TWEAK R/TNFRSF12 Antibody (ITEM-4) - Low Endotoxin

Flow Cytometry: TWEAK R/TNFRSF12 Antibody (ITEM-4) - Low Endotoxin, Azide and BSA Free [NBP3-09099] - Separation of HUVEC cells stained using anti-human CD266 (ITEM-4) purified antibody (low endotoxin, concentration in sample 1 ug/ml, GAM APC, red-filled) from HUVEC cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Flow Cytometry: TWEAK R/TNFRSF12 Antibody (ITEM-4) - Low Endotoxin, Azide and BSA Free [NBP3-09099] - Surface staining pattern of HUVEC cells stained using anti-human CD266 (ITEM-4) purified antibody (low endotoxin, concentration in sample 1 ug/ml) GAM APC.

• Reactivity: Hu, Mu
• Applications: WB, Flow, Func, IHC
• Clone: ITEM-4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Low Endotoxin
• Concentration: 1 mg/ml

TWEAK R/TNFRSF12 Antibody (ITEM-4) - Low Endotoxin Summary

• Description: Novus Biologicals Mouse TWEAK R/TNFRSF12 Antibody (ITEM-4) - Low Endotoxin (NBP3-09099) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: human TWEAK R/TNFRSF12-transfected P815 cells
• Specificity: The mouse monoclonal antibody ITEM-4 recognizes an extracellular epitope of CD266 / TWEAK R, a TNFR superfamily receptor for CD255 / TWEAK, a TNF-like weak inducer of apoptosis.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFRSF12A
• Purity: Protein A purified
• Endotoxin Note: Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-4 ug/ml
  • Functional
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for TWEAK R/TNFRSF12 Antibody (ITEM-4) - Low Endotoxin

• CD266 antigen
• CD266
• FGF-inducible 14
• Fibroblast growth factor-inducible immediate-early response protein 14
• Fn14
• FN14tumor necrosis factor receptor superfamily member 12A
• TNFRSF12
• TNFRSF12A
• tumor necrosis factor receptor superfamily, member 12A
• TWEAK R
• TWEAKR
• tweak-receptor
• type I transmembrane protein Fn14

Background

TWEAK (otherwise known as TNFRSF12A, Tumor necrosis factor receptor superfamily member 12A), is a member of the TNF family and was first described as a weak inducer of apoptosis in some cell types. It is thought to promote angiogenesis and the proliferation of endothelial cells as well as modulating cellular adhesion to matrix proteins. Recently, a receptor for TWEAK was isolated by expression cloning from a HUVEC cell cDNA library.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNFRSF12A