TWEAK R/TNFRSF12 Antibody (314502)

• Reactivity: Hu
• Applications: WB
• Clone: 314502
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

TWEAK R/TNFRSF12 Antibody (314502) Summary

• Immunogen: E. coli-derived recombinant human TWEAK R/TNFRSF12
  Glu28-Trp79
  Accession # Q9NP84.1
• Specificity: Detects human TWEAK R/TNFRSF12 in direct ELISAs and Western blots. Shows approximately 40% cross-reactivity with recombinant mouse TWEAK R and no cross-reactivity with recombinant human (rh) OPG or rhTNF RI.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: TNFRSF12A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TWEAK R/TNFRSF12 Antibody (314502)

• CD266 antigen
• CD266
• FGF-inducible 14
• Fibroblast growth factor-inducible immediate-early response protein 14
• Fn14
• FN14tumor necrosis factor receptor superfamily member 12A
• TNFRSF12
• TNFRSF12A
• tumor necrosis factor receptor superfamily, member 12A
• TWEAK R
• TWEAKR
• tweak-receptor
• type I transmembrane protein Fn14

Background

The gene for TNF-related weak inducer of apoptosis receptor (TWEAK R) was originally identified as a fibroblast growth factor-inducible immediate-early response gene Fn14 in mouse NIH 3T3 fibroblasts (1, 2). Human TWEAK R cDNA encodes a 129 amino acid (aa) residue type I transmembrane protein with a 27 aa signal peptide, a 53 aa extracellular domain, a 21 aa transmembrane domain and a 28 aa cytoplasmic domain (1-3). Human and mouse TWEAK R share 82% aa sequence identity. TWEAK R is the smallest member of the TNF receptor superfamily and contains only one cysteine-rich region in its extracellular domain. The TWEAK R cytoplasmic domain conatins one TRAF binding motif which binds TRAFs 1, 2, and 3. TWEAK R binds its ligand TWEAK/TNFSF12 with high affinity to initiate a signal transduction cascade that depending upon the cell type, may lead to a variety of cellular responses including cell death, cell proliferation, and angiogenesis (2-6). In new born mice, TWEAK R is highly expressed in all tissues examined (heart, intestine, kidney, liver, lung and skin) (1). In adult mice, high TWEAK R expression levels are found in the heart and ovary, while lower expression levels are detected in the lung, kidney, skin. Elevated levels of TWEAK R mRNA were found in human or mouse hepatocellular carcinoma specimens, in regenerating mouse liver and in injured rat arteries (2, 3).

1. Meighan-Mantha, R. et al. (1999) J. Biol. Chem. 274:33166.
2. Feng, S. et al. (2000) Am J. Pathol. 156:1253.
3. Wiley, S. et al. (2001) Immunity 15:837.
4. Schneider, P. et al. (1999) Eur. J. Immunol. 29:1785.
5. Nakayama, M. et al. (2002) J. Immunol. 168:734.
6. Lynch, C.N. et al. (1999) J. Biol. Chem. 274:8455.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TWEAK R/TNFRSF12 Antibody (MAB1199)(2)

Publications using MAB1199

• T Song, D Spillmann Transcriptomic analysis reveals cell apoptotic signature modified by heparanase in melanoma cells J. Cell. Mol. Med., 2019-05-02;0(0):. 2019-05-02 [PMID: 31044520]
• Tiziana Benicchi, Sara Iozzi, Andreas Svahn, Hanna Axelsson, Elisa Mori, Simonetta Bernocco et al. A Homogeneous HTRF Assay for the Identification of Inhibitors of the TWEAK-Fn14 Protein Interaction J Biomol Screen 2012-08-01 [PMID: 22644269]

Bioinformatics

• Gene Symbol: TNFRSF12A
• Uniprot:
  • Q9NP84.1()