CCL15/MIP-1 delta Antibody (52130) [Unconjugated]

Recombinant Human CCL15/MIP‑1δ (Catalog # 363-MG) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL15/MIP‑1δ (16 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL15/MIP‑1δ Mono­clonal Antibody (Catalog # MAB363). The ND50 is typically 3-15 µg/mL.

• Reactivity: Hu
• Applications: WB, B/N, ELISA(Cap)
• Clone: 52130
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CCL15/MIP-1 delta Antibody (52130) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CCL15/MIP-1δ
  Gln22-Ile113
  Accession # Q16663.2
• Specificity: Detects human CCL15/MIP‑1δ in ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCL15
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Neutralization 3-15 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Detection: Human CCL15/MIP-1delta Biotinylated Antibody (Catalog number BAF363)
  Standard: Recombinant Human CCL15/MIP-1delta 92 aa (Catalog number 363-MG)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL15/MIP-1 delta Antibody (52130) [Unconjugated]

• CCL15
• chemokine (C-C motif) ligand 15
• Chemokine CC-2
• HCC-2
• HCC-2Lkn-1
• Leukotactin 1
• Leukotactin-1
• LKN1
• LKN-1
• Macrophage inflammatory protein 5
• MIP1 delta
• MIP-1 delta
• MIP5
• MIP-5
• MIP-5MIP-1d
• Mrp-2b
• NCC-3
• NCC-3MIP-1D
• NCC3MRP-2B
• new CC chemokine 3
• SCYA15member 15
• Small-inducible cytokine A15

Background

CCL15, a human CC chemokine, was isolated from a human fetal spleen cDNA library. CCL15 cDNA encodes a predicted 113 amino acid (aa) protein containing a putative signal peptide of 21 aa that is cleaved to generate a 92 aa residue mature protein. Within the CC family members, human CCL15 shares 45%, 44%, 35%, and 30% aa homology with mouse C10, human MPIF-1, human HCC-1, and mouse MIP-1 gamma, respectively. The gene for MIP-1δ is found on chromosome 17 where the genes for most of the human CC chemokines are located. Human CCL15 is expressed in T and B lymphocytes, NK cells, monocytes and monocyte-derived dendritic cells. Human MIP-1δ is chemotactic for T cells and monocytes and has been shown to induce calcium flux in human CCR-1-transfected cells.

1. Wang, W. et al. (1998) J. Clinical Immunol. 18:214

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL15/MIP-1 delta Antibody (MAB363)(3)

Publications using MAB363

• Joubert P, Lajoie-Kadoch S, Wellemans V, Létuvé S, Tulic MK, Halayko AJ, Hamid Q Expression and regulation of CCL15 by human airway smooth muscle cells. Clin. Exp. Allergy, 2011;42(1):85-94. 2011 [PMID: 22092970] (ICC, Human)
• Hu H, Kwun J, Aizenstein BD, Knechtle SJ Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Transplantation, 2009;87(12):1814-20. 2009 [PMID: 19543058] (Antibody Array Development, Human)
• Haringman JJ, Smeets TJ, Reinders-Blankert P Chemokine and chemokine receptor expression in paired peripheral blood mononuclear cells and synovial tissue of patients with rheumatoid arthritis, osteoarthritis, and reactive arthritis. Ann. Rheum. Dis., 2006;65(3):294-300. 2006 [PMID: 16107514] (IHC-Fr, Human)

Bioinformatics

• Gene Symbol: CCL15
• Uniprot:
  • Q16663.2()