lot: ajo109

Comments

• Comments: It works very well at 1:3,000 dilution when loading gels with 30 ug of protein extracts.

reviewed by: Verified Customer ICC Mouse 05/19/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Brain (substantia nigra)
• Species: Mouse
• Lot: ajo0516o

reviewed by: Viktoria Knoflach IF Mouse 08/05/2016

Summary

• Application: Immunofluorescence
• Sample Tested: embryonic mouse stomach
• Species: Mouse

reviewed by: Luana Barone IF Mouse 10/23/2014

Summary

• Application: Immunofluorescence
• Sample Tested: Gelatin Section of Mouse Brain (substantia nigra pars compacta)
• Species: Mouse
• Lot: AJ0214B

FAQs for Tyrosine Hydroxylase Antibody (NB300-110). (Showing 1 - 1 of 1 FAQs).

1. On the NOVUS website, it showed that NB300-110 could be used to detect Tyrosine Hydroxylase in mammal by IHC-P experiment. Our customer would like to know whether it can be used on dog or not. We are looking forward to your nice help.
   • The immunogen which was used to generate our Tyrosine Hydroxylase antibody with catalogue number NB300-110 was native rat Tyrosine Hydroxylase that was purified from pheochromocytoma. The antibody recognizes all mammalian forms of the enzyme in Western blots and in IHC/IF, however we do not have any data from dog that I can share with you at the present time.

Bioinformatics

• Gene Symbol: TH
• Entrez:
  • 7054(Human)
  • 21823(Mouse)
  • 25085(Rat)
• Uniprot:
  • P04177(Rat)