TSLP Antibodies: Novus Biologicals

AF555

TSLP Antibody [Unconjugated]

100 μg

AF555

555-TS) stimulates proliferation in the BaF3 mouse pro‑B cell line transfected with mouse IL‑7 Ra/CD127 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse TSLP (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse TSLP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF555). The ND₅₀ is typically ≤0.4 µg/mL." class="big_lightbox">

555-TS) stimulates proliferation in the BaF3 mouse pro‑B cell line transfected with mouse IL‑7 Ra/CD127 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse TSLP (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse TSLP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF555). The ND₅₀ is typically ≤0.4 µg/mL." alt="Recombinant Mouse TSLP (555-TS) stimulates proliferation in the BaF3 mouse pro‑B cell line transfected with mouse IL‑7 Ra/CD127 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse TSLP (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse TSLP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF555). The ND₅₀ is typically ≤0.4 µg/mL." class="big_thumb" />

Klk5 deletion results in remarkable suppression of the inflammatory phenotype of NS.(A-E). Results show remarkable abrogation of pro-allergic and pro-inflammatory cytokines quantified by RT-qPCR. (A) Il-1 beta , Tslp, Tnf-alpha ; (B) Il-6, Il-18, the type 17 promoting cytokine Il-23p19; (C) Ifn-gamma , Il-4, Il-17A, Il-22; (D) Th17 regulated genes Defb4, Slpi, Cxcl1 and Ccl20; (E) Th17/22 regulated genes s100a7, s100a8, s100a9. Data are shown as the mean ± s.e.m. of triplicate amplification for at least five mice per genotype. Results are normalized to wt mean (set as 1.0). *p<0.05, **p<0.005, ***p<0.001 (Mann-Whitney U test). (F) Quantification of mast cells based on toluidine blue staining of skin tissue (mast cells stain purple). An increased number of mast cells was detected in Spink5-/- dermis; (G) IHC analysis shows abolishment of Tslp expression in Spink5-/-Klk5-/- skin as compared to Spink5-/-; Scale bar: 50μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26390218), licensed under a CC-BY license. Not internally tested by R&D Systems.

555-TS) was serially diluted and captured by Rat Anti-Mouse TSLP Monoclonal Antibody (Catalog # MAB5551) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse TSLP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF555) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Mouse TSLP (Catalog # <a class=

555-TS) was serially diluted and captured by Rat Anti-Mouse TSLP Monoclonal Antibody (Catalog # MAB5551) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse TSLP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF555) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF555U-100UG
Alexa Fluor 405	AF555V-100UG
Alexa Fluor 488	AF555G-100UG
Alexa Fluor 532	AF555X-100UG
Alexa Fluor 594	AF555T-100UG
Alexa Fluor 647	AF555R-100UG
Alexa Fluor 700	AF555N-100UG
Alexa Fluor 750	AF555S-100UG
Alexa Fluor Plus 405	AF555AFP405-100UG
Alexa Fluor Plus 488	AF555AFP488-100UG
Alexa Fluor Plus 555	AF555AFP555-100UG
Alexa Fluor Plus 594	AF555AFP594-100UG
Alexa Fluor Plus 647	AF555AFP647-100UG
Alexa Fluor Plus 680	AF555AFP680-100UG
Allophycocyanin	AF555A
Biotin	BAF555
CoraFluor 1	AF555CL1
DyLight 350	AF555D
DyLight 405	AF555E
DyLight 488	AF555K
DyLight 550	AF555L
DyLight 594	AF555M
DyLight 650	AF555W
DyLight 680	AF555Y
DyLight 755	AF555Z
FITC	AF555F
HRP	AF555H
Janelia Fluor 525	AF555JF525
Janelia Fluor 549	AF555I
Janelia Fluor 646	AF555J
Janelia Fluor 669	AF555JF669
PE	AF555P
PerCP	AF555C
mFluor Violet 450 SE	AF555MFV450
mFluor Violet 500 SE	AF555MFV500
mFluor Violet 610 SE	AF555MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF555-SP

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MAB555

TSLP Antibody (152614)

(25 Publications)

100 μg

MAB555

555-TS) stimulates proliferation in the BaF3 mouse pro‑B cell line transfected with mouse IL‑7 Ra in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse TSLP (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse TSLP Monoclonal Antibody (Catalog # MAB555). The ND₅₀ is typically 0.1-0.4 µg/mL." class="big_lightbox">

555-TS) stimulates proliferation in the BaF3 mouse pro‑B cell line transfected with mouse IL‑7 Ra in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse TSLP (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse TSLP Monoclonal Antibody (Catalog # MAB555). The ND₅₀ is typically 0.1-0.4 µg/mL." alt="Recombinant Mouse TSLP (Catalog # 555-TS) stimulates proliferation in the BaF3 mouse pro‑B cell line transfected with mouse IL‑7 Ra in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse TSLP (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse TSLP Monoclonal Antibody (Catalog # MAB555). The ND₅₀ is typically 0.1-0.4 µg/mL." class="big_thumb" />

<b>Biological Strategies Validation. </b>TSLP neutralization inhibits airway structural alterations.(A) The depth of peribronchial collagen deposition was significantly reduced in anti-TSLP-treated mice, compared with HDM-exposed and IgG isotype-treated controls. (B) Epithelial goblet cells were markedly reduced in anti-TSLP-treated mice, compared to the HDM-exposed and IgG -treated controls. (C) TGF-beta 1 levels in the BALF were significantly increased in HDM-exposed mice. The blockage of TSLP with the anti-TSLP mAb reduced TGF-beta 1levels in the BALF. TGF-beta 1 protein and mRNA levels were determined in duplicate experiments. (D-G) Representative photomicrographs of Masson's trichrome-stained lung sections from mice that were exposed for 5 weeks to saline (D) or HDM (E) and pretreated with anti-TSLP mAb (F) or an IgG-treated control (G) 60 minutes prior to and during HDM exposure. (H-K) Representative photomicrographs of airway sections of PAS-stained from mice that were exposed for 5 weeks to saline (H) or HDM (I) and pretreated with anti-TSLP mAb (J) or an IgG-treated control (K) 60 minutes prior to and during the HDM exposure. Original magnification, 20×. The data shown represent the means±SEM (n = 5), **p<0.01 compared with the HDM group, ##p<0.01 compared with the IgG-treated control mice. The photomicrographs of H-K are representative of 5 independent experiments. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0051268), licensed under a CC-BY license. Not internally tested by R&D Systems.

TSLP neutralization inhibits surface marker expression on airway CD11c+ cells.(A) Lung cell suspensions from mice were stained with combinations of PerCP-Cy 5.5-conjugated CD11c, PE-conjugated OX40L, FITC-conjugated CD80 and APC-conjugated CD86. The dead cells and the debris were excluded based on forward scatter (FSC)/side scatter (SSC) plots. (B) A total of 1×105 to 3×105 viable cells were acquired using FACS, and the DCs were then sorted as CD11c+ airway cells on FSC/SSC plots. (C) Mice that were chronically exposed to HDM received an intranasal administration of anti-TSLP mAb or of a control IgG 60 minutes prior to each HDM exposure. The expression levels of OX40L, CD80 and CD86 on pulmonary DCs were then analyzed using flow cytometry. The HDM-exposed mice exhibited a significant increase in the OX40L, CD80 and CD86 surface markers on CD11c+ airway cells. However, anti-TSLP pretreatment effectively reduced OX40L, CD80 and CD86 expression on the DCs, even though the mice were continuously exposed to HDM. (D) The TSLP levels in the BALF were highly correlated with OX40L expression on CD11c+ airway cells in all of the treatment mice (n = 20, R = 0.879, p<0.01). (E) Representative data from one of the five replicates. (F) Staining for OX40L (12-4031), CD80 (11-4888), and CD86 (17-4321) from isotype-Ig-treated controls. The data represent the means±SEM (n = 5). * p<0.05 or ** p<0.01 compared with the HDM group. # p<0.05 or ## p<0.01 compared with the IgG-treated control mice. The results are derived from 4 experimental groups (5 mice/per group), and the data are representative of 5 independent experiment. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23300949), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB555U-100UG
Alexa Fluor 405	FAB555V-100UG
Alexa Fluor 488	FAB555G-100UG
Alexa Fluor 532	FAB555X-100UG
Alexa Fluor 594	FAB555T-100UG
Alexa Fluor 647	FAB555R-100UG
Alexa Fluor 700	FAB555N-100UG
Alexa Fluor 750	FAB555S-100UG
Alexa Fluor Plus 405	FAB555AFP405-100UG
Alexa Fluor Plus 488	FAB555AFP488-100UG
Alexa Fluor Plus 555	FAB555AFP555-100UG
Alexa Fluor Plus 594	FAB555AFP594-100UG
Alexa Fluor Plus 647	FAB555AFP647-100UG
Alexa Fluor Plus 680	FAB555AFP680-100UG
Allophycocyanin	FAB555A
Biotin	FAB555B
CoraFluor 1	FAB555CL1
DyLight 350	FAB555D
DyLight 405	FAB555E
DyLight 488	FAB555K
DyLight 550	FAB555L
DyLight 594	FAB555M
DyLight 650	FAB555W
DyLight 680	FAB555Y
DyLight 755	FAB555Z
FITC	FAB555F
HRP	FAB555H
Janelia Fluor 525	FAB555JF525
Janelia Fluor 549	FAB555I
Janelia Fluor 646	FAB555J
Janelia Fluor 669	FAB555JF669
PE	FAB555P
PerCP	FAB555C
mFluor Violet 450 SE	FAB555MFV450
mFluor Violet 500 SE	FAB555MFV500
mFluor Violet 610 SE	FAB555MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB555-SP
Carrier Free	MAB555-500

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NB110-55234

TSLP Antibody - BSA Free

(1 Review)

(7 Publications)

0.05 mg

NB110-55234

Western Blot: TSLP Antibody [NB110-55234] - Total protein levels of TSLP in patients with AERD and subjects with CRS measured by using Western blotting of 3 representative samples per group. This image was submitted via customer Review.

Western Blot: TSLP Antibody [NB110-55234] - Western Blot Validation in Human Heart (Lane 1) and Human Prostate (Lane 2).Loading: 15 ug of lysates per lane. Antibodies: TSLP NB110-55234 (4 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

<b>Expression of Tagged Proteins Validation. </b>Western Blot: TSLP Antibody [NB110-55234] - Western Blot Validation of TSLP with Human Recombinant protein. Loading: 30ng of TSLP partial human recombinant protein per lane. Antibodies: TSLP NB110-55234 (Lane 1: 0.25 ug/mL, Lane 2: 0.5 ug/mL, Lane 3: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: TSLP Antibody - BSA Free [NB110-55234] - Validation in Mouse A-20 Cell Line. Loading: 15 ug of lysates per lane.Antibodies: TSLP (A: 0.5 ug/mL, B: 1 ug/mL, C: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry/ Immunofluorescence: TSLP Antibody - BSA Free [NB110-55234] - Validation of TSLP in Human Brain Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Brain Tissue labeling TSLP with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Immunohistochemistry: TSLP Antibody - BSA Free [NB110-55234] - Validation of TSLP in Human Brain Tissue. Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using anti-TSLP antibody at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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AF1398

TSLP Antibody [Unconjugated]

(2 Reviews)

(13 Publications)

100 μg

AF1398

1398-TS) stimulates proliferation in the BaF3 mouse pro-B cell line co-transfected with human IL-7 Ra and TSLP R in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human TSLP (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Human TSLP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1398). The ND₅₀ is typically 0.05-0.25 µg/mL." class="big_lightbox">

Recombinant Human TSLP (Catalog # <a class=

1398-TS) stimulates proliferation in the BaF3 mouse pro-B cell line co-transfected with human IL-7 Ra and TSLP R in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human TSLP (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Human TSLP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1398). The ND₅₀ is typically 0.05-0.25 µg/mL." alt="Recombinant Human TSLP (Catalog # 1398-TS) stimulates proliferation in the BaF3 mouse pro-B cell line co-transfected with human IL-7 Ra and TSLP R in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human TSLP (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Human TSLP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1398). The ND₅₀ is typically 0.05-0.25 µg/mL." class="big_thumb" />

1398-TS) was serially diluted and captured by Sheep Anti-Human TSLP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1398) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human TSLP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1398) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

1398-TS) was serially diluted and captured by Sheep Anti-Human TSLP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1398) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human TSLP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1398) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

1398-TS) was serially diluted and captured by Sheep Anti-Human TSLP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1398) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human TSLP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1398) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

1398-TS) was serially diluted and captured by Sheep Anti-Human TSLP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1398) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human TSLP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1398) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF1398U-100UG
Alexa Fluor 405	AF1398V-100UG
Alexa Fluor 488	AF1398G-100UG
Alexa Fluor 532	AF1398X-100UG
Alexa Fluor 594	AF1398T-100UG
Alexa Fluor 647	AF1398R-100UG
Alexa Fluor 700	AF1398N-100UG
Alexa Fluor 750	AF1398S-100UG
Alexa Fluor Plus 405	AF1398AFP405-100UG
Alexa Fluor Plus 488	AF1398AFP488-100UG
Alexa Fluor Plus 555	AF1398AFP555-100UG
Alexa Fluor Plus 594	AF1398AFP594-100UG
Alexa Fluor Plus 647	AF1398AFP647-100UG
Alexa Fluor Plus 680	AF1398AFP680-100UG
Allophycocyanin	AF1398A
Biotin	BAF1398
CoraFluor 1	AF1398CL1
DyLight 350	AF1398D
DyLight 405	AF1398E
DyLight 488	AF1398K
DyLight 550	AF1398L
DyLight 594	AF1398M
DyLight 650	AF1398W
DyLight 680	AF1398Y
DyLight 755	AF1398Z
FITC	AF1398F
HRP	AF1398H
Janelia Fluor 525	AF1398JF525
Janelia Fluor 549	AF1398I
Janelia Fluor 646	AF1398J
Janelia Fluor 669	AF1398JF669
PE	AF1398P
PerCP	AF1398C
mFluor Violet 450 SE	AF1398MFV450
mFluor Violet 500 SE	AF1398MFV500
mFluor Violet 610 SE	AF1398MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1398-SP

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NBP1-76754

TSLP Antibody - BSA Free

(1 Publication)

0.1 mg

NBP1-76754

Immunocytochemistry/ Immunofluorescence: TSLP Antibody - BSA Free [NBP1-76754] - Figure 2 Immunofluorescence Validation of TSLP in Mouse Brain TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed Mouse Brain Tissue labeling TSLP with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Reactivity:

Mu, Rt

Species Glossary

Reactivity: Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-76754AF350
Alexa Fluor 405	NBP1-76754AF405
Alexa Fluor 488	NBP1-76754AF488
Alexa Fluor 532	NBP1-76754AF532
Alexa Fluor 594	NBP1-76754AF594
Alexa Fluor 647	NBP1-76754AF647
Alexa Fluor 700	NBP1-76754AF700
Alexa Fluor 750	NBP1-76754AF750
Alexa Fluor Plus 405	NBP1-76754AFP405
Alexa Fluor Plus 488	NBP1-76754AFP488
Alexa Fluor Plus 555	NBP1-76754AFP555
Alexa Fluor Plus 594	NBP1-76754AFP594
Alexa Fluor Plus 647	NBP1-76754AFP647
Alexa Fluor Plus 680	NBP1-76754AFP680
Allophycocyanin	NBP1-76754APC
Biotin	NBP1-76754B
CoraFluor 1	NBP1-76754CL1
DyLight 350	NBP1-76754UV
DyLight 405	NBP1-76754V
DyLight 488	NBP1-76754G
DyLight 550	NBP1-76754R
DyLight 594	NBP1-76754DL594
DyLight 650	NBP1-76754C
DyLight 680	NBP1-76754FR
DyLight 755	NBP1-76754IR
FITC	NBP1-76754F
HRP	NBP1-76754H
Janelia Fluor 525	NBP1-76754JF525
Janelia Fluor 549	NBP1-76754JF549
Janelia Fluor 646	NBP1-76754JF646
Janelia Fluor 669	NBP1-76754JF669
PE	NBP1-76754PE
PerCP	NBP1-76754PCP
mFluor Violet 450 SE	NBP1-76754MFV450
mFluor Violet 500 SE	NBP1-76754MFV500
mFluor Violet 610 SE	NBP1-76754MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-76754-0.025mg

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NBP2-27326

TSLP Antibody (55N1D10) - BSA Free

(1 Publication)

0.1 mg

NBP2-27326

Reactivity:

Hu, Rb

Species Glossary

Reactivity: Hu, Rb

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-6861A

Alt Catalog# IMG-6861A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-27326AF350
Alexa Fluor 405	NBP2-27326AF405
Alexa Fluor 488	NBP2-27326AF488
Alexa Fluor 532	NBP2-27326AF532
Alexa Fluor 594	NBP2-27326AF594
Alexa Fluor 647	NBP2-27326AF647
Alexa Fluor 700	NBP2-27326AF700
Alexa Fluor 750	NBP2-27326AF750
Alexa Fluor Plus 405	NBP2-27326AFP405
Alexa Fluor Plus 488	NBP2-27326AFP488
Alexa Fluor Plus 555	NBP2-27326AFP555
Alexa Fluor Plus 594	NBP2-27326AFP594
Alexa Fluor Plus 647	NBP2-27326AFP647
Alexa Fluor Plus 680	NBP2-27326AFP680
Allophycocyanin	NBP2-27326APC
Biotin	NBP2-27326B
CoraFluor 1	NBP2-27326CL1
DyLight 350	NBP2-27326UV
DyLight 405	NBP2-27326V
DyLight 488	NBP2-27326G
DyLight 550	NBP2-27326R
DyLight 594	NBP2-27326DL594
DyLight 650	NBP2-27326C
DyLight 680	NBP2-27326FR
DyLight 755	NBP2-27326IR
FITC	NBP2-27326F
HRP	NBP2-27326H
Janelia Fluor 525	NBP2-27326JF525
Janelia Fluor 549	NBP2-27326JF549
Janelia Fluor 646	NBP2-27326JF646
Janelia Fluor 669	NBP2-27326JF669
PE	NBP2-27326PE
PerCP	NBP2-27326PCP
mFluor Violet 450 SE	NBP2-27326MFV450
mFluor Violet 500 SE	NBP2-27326MFV500
mFluor Violet 610 SE	NBP2-27326MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-27326SS
Azide and BSA Free	NBP2-80999

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NB110-55233

TSLP Antibody - BSA Free

(4 Publications)

0.1 mg

NB110-55233

Immunohistochemistry: TSLP Antibody - BSA Free [NB110-55233] - Figure 3 Immunohistochemistry Validation of TSLP in Human Brain Tissue Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using anti-TSLP antibody at 2.5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-55233AF350
Alexa Fluor 405	NB110-55233AF405
Alexa Fluor 488	NB110-55233AF488
Alexa Fluor 532	NB110-55233AF532
Alexa Fluor 594	NB110-55233AF594
Alexa Fluor 647	NB110-55233AF647
Alexa Fluor 700	NB110-55233AF700
Alexa Fluor 750	NB110-55233AF750
Alexa Fluor Plus 405	NB110-55233AFP405
Alexa Fluor Plus 488	NB110-55233AFP488
Alexa Fluor Plus 555	NB110-55233AFP555
Alexa Fluor Plus 594	NB110-55233AFP594
Alexa Fluor Plus 647	NB110-55233AFP647
Alexa Fluor Plus 680	NB110-55233AFP680
Allophycocyanin	NB110-55233APC
Biotin	NB110-55233B
CoraFluor 1	NB110-55233CL1
DyLight 350	NB110-55233UV
DyLight 405	NB110-55233V
DyLight 488	NB110-55233G
DyLight 550	NB110-55233R
DyLight 594	NB110-55233DL594
DyLight 650	NB110-55233C
DyLight 680	NB110-55233FR
DyLight 755	NB110-55233IR
FITC	NB110-55233F
HRP	NB110-55233H
Janelia Fluor 525	NB110-55233JF525
Janelia Fluor 549	NB110-55233JF549
Janelia Fluor 646	NB110-55233JF646
Janelia Fluor 669	NB110-55233JF669
PE	NB110-55233PE
PerCP	NB110-55233PCP
mFluor Violet 450 SE	NB110-55233MFV450
mFluor Violet 500 SE	NB110-55233MFV500
mFluor Violet 610 SE	NB110-55233MFV610

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DDX0804P-100

TSLP Antibody (210H1) - Azide and BSA Free

0.1 mg

DDX0804P-100

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	DDX0804B

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MAB1398

TSLP Antibody (258136)

(2 Publications)

100 μg

MAB1398

HAF007). A specific band was detected for TSLP at approximately 18 kDa (as indicated). This experiment was conducted under non-reducing conditions only and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human lung and kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TSLP Monoclonal Antibody (Catalog # MAB1398) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF007). A specific band was detected for TSLP at approximately 18 kDa (as indicated). This experiment was conducted under non-reducing conditions only and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human lung and kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TSLP Monoclonal Antibody (Catalog # MAB1398) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TSLP at approximately 18 kDa (as indicated). This experiment was conducted under non-reducing conditions only and using Immunoblot Buffer Group 1." class="big_thumb" />

Cytokine production induced by co-stimulation with TWEAK and TGF-beta 1 were steroid unresponsiveness. The mRNA levels of TSLP (A,C), CCL5 (D), and CCL17 (F) after 48 h of treatment and CCL2 (H) and IL-8 (J) after 2 h of treatment, analyzed by qRT–PCR. Data represent mean ± SD of two independent experiments. TSLP levels after 48 h of treatment assessed by immunoblotting (B, upper panel). The density of each band quantified by densitometry using ImageJ (version 6.1) (B, lower). The levels of CCL5 (E) and CCL17 (G) after 48 h of treatment, and CCL2 (I) and IL-8 (K) after 2 h of treatment in cell culture supernatants, analyzed by enzyme-linked immunosorbent assay (ELISA). Data represent the mean ± SD of three independent experiments. * p < 0.05, compared to untreated cultures as controls; † p < 0.05 compared with cultures in the absence of DEX. Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/1422-0067/25/21/11625), licensed under a CC-BY license. Not internally tested by R&D Systems.

MKP-1 is involved in steroid unresponsiveness induced by co-stimulation with TWEAK and TGF-beta 1 in BEAS-2B cells. The mRNA levels of MKP-1 (A), TSLP (C), and CCL5 (E), analyzed by qRT–PCR. Data represent mean ± SD of two independent experiments. MKP-1 and thymic stromal lymphopoietin (TSLP) expression after 48 h of treatment, assessed by immunoblotting (B,D, upper panel). Densitometric analysis of protein bands (B,D, lower). The level of CCL5 in cell culture supernatants after 48 h of treatment, analyzed using ELISA (F). Data represent mean ± SD of two independent experiments. * p < 0.05, compared to untreated cultures as controls; † p < 0.05 compared with cultures in the absence of DEX; ‡ p < 0.05 compared with control siRNA. Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/1422-0067/25/21/11625), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1398U-100UG
Alexa Fluor 405	FAB1398V-100UG
Alexa Fluor 488	FAB1398G-100UG
Alexa Fluor 532	FAB1398X-100UG
Alexa Fluor 594	FAB1398T-100UG
Alexa Fluor 647	FAB1398R-100UG
Alexa Fluor 700	FAB1398N-100UG
Alexa Fluor 750	FAB1398S-100UG
Alexa Fluor Plus 405	FAB1398AFP405-100UG
Alexa Fluor Plus 488	FAB1398AFP488-100UG
Alexa Fluor Plus 555	FAB1398AFP555-100UG
Alexa Fluor Plus 594	FAB1398AFP594-100UG
Alexa Fluor Plus 647	FAB1398AFP647-100UG
Alexa Fluor Plus 680	FAB1398AFP680-100UG
Allophycocyanin	FAB1398A
Biotin	FAB1398B
CoraFluor 1	FAB1398CL1
DyLight 350	FAB1398D
DyLight 405	FAB1398E
DyLight 488	FAB1398K
DyLight 550	FAB1398L
DyLight 594	FAB1398M
DyLight 650	FAB1398W
DyLight 680	FAB1398Y
DyLight 755	FAB1398Z
FITC	FAB1398F
HRP	FAB1398H
Janelia Fluor 525	FAB1398JF525
Janelia Fluor 549	FAB1398I
Janelia Fluor 646	FAB1398J
Janelia Fluor 669	FAB1398JF669
PE	FAB1398P
PerCP	FAB1398C
mFluor Violet 450 SE	FAB1398MFV450
mFluor Violet 500 SE	FAB1398MFV500
mFluor Violet 610 SE	FAB1398MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1398-SP

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NBP2-27325

TSLP Antibody (55N2D4) - BSA Free

0.1 mg

NBP2-27325

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-6862A

Alt Catalog# IMG-6862A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-27325AF350
Alexa Fluor 405	NBP2-27325AF405
Alexa Fluor 488	NBP2-27325AF488
Alexa Fluor 532	NBP2-27325AF532
Alexa Fluor 594	NBP2-27325AF594
Alexa Fluor 647	NBP2-27325AF647
Alexa Fluor 700	NBP2-27325AF700
Alexa Fluor 750	NBP2-27325AF750
Alexa Fluor Plus 405	NBP2-27325AFP405
Alexa Fluor Plus 488	NBP2-27325AFP488
Alexa Fluor Plus 555	NBP2-27325AFP555
Alexa Fluor Plus 594	NBP2-27325AFP594
Alexa Fluor Plus 647	NBP2-27325AFP647
Alexa Fluor Plus 680	NBP2-27325AFP680
Allophycocyanin	NBP2-27325APC
Biotin	NBP2-27325B
CoraFluor 1	NBP2-27325CL1
DyLight 350	NBP2-27325UV
DyLight 405	NBP2-27325V
DyLight 488	NBP2-27325G
DyLight 550	NBP2-27325R
DyLight 594	NBP2-27325DL594
DyLight 650	NBP2-27325C
DyLight 680	NBP2-27325FR
DyLight 755	NBP2-27325IR
FITC	NBP2-27325F
HRP	NBP2-27325H
Janelia Fluor 525	NBP2-27325JF525
Janelia Fluor 549	NBP2-27325JF549
Janelia Fluor 646	NBP2-27325JF646
Janelia Fluor 669	NBP2-27325JF669
PE	NBP2-27325PE
PerCP	NBP2-27325PCP
mFluor Violet 450 SE	NBP2-27325MFV450
mFluor Violet 500 SE	NBP2-27325MFV500
mFluor Violet 610 SE	NBP2-27325MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-27325SS
Azide and BSA Free	NBP2-80998

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MAB5551

TSLP Antibody (152640)

(1 Publication)

100 μg

MAB5551

555-TS) was serially diluted and captured by Rat Anti-Mouse TSLP Monoclonal Antibody (Catalog # MAB5551) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse TSLP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF555) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

555-TS) was serially diluted and captured by Rat Anti-Mouse TSLP Monoclonal Antibody (Catalog # MAB5551) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse TSLP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF555) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Mouse TSLP (Catalog # 555-TS) was serially diluted and captured by Rat Anti-Mouse TSLP Monoclonal Antibody (Catalog # MAB5551) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse TSLP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF555) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB5551U-100UG
Alexa Fluor 405	FAB5551V-100UG
Alexa Fluor 488	FAB5551G-100UG
Alexa Fluor 532	FAB5551X-100UG
Alexa Fluor 594	FAB5551T-100UG
Alexa Fluor 647	FAB5551R-100UG
Alexa Fluor 700	FAB5551N-100UG
Alexa Fluor 750	FAB5551S-100UG
Alexa Fluor Plus 405	FAB5551AFP405-100UG
Alexa Fluor Plus 488	FAB5551AFP488-100UG
Alexa Fluor Plus 555	FAB5551AFP555-100UG
Alexa Fluor Plus 594	FAB5551AFP594-100UG
Alexa Fluor Plus 647	FAB5551AFP647-100UG
Alexa Fluor Plus 680	FAB5551AFP680-100UG
Allophycocyanin	FAB5551A
Biotin	FAB5551B
CoraFluor 1	FAB5551CL1
DyLight 350	FAB5551D
DyLight 405	FAB5551E
DyLight 488	FAB5551K
DyLight 550	FAB5551L
DyLight 594	FAB5551M
DyLight 650	FAB5551W
DyLight 680	FAB5551Y
DyLight 755	FAB5551Z
FITC	FAB5551F
HRP	FAB5551H
Janelia Fluor 525	FAB5551JF525
Janelia Fluor 549	FAB5551I
Janelia Fluor 646	FAB5551J
Janelia Fluor 669	FAB5551JF669
PE	FAB5551P
PerCP	FAB5551C
mFluor Violet 450 SE	FAB5551MFV450
mFluor Violet 500 SE	FAB5551MFV500
mFluor Violet 610 SE	FAB5551MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB5551-SP
Carrier Free	MAB5551-500

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NBP3-28453

TSLP Antibody - (Research Grade tezepelumab Biosimilar) - Low Endotoxin, Azide and BSA Free

100 μg

NBP3-28453

Immobilized human TSLP His at 2 ug/mL can bind TSLP Antibody (tezepelumab), EC50=0.0518 ug/mL.

Tezepelumab reduced levels of IL13 in BALF of OVA asthma model on B-hTSLP/hTSLPR mice. The result showed significant IL13 inhibition effects at 15 mpk.

TSLP Antibody (tezepelumab)-induced FACS Blocking activity was evaluated using TSLPR/IL7R-CHO-K. The IC50 was approximately 0.0802 ug/mL.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

CHO Monoclonal

Host: CHO Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-28453AF350
Alexa Fluor 405	NBP3-28453AF405
Alexa Fluor 488	NBP3-28453AF488
Alexa Fluor 532	NBP3-28453AF532
Alexa Fluor 594	NBP3-28453AF594
Alexa Fluor 647	NBP3-28453AF647
Alexa Fluor 700	NBP3-28453AF700
Alexa Fluor 750	NBP3-28453AF750
Alexa Fluor Plus 405	NBP3-28453AFP405
Alexa Fluor Plus 488	NBP3-28453AFP488
Alexa Fluor Plus 555	NBP3-28453AFP555
Alexa Fluor Plus 594	NBP3-28453AFP594
Alexa Fluor Plus 647	NBP3-28453AFP647
Alexa Fluor Plus 680	NBP3-28453AFP680
Allophycocyanin	NBP3-28453APC
Biotin	NBP3-28453B
CoraFluor 1	NBP3-28453CL1
DyLight 350	NBP3-28453UV
DyLight 405	NBP3-28453V
DyLight 488	NBP3-28453G
DyLight 550	NBP3-28453R
DyLight 594	NBP3-28453DL594
DyLight 650	NBP3-28453C
DyLight 680	NBP3-28453FR
DyLight 755	NBP3-28453IR
FITC	NBP3-28453F
HRP	NBP3-28453H
Janelia Fluor 525	NBP3-28453JF525
Janelia Fluor 549	NBP3-28453JF549
Janelia Fluor 646	NBP3-28453JF646
Janelia Fluor 669	NBP3-28453JF669
PE	NBP3-28453PE
PerCP	NBP3-28453PCP
mFluor Violet 450 SE	NBP3-28453MFV450
mFluor Violet 500 SE	NBP3-28453MFV500
mFluor Violet 610 SE	NBP3-28453MFV610

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

TSLP Antibodies

TSLP Antibody [Unconjugated]

TSLP Antibody (152614)

TSLP Antibody - BSA Free

TSLP Antibody [Unconjugated]

TSLP Antibody - BSA Free

TSLP Antibody (55N1D10) - BSA Free

TSLP Antibody - BSA Free

TSLP Antibody (210H1) - Azide and BSA Free

TSLP Antibody (258136)

TSLP Antibody (55N2D4) - BSA Free

TSLP Antibody (152640)

TSLP Antibody - (Research Grade tezepelumab Biosimilar) - Low Endotoxin, Azide and BSA Free