TXNDC5 Antibody - BSA Free

Western Blot: TXNDC5 Antibody [NBP2-98558] - Anti-TXNDC5 rabbit polyclonal antibody at 1:5000 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: HEK293 Whole Cell Lysate Lane D: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 48 kDa. Observed band size: 50 kDa

Immunohistochemistry-Paraffin: TXNDC5 Antibody [NBP2-98558] - Immunochemical staining of human TXNDC5 in human tonsil with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: TXNDC5 Antibody [NBP2-98558] - Immunochemical staining of human TXNDC5 in human stomach with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: TXNDC5 Antibody [NBP2-98558] - TXNDC5 was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 4 ul anti-TXNDC5 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-TXNDC5 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 48 kDa. Observed band size: 50 kDa

Immunocytochemistry/Immunofluorescence: TXNDC5 Antibody [NBP2-98558] - Immunofluorescence staining of TXNDC5 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98558 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Cytoplasm.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TXNDC5 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human TXNDC5 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TXNDC5
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry-Paraffin 1:1000-1:10000
  • Immunoprecipitation 0.5-1uL/mg of lysate
  • Western Blot 1:5000-1:20000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for TXNDC5 Antibody - BSA Free

• EndoPDI
• ERp46
• FLJ90810
• HCC-2
• member 15
• MGC3178
• PDIA15
• thioredoxin domain containing 5 (endoplasmic reticulum)
• thioredoxin domain containing 5
• thioredoxin domain-containing protein 5
• thioredoxin related protein
• TLP46
• TXNDC5

Background

TXNDC5 encodes a protein-disulfide isomerase. Its expression is induced by hypoxia and its role may be to protect hypoxic cells from apoptosis. This gene can be co-transcribed with the upstream gene MU.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TXNDC5