Tyrosinase Antibody (SPM360) [DyLight 550]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC-Fr, IHC-P, IP
Clone
SPM360
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 550

Tyrosinase Antibody (SPM360) [DyLight 550] Summary

Immunogen
Recombinant tyrosinase protein
Localization
Cytoplasmic
Marker
Melanoma Marker
Specificity
Recognizes a cluster of proteins between 70-80kDa, identified as tyrosinase. Occasionally a minor band at 55kDa is also detected. This MAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this MAb shows tyrosinase in melanotic as well as amelanotic variants. This MAb is a useful marker for melanocytes and melanomas.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TYR
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Tyrosinase Antibody (SPM360) [DyLight 550]

  • CMM8
  • EC 1.14.18.1
  • LB24-AB
  • Monophenol monooxygenase
  • OCA1A
  • OCAIA
  • SHEP3
  • SK29-AB
  • Tumor rejection antigen AB
  • tyrosinase (oculocutaneous albinism IA)
  • tyrosinase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Tyrosinase Antibody (NBP2-34770R) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NBP2-34770AF405
Alexa Fluor 488 NBP2-34770AF488
Alexa Fluor 647 NBP2-34770AF647
Alexa Fluor 700 NBP2-34770AF700
Allophycocyanin NBP2-34770APC
Biotin NBP2-34770B
DyLight 350 NBP2-34770UV
DyLight 405 NBP2-34770V
DyLight 488 NBP2-34770G
DyLight 550 NBP2-34770R
DyLight 650 NBP2-34770C
DyLight 680 NBP2-34770FR
DyLight 755 NBP2-34770IR
HRP NBP2-34770H
Janelia Fluor 549 NBP2-34770JF549
Janelia Fluor 646 NBP2-34770JF646
PE NBP2-34770PE
PerCP NBP2-34770PCP

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Bioinformatics Tool for Tyrosinase Antibody (NBP2-34770R)

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Contact Information

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Calculators

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Bioinformatics

Gene Symbol TYR