![Western Blot: TSG101 Antibody [NBP2-98994] - Anti-TSG101 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: A431 Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lane D: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 49 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize2/TSG101-Antibody-Western-Blot-NBP2-98994-img0003.jpg "Western Blot: TSG101 Antibody [NBP2-98994] - Anti-TSG101 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: A431 Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lane D: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 49 kDa (We are unsure as to the identity of these extra bands).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human TSG101. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | TSG101 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um filtered solution in PBS |
| Preservative | No Preservative |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-98994AF350 | |
| Alexa Fluor 405 | NBP2-98994AF405 | |
| Alexa Fluor 488 | NBP2-98994AF488 | |
| Alexa Fluor 532 | NBP2-98994AF532 | |
| Alexa Fluor 594 | NBP2-98994AF594 | |
| Alexa Fluor 647 | NBP2-98994AF647 | |
| Alexa Fluor 700 | NBP2-98994AF700 | |
| Alexa Fluor 750 | NBP2-98994AF750 | |
| Biotin | NBP2-98994B | |
| DyLight 350 | NBP2-98994UV | |
| DyLight 405 | NBP2-98994V | |
| DyLight 488 | NBP2-98994G | |
| DyLight 550 | NBP2-98994R | |
| DyLight 594 | NBP2-98994DL594 | |
| DyLight 650 | NBP2-98994C | |
| DyLight 680 | NBP2-98994FR | |
| DyLight 755 | NBP2-98994IR | |
| FITC | NBP2-98994F | |
| HRP | NBP2-98994H | |
| Janelia Fluor 549 | NBP2-98994JF549 | |
| Janelia Fluor 646 | NBP2-98994JF646 |
Diseases for TSG101 Antibody (NBP2-98994)Discover more about diseases related to TSG101 Antibody (NBP2-98994).
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| Gene Symbol | TSG101 |
![Immunocytochemistry/Immunofluorescence: TSG101 Antibody [NBP2-98994] - Immunofluorescence staining of TSG101 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human TSG101 polyclonal antibody (1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize2/TSG101-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98994-img0002.jpg "Immunocytochemistry/Immunofluorescence: TSG101 Antibody [NBP2-98994] - Immunofluorescence staining of TSG101 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human TSG101 polyclonal antibody (1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.")
![Immunoprecipitation: TSG101 Antibody [NBP2-98994] - TSG101 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. Lane C: 0.5 mg HepG2 Whole Cell Lysate1 ul anti-TSG101 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-TSG101 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 44 kDa](http://images.novusbio.com/fullsize2/TSG101-Antibody-Immunoprecipitation-NBP2-98994-img0001.jpg "Immunoprecipitation: TSG101 Antibody [NBP2-98994] - TSG101 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. Lane C: 0.5 mg HepG2 Whole Cell Lysate1 ul anti-TSG101 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-TSG101 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 44 kDa")
![Western Blot: Alix Antibody [NBP1-90201] - Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PDCD6IP antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/images2/Alix-Antibody-Western-Blot-NBP1-90201-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Alix Antibody [NBP1-90201] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/images2/Alix-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90201-img0008.jpg)
![Immunohistochemistry-Paraffin: Alix Antibody [NBP1-90201] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/images2/Alix-Antibody-Immunohistochemistry-Paraffin-NBP1-90201-img0014.jpg)
![Western Blot: HGS Antibody (6D11) [H00009146-M01] - HGS monoclonal antibody (M01), clone 6D11 Analysis of HGS expression in K-562.](http://images.novusbio.com/images2/HGS-Antibody-(6D11)-Western-Blot-H00009146-M01-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: HGS Antibody (6D11) [H00009146-M01] - Analysis of monoclonal antibody to HGS on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/images2/HGS-Antibody-(6D11)-Immunocytochemistry-Immunofluorescence-H00009146-M01-img0006.jpg)
![Western Blot: HGS Antibody (6D11) [H00009146-M01] - Analysis of HGS over-expressed 293 cell line, cotransfected with HGS Validated Chimera RNAi ( Cat # H00009146-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with HGS monoclonal antibody (M01), clone 6D11 (Cat # H00009146-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/images2/HGS-Antibody-(6D11)-Western-Blot-H00009146-M01-img0008.jpg)
![Western Blot: VPS4A Antibody [NBP1-54618] - Human Fetal Lung, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/images2/VPS4A-Antibody-Western-Blot-NBP1-54618-img0007.jpg)
![Western Blot: VPS4A Antibody [NBP1-54618] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:312500 Positive Control: Human Muscle](http://images.novusbio.com/images2/VPS4A Antibody-Western Blot-NBP1-54618-img0006.jpg)
![Western Blot: SFRS5 Antibody [H00006430-B01P] - Analysis of SFRS5 expression in human liver.](http://images.novusbio.com/images2/SFRS5-Antibody-Western-Blot-H00006430-B01P-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: SFRS5 Antibody [H00006430-B01P] - Analysis of purified antibody to SFRS5 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/images2/SFRS5-Antibody-Immunocytochemistry-Immunofluorescence-H00006430-B01P-img0006.jpg)
![Immunohistochemistry: SFRS5 Antibody [H00006430-B01P] - Representative images from immune-histochemical staining of Ac-SRSF5, SRSF5, and Smurf1 in three serial sections of the same tumor and matched adjacent tissue. Scale bar, 50um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-018-04815-3), licensed under a CC-BY licence.](http://images.novusbio.com/images2/SFRS5-Antibody-Immunohistochemistry-H00006430-B01P-img0010.jpg)
![Western Blot: HARS Antibody [NBP1-89490] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/images2/HARS-Antibody-Western-Blot-NBP1-89490-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: HARS Antibody [NBP1-89490] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/images2/HARS-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89490-img0007.jpg)
![Immunohistochemistry-Paraffin: HARS Antibody [NBP1-89490] - Staining of human kidney shows positivity in cells in tubules.](http://images.novusbio.com/images2/HARS-Antibody-Immunohistochemistry-Paraffin-NBP1-89490-img0012.jpg)
![Immunohistochemistry-Paraffin: ATN1 Antibody [NBP1-90044] - Analysis in human cerebral cortex and liver tissues. Corresponding ATN1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/ATN1-Antibody-Immunohistochemistry-Paraffin-NBP1-90044-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: ATN1 Antibody [NBP1-90044] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/images2/ATN1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90044-img0012.jpg)
![Immunohistochemistry-Paraffin: ATN1 Antibody [NBP1-90044] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/images2/ATN1-Antibody-Immunohistochemistry-Paraffin-NBP1-90044-img0016.jpg)
![Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.](http://images.novusbio.com/images2/EGFR-Antibody-(DH8.3)---EGFRvIII-Flow-Cytometry-NBP2-50599-img0002.jpg)
![Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/images2/EGFR-Antibody-(DH8.3)---EGFRvIII-Flow-Cytometry-NBP2-50599-img0001.jpg)
![Western Blot: VPS28 Antibody (OTI1A8) - Azide and BSA Free [NBP2-74854] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VPS28.](http://images.novusbio.com/images2/VPS28-Antibody-(OTI1A8)---Azide-and-BSA-Free-Western-Blot-NBP2-74854-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: VPS28 Antibody (OTI1A8) - Azide and BSA Free [NBP2-74854] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY VPS28.](http://images.novusbio.com/images2/VPS28 Antibody (OTI1A8) - Azide and BSA Free-Immunocytochemistry-Immunofluorescence-NBP2-74854-img0004.jpg)
![Immunohistochemistry: VPS28 Antibody (OTI1A8) - Azide and BSA Free [NBP2-74854] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/images2/VPS28-Antibody-(OTI1A8)---Azide-and-BSA-Free-Immunohistochemistry-NBP2-74854-img0003.jpg)
![Western Blot: NEDD4 Antibody [NBP2-27201] - Analysis of NEDD4 in mouse embryo brain tissue lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-27201. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/images2/NEDD4-Antibody-Western-Blot-NBP2-27201-img0001.jpg)
![Western Blot: Exosome Component 9 Antibody [NBP1-71702] - Analysis of EXOSC9 in A. HepG2 cell lysate and B. MCF7 cell lysate.](http://images.novusbio.com/images2/Exosome-Component-9-Antibody-Western-Blot-NBP1-71702-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Exosome Component 9 Antibody [NBP1-71702] - EXOSC9 antibody was tested at 1:100 in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](http://images.novusbio.com/images2/Exosome-Component-9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-71702-img0007.jpg)
![Immunohistochemistry: Exosome Component 9 Antibody [NBP1-71702] - Staining of EXOSC9 in mouse prostate.](http://images.novusbio.com/images2/Exosome-Component-9-Antibody-Immunohistochemistry-NBP1-71702-img0008.jpg)
![Western Blot: DBT Antibody (OTI1G2) [NBP2-02042] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DBT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DBT.](http://images.novusbio.com/images2/DBT-Antibody-(OTI1G2)-Western-Blot-NBP2-02042-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: DBT Antibody (OTI1G2) [NBP2-02042] - Anti-DBT mouse monoclonal antibody (NBP2-02042) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DBT.](http://images.novusbio.com/images2/DBT-Antibody-(OTI1G2)-Immunocytochemistry-Immunofluorescence-NBP2-02042-img0003.jpg)
![Flow Cytometry: DBT Antibody (OTI1G2) [NBP2-02042] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DBT antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/images2/DBT-Antibody-(OTI1G2)-Flow-Cytometry-NBP2-02042-img0001.jpg)
![Western Blot: PDCD6 Antibody [NBP2-94061] - Analysis of 200ug extracts of HepG2 cells, using PDCD6 at a dilition of 1:1000.](http://images.novusbio.com/images2/PDCD6-Antibody-Western-Blot-NBP2-94061-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: PDCD6 Antibody [NBP2-94061] - Analysis of HeLa cells using PDCD6. Blue: DAPI for nuclear staining.](http://images.novusbio.com/images2/PDCD6-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94061-img0001.jpg)
![Immunohistochemistry-Paraffin: PDCD6 Antibody [NBP2-94061] - Paraffin-embedded rat kidney using PDCD6.](http://images.novusbio.com/images2/PDCD6-Antibody-Immunohistochemistry-Paraffin-NBP2-94061-img0002.jpg)
![Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Western-Blot-NB200-103-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Immunocytochemistry-Immunofluorescence-NB200-103-img0013.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Immunohistochemistry-Paraffin-NB200-103-img0005.jpg)
![Western Blot: CHMP4B Antibody [NBP2-87183] - Host: Rabbit. Target Name: CHMP4B. Sample Tissue: Human Lung Tumor. Antibody Dilution: 1ug/ml](http://images.novusbio.com/images2/CHMP4B-Antibody-Western-Blot-NBP2-87183-img0005.jpg)
![Immunohistochemistry-Paraffin: CHMP4B Antibody [NBP2-87183] - Rabbit Anti-CHMP4B antibody. Formalin Fixed Paraffin Embedded Tissue: Human Kidney. Primary antibody Concentration: 1:100. Secondary Antibody: Donkey anti-Rabbit-Cy3. Secondary Antibody Concentration: 1:200. Magnification: 20x.](http://images.novusbio.com/images2/CHMP4B-Antibody-Immunohistochemistry-Paraffin-NBP2-87183-img0001.jpg)
![Western Blot: CHMP4B Antibody [NBP2-87183] - Host: Rabbit. Target Name: CHMP4B. Sample Tissue: Human Hela. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/images2/CHMP4B-Antibody-Western-Blot-NBP2-87183-img0002.jpg)
![Western Blot: ARMER Antibody [NBP1-77198] - Analysis of ARMER in human bladder cell lysates with anti-ARMER (CT) using NBP1-77198 at (A) 0.5, (B) 1, and (C) 2 ug/ml.](http://images.novusbio.com/images2/ARMER-Antibody-Western-Blot-NBP1-77198-img0004.jpg)
![Immunohistochemistry-Paraffin: ARMER Antibody [NBP1-77198] - Staining of Human bladder tissue at 2 ug/ml.](http://images.novusbio.com/images2/ARMER-Antibody-Immunohistochemistry-Paraffin-NBP1-77198-img0002.jpg)
![Immunofluorescence: ARMER Antibody [NBP1-77198] - Immunofluorescence of ARMER in Mouse Intestine cells with ARMER antibody at 2 ug/mL.](http://images.novusbio.com/images2/ARMER-Antibody-Immunofluorescence-NBP1-77198-img0005.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")