Tryptase gamma-1/TPSG1 Antibody (OTI1G1) - Azide and BSA Free

Western Blot: Tryptase gamma-1/TPSG1 Antibody (OTI1G1) - Azide and BSA Free [NBP2-74652] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TPSG1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TPSG1.

Immunohistochemistry-Paraffin: Tryptase gamma-1/TPSG1 Antibody (OTI1G1) - Azide and BSA Free [NBP2-74652] - Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TPSG1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, NBP2-01679).

Flow Cytometry: Tryptase gamma-1/TPSG1 Antibody (OTI1G1) - Azide and BSA Free [NBP2-74652] - Flow cytometric Analysis of Jurkat cells, using anti-TPSG1 antibody (NBP2-01679),(Red), compared to a nonspecific negative control antibody (Blue).

Immunohistochemistry-Paraffin: Tryptase gamma-1/TPSG1 Antibody (OTI1G1) - Azide and BSA Free [NBP2-74652] - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TPSG1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, NBP2-01679)

Immunohistochemistry-Paraffin: Tryptase gamma-1/TPSG1 Antibody (OTI1G1) - Azide and BSA Free [NBP2-74652] - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TPSG1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, NBP2-01679).

Flow Cytometry: Tryptase gamma-1/TPSG1 Antibody (OTI1G1) - Azide and BSA Free [NBP2-74652] - Flow cytometric Analysis of Hela cells, using anti-TPSG1 antibody (NBP2-01679),(Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: OTI1G1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Tryptase gamma-1/TPSG1 Antibody (OTI1G1) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Tryptase gamma-1/TPSG1 Antibody (OTI1G1) - Azide and BSA Free (NBP2-01679) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 20-283 of human TPSG1(NP_036599) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TPSG1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Tryptase gamma-1/TPSG1 Antibody (OTI1G1) - Azide and BSA Free

• EC 3.4.21
• EC 3.4.21.-
• gamma I
• gamma II
• lung tryptase
• mast cell protease II
• mast cell tryptase
• PRSS31skin tryptase
• Serine protease 31
• TMTpituitary tryptase
• TPSG1
• Transmembrane tryptase
• trpA
• tryptase gamma 1
• tryptase gamma I
• tryptase gamma II
• tryptase gamma
• Tryptase gamma1
• Tryptase gamma-1

Background

Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Tryptase is considered to be an important marker of mast cell activation as well as an important mediator of inflammation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TPSG1