Tyrosinase Antibody

Western Blot: Tyrosinase Antibody [NBP2-20770] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Tyrosinase antibody [N3C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Product Discontinued

Tyrosinase Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Tyrosinase. The exact sequence is proprietary.
• Localization: Melanosome membrane; Single-pass type I membrane protein
• Predicted Species: Mouse (90%), Rat (92%), Porcine (94%), Bovine (92%), Rabbit (92%), Canine (90%), Sheep (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TYR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for Tyrosinase Antibody

• CMM8
• EC 1.14.18.1
• LB24-AB
• Monophenol monooxygenase
• OCA1A
• OCAIA
• SHEP3
• SK29-AB
• Tumor rejection antigen AB
• tyrosinase (oculocutaneous albinism IA)
• tyrosinase

Background

The enzyme encoded by this gene catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this gene result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3' half of this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: TYR
• Uniprot:
  • P14679(Human)