Recombinant Human CCL23/Ck beta 8-1 (aa 46-137) Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human CCL23/Ck beta 8-1 (aa 46-137) Protein, CF Summary

Details of Functionality
Measured by its ability to chemoattract THP‑1 human acute monocytic leukemia cells. The ED50 for this effect is 0.01‑0.04 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR1. The ED50 for this effect is 0.002‑0.01 µg/mL.
Source
E. coli-derived human CCL23/Ck beta 8-1 protein
Met46-Asn137
Accession #
N-terminal Sequence
Met46
Protein/Peptide Type
Recombinant Proteins
Gene
CCL23
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
10.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
508-CK/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CCL23/Ck beta 8-1 (aa 46-137) Protein, CF

  • C6 beta-chemokine
  • CCL23/Ck beta 8-1
  • chemokine (C-C motif) ligand 23
  • Ck beta 8-1
  • CKb8
  • Ckb-8
  • Ckb81
  • CK-beta-8
  • hmrp-2a
  • Macrophage inflammatory protein 3
  • MIP3
  • MIP-3CK-BETA-8
  • MPIF1
  • MPIF-1Ckb-8-1
  • Myeloid progenitor inhibitory factor 1
  • SCYA23C-C motif chemokine 23
  • small inducible cytokine subfamily A (Cys-Cys), member 23
  • Small-inducible cytokine A23

Background

CCL23 (SCY A23), a splice variant of CK beta 8 (also known as MPIF-1), is a beta chemokine isolated from the THP-1 cDNA library. CCL23 cDNA encodes a 137 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide. Compared to CK beta 8-1, CK beta 8/MPIF-1 lacks a 17 aa residue stretch (Leu 47 - Gly 63) present in CCL23 and has a unique arginine at residue 46. CK beta 8-1/CK beta 8 (MPIF-1) and leukotactin/MIP-1δ form a subgroup of beta chemokines that have 6 conserved cysteine residues and an extended amino-terminus preceding the conserved cysteine pair. CCL23 shares approximately 73% nucleotide sequence identity with leukotactin (MIP-1δ), and the two genes have been localized to human chromosome 17 within 200 kb of each other. CCL23 mRNA expression has been detected in the pancreas, heart and skeletal muscle. Both CK beta 8-1 and CK beta 8 (MPIF-1) are potent agonists of CCR1 and have been shown to chemoattract peripheral blood lymphocytes and monocytes. The two chemokines have also been reported to chemoattract neutrophils and to inhibit colony formation by human hematopoietic progenitor cells. Similar to CK beta 8 (MPIF-1), the truncated form of CK beta 8-1 (aa 46 - 132) produced at R&D Systems has been shown to have greatly enhanced biological activity compared to the full length mature CK beta 8-1 (aa 22 - 132).

  1. Youn, B-S. et al. (1998) Blood 91:3118.
  2. Macphee, C.H. et al. (1998) J. Immunol. 161:6273.

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Publications for CCL23/Ck beta 8-1/MIP3 (508-CK/CF)(4)

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Bioinformatics

Gene Symbol CCL23
Entrez
Uniprot