TSG-6 Antibody [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

TSG-6 Antibody [Alexa Fluor® 488] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TSG-6
  Trp18-Leu277
  Accession # P98066
• Specificity: Detects human TSG-6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 70% cross-reactivity with recombinant mouse TSG-6 is observed and less than 2% cross-reactivity with recombinant human TSG-14 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TSG-6 Antibody [Alexa Fluor® 488]

• Hyaluronate-binding protein
• TNF alpha-induced protein 6
• TNFAIP6
• TNF-stimulated gene 6 protein
• TSG6
• TSG-6
• TSG-6tumor necrosis factor alpha-inducible protein 6
• TSG6tumor necrosis factor-inducible gene 6 protein
• Tumor necrosis factor alpha-induced protein 6
• tumor necrosis factor, alpha-induced protein 6
• tumor necrosis factor-stimulated gene-6 protein

Background

TSG-6 (TNF-stimulated Gene 6), also known as TNFIP6 is a secreted, 35‑39 kDa group A member of the LINK-Module superfamily of proteins (1‑4). Human TSG-6 is synthesized as a 277 amino acid (aa) precursor. It contains a 17 aa signal sequence and a 260 aa mature region (5, 6). The mature region shows an N-terminal LINK module (amino acids 36‑129) and a C-terminal CUB (C1s/C1r; urchin embryonic growth factor; BMP1) domain (amino acids 135‑247). Link modules are alpha -helical, beta ‑sheet structures that bind hyaluronan (HA) and participate in extracellular matrix (ECM) assembly (7). Mature human TSG-6 shares 94% aa identity with both mouse and canine TSG-6. Cells reported to express TSG-6 include activated fibroblasts, synoviocytes, chondrocytes, neutrophils, proximal tubular epithelium, bronchial epithelium, endothelium, and visceral, plus vascular smooth muscle (2, 8). TSG-6 has multiple functions, many of which involve the ECM. It is suggested to stabilize HA-rich ECM. It does so by serving as an intermediary, or link, between the individual subunits of extracellular decameric pentraxin 3 and the surrounding hyaluronan matrix (9). It also provides structure and organization to hyaluronan. This is accomplished by a TSG-6 mediated transfer of an 80‑85 kDa HC subunit from I alpha I (inter‑ alpha ‑inhibitor) to HA. I alpha I is a four-component, 225 kDa serine protease inhibitor. It contains a protease inhibitor subunit (bikunin), two independent, accompaning protein chains (HC1 and HC2), and a short chondroitin sulfate linking moiety. TSG-6 is a cation-dependent catalyst for the removal, transfer, and subsequent covalent linkage of HC 1/2 to surrounding HA. This provides substance and reinforcement to the ECM (1, 2, 10‑12). The disassembly of I alpha I also leads to free bikunin, which in the “free” state becomes a potent inhibitor of serine proteases (8).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.