Tyrosine Hydroxylase Antibody


Western Blot: Tyrosine Hydroxylase Antibody [NB300-110] - Analysis of rat caudate lysate showing specific immunolabeling of the ~60k tyrosine hydroxylase protein. 10 ug
Immunocytochemistry/ Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-110] - Rabbit retina showing specific labeling of tyrosine hydroxylase in green.
Immunocytochemistry/ Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-110] - Analysis of gelatin section of mouse brain (substantia nigra pars compacta) using Tyrosine Hydroxylase antibody. Image from verified ...read more

Product Details

Reactivity Hu, Mu, Rt, Av, Ma, Rb, ReSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl
0.15 mg/ml

Order Details

Tyrosine Hydroxylase Antibody Summary

Native rat tyrosine hydroxylase, purified from pheochromocytoma.
NB300-110 can be used as a marker for dopaminergic and noradrenergic neurons.
Neuronal Marker
Specific for the ~60k tyrosine hydroxylase protein.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
10mM HEPES [pH7.5], 150 mM NaCl, 100ug/ml BSA, and 50% glycerol
No Preservative
0.15 mg/ml
Immunogen affinity purified


  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Frozen 1:1000
  • Immunohistochemistry-Paraffin
  • Immunohistochemistry Free-Floating
Application Notes
Immunocytochemistry use reported in literature (PMID: 21694758). Immunohistochemistry-Paraffin use reported in literature (PMID: 23690557). Use in Immunohistochemistry free-floating reported in scientific literature (PMID 26553597). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
60 kDa
Reviewed Applications
Read 2 Reviews rated 5
NB300-110 in the following applications:

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NB300-110 in the following applications:

Reactivity Notes

The antibody recognizes all mammalian and at least some nonmammalian forms of the enzyme in Western blots and in IHC/IF.

Alternate Names for Tyrosine Hydroxylase Antibody

  • DYT14
  • DYT5b
  • EC 1.14.16
  • EC
  • TH
  • TYH dystonia 14
  • TYH
  • Tyrosine 3-hydroxylase
  • tyrosine 3-monooxygenase
  • tyrosine hydroxylase


Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine, epinephrine and norepinephrine. Therefore the regulation of the TH enzyme represents the central means for controlling the synthesis of these important catecholamines. TH has a large molecular diversity, resulting from differential splicing of its mRNA, which is tissue specific and might result in long term changes in activity of the enzyme and the availability of neurotransmitter at various synapses. The presence of different DNA sequences at the TH locus confers susceptibility to various disorders of the brain including manic-depression and schizophrenia. Parkinson's disease is also considered a TH deficiency, as low dopamine levels are a consistent neurochemical abnormality.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt
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Species: Hu, Mu, Rt, Ca, Mk
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Av, Ch, GP, Pm
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-WhMt
Species: Hu, Rt, Po, Bv
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt, Po, Ca, Ch, ChHa, Fe, Op, Other, Pm
Applications: WB, Simple Western
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Species: Hu, Po
Applications: WB, ELISA, Flow, IHC, IHC-Fr, IHC-P, In vitro, RIA
Species: Mu, Rt, Hu(-)
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, GP, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu
Applications: WB, IHC, IHC-Fr, IHC-P, PEP-ELISA
Species: Mu, Rt
Applications: WB, IHC, Neut
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC
Species: Hu, Mu, Rt, Fe, GP, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, GS

Publications for Tyrosine Hydroxylase Antibody (NB300-110)(43)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Avian, Reptile.

We have publications tested in 6 applications: ICC/IF, IHC, IHC-Fr, IHC-FrFl, IHC-P, WB.

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Showing Publications 1 - 10 of 43. Show All 43 Publications.
Publications using NB300-110 Applications Species
Villaescusa JC, Li B, Toledo EM et al. A PBX1 transcriptional network controls dopaminergic neuron development and is impaired in Parkinson's disease EMBO J. 2016 Jun 28 [PMID:27354364]
van der Keylen P, Garreis F, Steigleder R et al. Involvement of catecholaminergic neurons in motor innervation of striated muscle in the mouse esophagus. Histochem Cell Biol. 2016 Jan 21 [PMID:26794326]
Schubloom HE, Woolley SC. Variation in social relationships relates to song preferences and EGR1 expression in a female songbird. Dev Neurobiol. 2015 Dec 29 [PMID:26713856]
Buckinx R, Van Remoortel S, Gijsbers R et al. Proof-of-concept: neonatal intravenous injection of adeno-associated virus vectors results in successful transduction of myenteric and submucosal neurons in the mouse small and large intestine. Neurogastroenterol. Motil. 2015 Nov 12 [PMID:26564813] (IHC-Fr, Mouse) IHC-Fr Mouse
Merrill CB, Friend LN, Newton ST et al. Ventral tegmental area dopamine and GABA neurons: Physiological properties and expression of mRNA for endocannabinoid biosynthetic elements. Sci Rep. 2015 Nov 10 [PMID:26553597] (IHC-FrFl, Mouse) IHC-FrFl Mouse
Hodson EJ, Nicholls LG, Turner PJ et al. Regulation of ventilatory sensitivity and carotid body proliferation in hypoxia by the PHD2/HIF-2 pathway. J. Physiol. (Lond.) 2015 Sep 04 [PMID:26337139] (IHC-P, Mouse) IHC-P Mouse
Matheson LE, Sun H, Sakata JT. Forebrain circuits underlying the social modulation of vocal communication signals. Dev Neurobiol. 2015 May 11 [PMID:25959605] (ICC/IF, Avian) ICC/IF Avian
Matheson LE, Sakata JT. Catecholaminergic contributions to vocal communication signals Eur. J. Neurosci. 2015 Apr 07 [PMID:25847067] (IHC-Fr, Avian) IHC-Fr Avian
Mansley MK, Neuhuber W, Korbmacher C, Bertog M. Norepinephrine stimulates the epithelial sodium channel (ENaC) in cortical collecting duct cells via alpha2-adrenoceptors. Am. J. Physiol. Renal Physiol. 2014 Dec 17 [PMID:25520009] (IHC-Fr, Mouse) IHC-Fr Mouse
Kelly AM, Goodson JL. Functional interactions of dopamine cell groups reflect personality, sex, and social context in highly social finches. Behav. Brain Res. 2015 Mar 01 [PMID:25496780]
Show All 43 Publications.

Reviews for Tyrosine Hydroxylase Antibody (NB300-110) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.
We have 2 review tested in 1 application: IF.

Reviews using NB300-110:
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Images Ratings Applications Species Date Details
reviewed by:
Mouse 08/05/2016


Sample Testedembryonic mouse stomach


Blocking Details2% normal donkey serum in 1x PBS with 0.1% Triton X-100, 1h at RT

Primary Anitbody

Dilution Ratiodilution 1:300 in blocking solution o/n 4°C

Secondary Antibody

Secondary DescriptionAlexa-fluor donkey anti-sheep IgG 488
Secondary Concentration1:400


Detection NotesZeiss LSM700 microscope
Fixation Detailsfixation: 4% PFA 2h at 4°C, 30% sucrose o/n 4°C, frozen in OCT medium
Wash Description1x PBS, 3 times 10min
Immunofluorescence Tyrosine Hydroxylase NB300-110
reviewed by:
IF Mouse 10/23/2014


Sample TestedGelatin Section of Mouse Brain (substantia nigra pars compacta)


Blocking Details1h 4° C in 10% NHS + 0.5% Triton + TBS

Primary Anitbody

Dilution RatioOVER NIGTH 4°C in 2%NHS + 0.4% Triton + TBS + Ab (1:4000)

Secondary Antibody

Secondary DescriptionDonkey ant-sheep
Secondary Manufacturer Cat#Code: 713-165-147
Secondary Concentrationat RT in 2%NHS + 0.4% Triton + TBS + Ab (1:500)


Detection Notes40X objective of confocal microscope
Wash DescriptionUsing gelatin sections we need two washing buffer: 0.1M PB and TBS

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Tyrosine Hydroxylase Antibody (NB300-110). (Showing 1 - 1 of 1 FAQs).

  1. On the NOVUS website, it showed that NB300-110 could be used to detect Tyrosine Hydroxylase in mammal by IHC-P experiment. Our customer would like to know whether it can be used on dog or not. We are looking forward to your nice help.
    • The immunogen which was used to generate our Tyrosine Hydroxylase antibody with catalogue number NB300-110 was native rat Tyrosine Hydroxylase that was purified from pheochromocytoma. The antibody recognizes all mammalian forms of the enzyme in Western blots and in IHC/IF, however we do not have any data from dog that I can share with you at the present time.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Blogs on Tyrosine Hydroxylase.

The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

Tyrosine hydroxylase - a marker for dopaminergic neurons in the central nervous system
Tyrosine hydroxylase is a member of the aromatic amino acid hydroxylase (AAAH) family.  It is expressed throughout the central nervous system (CNS) and catalyzes the conversion of tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), which can be, thr...  Read full blog post.

Tyrosine Hydroxylase - rate-limiting enzyme in catecholamine synthesis
Catecholamines are tyrosine-derived hormones that are produced in the adrenal gland. They include epinephrine, norepinephrine, and dopamine and are used as neurotransmitters by the central and peripheral nervous system. The rate limiting enzyme in...  Read full blog post.

A Big Guy for the Catecholamine Synthesis - Tyrosine hydroxylase (TH)
In the synthesis pathway for the catecholamines - dopamine, epinephrine, and norepinephrine, tyrosine hydroxylase is the rate-limiting enzyme. Through alternative mRNA splicing, a wide molecular diversity of TH isoforms are generated that are tissue-s...  Read full blog post.

Tyrosine Hydroxylase Deficiencies and Neurodegeneration
Tyrosine hydroxylase is the rate-limiting enzyme in the synthesis pathway of the catecholamines dopamine, epinephrine, and norepinephrine. Alternative mRNA splicing generates a wide molecular diversity of TH isoforms that are tissue specific and produ...  Read full blog post.

Tyrosine Hydroxylase Deficiency and Brain Disorders
 Tyrosine hydroxylase catalyzes the rate-limiting step in the biosynthesis of the catecholamines dopamine, norepinephrine, and epinephrine. A hallmark of Parkinson's disease is the loss of dopaminergic neurons in the substantia nigra. Mutations in cas...  Read full blog post.

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Gene Symbol TH

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