TSLP Antibody - BSA Free

Western Blot: TSLP Antibody [NBP1-76754] - A-20 cell lysate with TSLP antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.

Immunohistochemistry-Paraffin: TSLP Antibody [NBP1-76754] - Mouse brain tissue with TSLP antibody at 2.5 ug/ml.

Immunocytochemistry/ Immunofluorescence: TSLP Antibody - BSA Free [NBP1-76754] - Figure 2 Immunofluorescence Validation of TSLP in Mouse Brain TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed Mouse Brain Tissue labeling TSLP with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

• Reactivity: Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TSLP Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 20 amino acid synthetic peptide from near the center of mouse TSLP. The immunogen is located within amino acids 70 - 120 of TSLP.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TSLP
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 0.5-2 ug/ml
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  TSLP Peptide (NBP1-76754PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for TSLP Antibody - BSA Free

• thymic stromal lymphopoietin
• TSLP

Background

Thymic stromal lymphopoietin (TSLP) has recently been identified as an important factor capable of driving dendritic cell maturation and activation. TSLP is a four-helix-bundle cytokine that is expressed mainly by barrier epithelial cells and is a potent activator of several cell types such as myeloid dendritic cells. TSLP is involved in the positive selection of regulatory T cells, maintenance of peripheral CD4+ T cell homeostasis and the induction of CD4+ T cell-mediated allergic reaction. TSLP is also capable of supporting the growth of fetal liver and adult B cell progenitors and their differentiation to the IgM-positive stage of B cell development. Amino acid sequence analysis has shown poor homology between human and mouse TSLP although they exhibit similar biological functions and are expressed in similar tissues. Despite its predicted molecular weight, TSLP often migrates at a higher molecular weight in SDS-PAGE. At least two differentially spliced isoforms of TSLP are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TSLP Antibody (NBP1-76754)(1)

Publication using NBP1-76754

• Sozmen SC, Karaman M, Micili SC et al. Resveratrol ameliorates 2, 4-dinitrofluorobenzene-induced atopic dermatitis-like lesions through effects on the epithelium. PeerJ. 2016-03-31 [PMID: 27069818] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: TSLP
• Uniprot:
  • NP_067342()