| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 1090-TW-025/CF | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 1237-TW-025/CF | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76695-0.025mg | |||
1090-TW) inhibits proliferation in the the HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line). Activity elicited by Recombinant Human TWEAK/TNFSF12 (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TWEAK/ TNFSF12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1090). The ND50 is typically 2-8 µg/mL." alt="Recombinant Human TWEAK/TNFSF12 (Catalog # 1090-TW) inhibits proliferation in the the HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line). Activity elicited by Recombinant Human TWEAK/TNFSF12 (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TWEAK/ TNFSF12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1090). The ND50 is typically 2-8 µg/mL." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1090-SP | |||
1237-TW) inhibits proliferation in the the HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line). Activity elicited by Recombinant Mouse TWEAK/TNFSF12 (2 µg/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Mouse TWEAK/ TNFSF12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1237). The ND50 is typically 4-16 µg/mL." alt="Recombinant Mouse TWEAK/TNFSF12 (Catalog # 1237-TW) inhibits proliferation in the the HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line). Activity elicited by Recombinant Mouse TWEAK/TNFSF12 (2 µg/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Mouse TWEAK/ TNFSF12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1237). The ND50 is typically 4-16 µg/mL." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1237-SP | |||
1237-TW) was serially diluted and captured by Rat Anti-Mouse TWEAK/TNFSF12 Monoclonal Antibody (Catalog # MAB1237) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse TWEAK/TNFSF12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1237) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Mouse TWEAK/TNFSF12 (Catalog # 1237-TW) was serially diluted and captured by Rat Anti-Mouse TWEAK/TNFSF12 Monoclonal Antibody (Catalog # MAB1237) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse TWEAK/TNFSF12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1237) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1237-SP | |||
1090-TW) was serially diluted and captured by Mouse Anti-Human TWEAK/TNFSF12 Monoclonal Antibody (Catalog # MAB1090) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TWEAK/TNFSF12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1090) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human TWEAK/TNFSF12 (Catalog # 1090-TW) was serially diluted and captured by Mouse Anti-Human TWEAK/TNFSF12 Monoclonal Antibody (Catalog # MAB1090) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TWEAK/TNFSF12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1090) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1090-SP | |||
| Carrier Free | MAB1090-500 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-46811-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09045-0.025mg | |||
![N/A TWEAK/TNFSF12 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_TWEAK_DY1090_ELISA_1569.jpg "N/A TWEAK/TNFSF12 [Biotin]")
![Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Western Blot Validation in Mouse Liver Tissue Lysates. Loading: 15 ug of lysates per lane. Antibodies: TWEAK/TNFSF12 NBP1-76695 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/TWEAK-TNFSF12-Antibody-Western-Blot-NBP1-76695-img0011.jpg "Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Western Blot Validation in Mouse Liver Tissue Lysates. Loading: 15 ug of lysates per lane. Antibodies: TWEAK/TNFSF12 NBP1-76695 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Analysis of TWEAK in HeLa cell lysate with TWEAK antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/TWEAK-TNFSF12-Antibody-Western-Blot-NBP1-76695-img0004.jpg)
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human TWEAK recombinant protein per lane. Antibodies: TWEAK/TNFSF12 NBP1-76695 (Lane 1: 0.5 ug/mL, Lane 2: 1 ug/mL, Lane 3: 2 ug/mL and Lane 4: 4 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 18 kDa.](http://images.novusbio.com/fullsize/TWEAK-TNFSF12-Antibody-Western-Blot-NBP1-76695-img0009.jpg)
![Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Western Blot Validation in Mouse Pancreas Tissue Lysates. Loading: 15 ug of lysates per lane. Antibodies: TWEAK/TNFSF12 NBP1-76695 (Lane 1: 1 ug/mL and Lane 2: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/TWEAK-TNFSF12-Antibody-Western-Blot-NBP1-76695-img0010.jpg)
![Immunohistochemistry: TWEAK/TNFSF12 Antibody - BSA Free [NBP1-76695] - Validation of TWEAK/TNFSF12 in Human Brain Tissue. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-TWEAK/TNFSF12 antibody at 10 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76695_rabbit-polyclonal-tweak-tnfsf12-antibody-2711202420115594.jpg)
![Immunohistochemistry: TWEAK/TNFSF12 Antibody - BSA Free [NBP1-76695] - Validation of TWEAK/TNFSF12 in Mouse Brain Tissue. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TWEAK/TNFSF12 antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76695_rabbit-polyclonal-tweak-tnfsf12-antibody-2711202420272466.gif)
![Immunocytochemistry/ Immunofluorescence: TWEAK/TNFSF12 Antibody - BSA Free [NBP1-76695] - Validation of TWEAK/TNFSF12 in Mouse Brain Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain tissue labeling TWEAK/TNFSF12 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76695_rabbit-polyclonal-tweak-tnfsf12-antibody-2711202419514021.gif)
NL001) and counterstained with DAPI (blue). View our protocol for 
1090-TW) was serially diluted and captured by Mouse Anti-Human TWEAK/TNFSF12 Monoclonal Antibody (Catalog # ![ELISA: Human TWEAK/TNFSF12 ELISA Kit (Colorimetric) [NBP3-06808] - Samples were spiked with high concentrations of Human TWEAK/TNFSF12 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-06808_human-tweak-tnfsf12-elisa-kit-colorimetric-132202416254062.jpg "ELISA: Human TWEAK/TNFSF12 ELISA Kit (Colorimetric) [NBP3-06808] - Samples were spiked with high concentrations of Human TWEAK/TNFSF12 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human TWEAK/TNFSF12 ELISA Kit (Colorimetric) [NBP3-06808] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-06808_human-tweak-tnfsf12-elisa-kit-colorimetric-4320249124886.jpg)
![N/A TWEAK/TNFSF12 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_TWEAK_DY1237_ELISA_1609.jpg "N/A TWEAK/TNFSF12 [Biotin]")
1237-TW) was serially diluted and captured by Rat Anti-Mouse TWEAK/TNFSF12 Monoclonal Antibody (Catalog # ![Immunohistochemistry: TWEAK/TNFSF12 Antibody [NBP2-46811] - Staining of human skin shows strong cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/antibody/nbp2-46811_rabbit-polyclonal-tweak-tnfsf12-antibody-immunohistochemistry-paraffin-148202413756..jpg "Immunohistochemistry: TWEAK/TNFSF12 Antibody [NBP2-46811] - Staining of human skin shows strong cytoplasmic positivity in squamous epithelial cells.")
![Immunohistochemistry: TWEAK/TNFSF12 Antibody [NBP2-46811] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/antibody/nbp2-46811_rabbit-polyclonal-tweak-tnfsf12-antibody-immunohistochemistry-paraffin-14820241392..jpg)
![Immunohistochemistry: TWEAK/TNFSF12 Antibody [NBP2-46811] -Staining of human rectum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp2-46811_rabbit-polyclonal-tweak-tnfsf12-antibody-immunohistochemistry-paraffin-148202413955..jpg)
![Immunohistochemistry: TWEAK/TNFSF12 Antibody [NBP2-46811] -Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/antibody/nbp2-46811_rabbit-polyclonal-tweak-tnfsf12-antibody-immunohistochemistry-paraffin-1482024131057..jpg)
![Immunocytochemistry/Immunofluorescence: TWEAK/TNFSF12 Antibody (MTW-1) - Chimeric [NBP3-09045] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of MTW-1 (NBP3-09045) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09045, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/TWEAK-TNFSF12-Antibody-MTW-1-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09045-img0001.jpg "Immunocytochemistry/Immunofluorescence: TWEAK/TNFSF12 Antibody (MTW-1) - Chimeric [NBP3-09045] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of MTW-1 (NBP3-09045) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09045, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.")
