TSPAN3 Antibody

Western Blot: Tetraspanin-3 Antibody [NBP2-20604] - Sample (50 ug of whole cell lysate) A: Mouse Kidney, 12% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Tetraspanin-3 Antibody [NBP2-20604] - HeLa cells. Green: TSPAN3 protein stained by TSPAN3 antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: TSPAN3 Antibody [NBP2-20604] - U87 xenograft. TSPAN3 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: Tetraspanin-3 Antibody [NBP2-20604] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 12% SDS PAGE gel, diluted at 1:1000.

TSPAN3 antibody detects TSPAN3 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: TSPAN3 protein stained by TSPAN3 antibody (NBP2-20604) diluted at 1:500.Blue: Hoechst 33342 staining.

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with TSPAN3 antibody (NBP2-20604) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

TSPAN3 Antibody Summary

• Description: Novus Biologicals Rabbit TSPAN3 Antibody (NBP2-20604) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human TSPAN3. The exact sequence is proprietary.
• Localization: Membrane; Multi-pass membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TSPAN3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for TSPAN3 Antibody

• tetraspan TM4SF
• tetraspanin 3
• Tetraspanin TM4-A
• tetraspanin-3
• TM4A
• TM4-A
• TM4SF8
• Transmembrane 4 superfamily member 8tetraspan 3
• TSPAN3
• TSPAN-3

Background

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The use of alternate polyadenylation sites has been found for this gene. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: TSPAN3