TSG101 Antibody

Images

 
Genetic Strategies: Western Blot: TSG101 Antibody [NBP1-80659] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TSG101 antibody. Remaining relative ...read more
Immunocytochemistry/ Immunofluorescence: TSG101 Antibody [NBP1-80659] - Staining of human cell line A-431 shows localization to plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TSG101 Antibody [NBP1-80659] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.
Western Blot: TSG101 Antibody [NBP1-80659] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: TSG101 Antibody [NBP1-80659] - Staining of human prostate shows moderate cytoplasmic positivity in glandular and in smooth muscle cells.
Immunohistochemistry-Paraffin: TSG101 Antibody [NBP1-80659] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: TSG101 Antibody [NBP1-80659] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

TSG101 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: RMKEEMDRAQAELNALKRTEEDLKKGHQKLEEMVTRLDQEVAEVDKNIELLKKKDEELSSALEKMENQSENNDIDEVIIPTAPLYKQILNLYAEENAIEDTIFYLGEALRRGVIDLDVFLKHVRLLS
Marker
Exosome Marker
Specificity
Specificity of human TSG101 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TSG101
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.04-0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
Application Notes
WB reported in scientific literature (PMID: 25833224). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization, Use PFA/Triton X-100.
Control Peptide
TSG101 Recombinant Protein Antigen (NBP1-80659PEP)
Reviewed Applications
Read 2 Reviews rated 4
using
NBP1-80659 in the following applications:

Publications
Read Publications using NBP1-80659.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TSG101 Antibody

  • ESCRT-I complex subunit TSG101
  • TSG10
  • tumor susceptibility gene 10
  • tumor susceptibility gene 101 protein
  • tumor susceptibility gene 101
  • tumor susceptibility protein
  • VPS23

Background

Tumor susceptibility 101 protein (human TSG101 theoretical molecular weight 44kDa) is the mammalian homologue of the yeast protein Vps23 which plays a role in endosomal and multivesicular body trafficking. TSG101 forms part of the endosomal sorting complexes required for transport complex 1 (ESCRT-I), a cytosolic multiprotein complex consisting additionally of Vps28, Vps37 (A-D) and Mvb12 (A, B) or UBAP1 (1). TSG101 contains multiple domains including the amino terminal ubiquitin e2 variant (UEV) domain, a proline-rich (RRR) domain, a coiled coil (CC) domain, and a carboxy terminal alpha-helical/steadiness box (SB) domain (2). As part of the ESCRT-I complex, TSG101 interacts through its UEV domain with ubiquitinated membrane proteins and members of the ESCRT-0 complex. The UEV domain plays a critical role for various TSG101 functions such as protein sorting into multivesicular bodies and late endosomes, and in the process of viral budding. For example, TSG101 interacts with the intermediate-conductance, Ca2+ -activated K+ channel (KCa3.1), facilitating its targeting to the lysosome for degradation (3). Additionally, TSG101 has been implicated in the turnover of connexins such as connexins 43 and 45 (4). TSG101 plays a role in other cellular functions including transcriptional regulation, cytokinesis and cell growth (2).

Upon its initial discovery, TSG101 was recognized as a tumor suppressor protein due to the identification of deletions within the TSG101 gene in human breast carcinomas. However, re-examination of the initial findings argued against this function and supported that TSG101 promotes tumorigenesis (2). In agreement with this role, TSG101 expression is upregulated in several types of cancer including breast, ovarian, and colorectal carcinoma.

References

1. Schmidt, O., & Teis, D. (2012). The ESCRT machinery. Current Biology. https://doi.org/10.1016/j.cub.2012.01.028

2. Jiang, Y., Ou, Y., & Cheng, X. (2013). Role of TSG101 in cancer. Frontiers in Bioscience. https://doi.org/10.2741/4099

3. Balut, C. M., Gao, Y., Murray, S. A., Thibodeau, P. H., & Devor, D. C. (2010). ESCRT-dependent targeting of plasma membrane localized KCa3.1 to the lysosomes. American Journal of Physiology - Cell Physiology. https://doi.org/10.1152/ajpcell.00120.2010

4. Su, V., & Lau, A. F. (2014). Connexins: Mechanisms regulating protein levels and intercellular communication. FEBS Letters. https://doi.org/10.1016/j.febslet.2014.01.013

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC, IHC-P, KD

Publications for TSG101 Antibody (NBP1-80659)(2)

Reviews for TSG101 Antibody (NBP1-80659) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Rat.

Reviews using NBP1-80659:
Filter by Applications
WB
(2)
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Mouse
(1)
Rat
(1)
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Images Ratings Applications Species Date Details
Western Blot TSG101 NBP1-80659
Enlarge
4
reviewed by:
Anonymous
WB Rat 01/17/2018
View

Summary

ApplicationWestern Blot
Sample TestedRat astrocytes
SpeciesRat
LotB103539
Western Blot TSG101 NBP1-80659
Enlarge
4
reviewed by:
Anonymous
WB Mouse 12/08/2017
View

Summary

ApplicationWestern Blot
Sample TestedPrimary mouse hepatocytes
SpeciesMouse
LotB103539

Comments

Commentsmouse hepatocytes were treated with 100 ng/ml LPS for 4 hours

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TSG101 Antibody (NBP1-80659) (0)

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Anonymous
01/17/2018
Application: WB
Species: Rat

Anonymous
12/08/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol TSG101
Entrez
Uniprot