TWEAK/TNFSF12 Antibody

Images

 
Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Analysis of TWEAK in HeLa cell lysate with TWEAK antibody at (A) 1 and (B) 2 ug/mL.
Immunohistochemistry: TWEAK/TNFSF12 Antibody [NBP1-76695] - Staining of TWEAK in mouse brain tissue with TWEAK antibody at 5 ug/mL.
Immunohistochemistry-Paraffin: TWEAK/TNFSF12 Antibody [NBP1-76695] - Human brain tissue with TWEAK antibody at 10 ug/ml.
Immunofluorescence: TWEAK/TNFSF12 Antibody [NBP1-76695] - Mouse brain tissue with TWEAK antibody at 20 ug/mL.Red: TWEAK Antibody (3791) Blue: DAPI staining

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

TWEAK/TNFSF12 Antibody Summary

Immunogen
Antibody was raised against recombinant human TWEAK protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TNFSF12
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 2 ug/mL
  • ELISA 1:100-1:2000
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunofluorescence 20 ug/ml
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-76695 in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for TWEAK/TNFSF12 Antibody

  • APO3 ligand
  • APO3LMGC20669
  • DR3LGTWEAKAPO3/DR3 ligand
  • MGC129581
  • TNF-related weak inducer of apoptosis
  • TNFSF12
  • tumor necrosis factor (ligand) superfamily, member 12
  • tumor necrosis factor ligand superfamily member 12
  • TWEAK

Background

TNF-related weak inducer of apoptosis (TWEAK) is a member of the tumor necrosis factor superfamily (TNFSF) of structurally related cytokines. Like most other members of this family, TWEAK is a cell surface-associated type II transmembrane protein although a smaller, biologically active form can also be generated by cleavage near the cell membrane. TWEAK has multiple biological activities, including stimulation of cell growth and angiogenesis, induction of inflammatory cytokines, in addition to stimulation of apoptosis. The TWEAK signal transduction pathway has not been well established but it appears to signal via TweakR/Fn14 in a manner similar to that described for other TNFSF members that bind receptors lacking death domains. Despite its predicted molecular weight of 27kDa, TWEAK may migrate at aberrant positions in SDS-PAGE.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TWEAK/TNFSF12 Antibody (NBP1-76695)(2)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 2 applications: IHC, WB.


Filter By Application
IHC
(1)
WB
(1)
All Applications
Filter By Species
Mouse
(1)
Rat
(1)
All Species

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for TWEAK/TNFSF12 Antibody (NBP1-76695). (Showing 1 - 1 of 1 FAQs).

  1. We recently purchased a TWEAK antibody (NBP1-76695) from you to use in Western blot. I notice that it has an ELISA application; can we biotinylate this antibody to use it as a detection agent in ELISA or can you suggest an appropriate detection antibody?
    • This antibody can certainly be conjugated directly to biotin. We have Lightning-Link kits that can do this for you because of the buffer and purification of this one. As far as detection antibodies are concerned, you could use a secondary against Rabbit IgG conjugated to biotin as your detection if that is what you would prefer to do.

Secondary Antibodies

 

Isotype Controls

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Diseases for TWEAK/TNFSF12 Antibody (NBP1-76695)

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PTMs for TWEAK/TNFSF12 Antibody (NBP1-76695)

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Bioinformatics

Gene Symbol TNFSF12
Entrez
Uniprot